Protein Alignment puf and T24B8.7

DIOPT Version :9

• Sequence 1: NP_001262923.1
• Gene: puf / 42935
• FlyBaseID: FBgn0039214
• Length: 3930
• Species: Drosophila melanogaster
• Sequence 2: NP_495931.4
• Gene: T24B8.7 / 174440
• WormBaseID: WBGene00011980
• Length: 2938
• Species: Caenorhabditis elegans
• Alignment Length: 2781
• Identity: 513/2781 - (18%)
• Similarity: 905/2781 - (32%)
• Gaps: 980/2781 - (35%)

  Fly   884 VCQPGNTLLWDLLQDDKIG--QLGESLALE-AEKALATLLCFSMDRQLRTKFIEGCL--YNV--- 940
            :|    .::|:|:...: |  :.|...|:| ..|....||....|:..|..|:...:  .||
 Worm   670 IC----NVIWELIHIAR-GPPRCGSFKAIEHGMKKHCDLLGMMFDKSERDSFLTNMMDGLNVDEG 729

  Fly   941 ANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAE--------RNHRMMQCFFN---NIRHYAR 994
            .|..:|:....:|.||..:|...|  .||.....:.        |..::.....:   ||||.|.
 Worm   730 FNEITVVYLFIMLDKLKRTFTPKR--STHGDETLSRVNELKASLRERKLADPLLDRLENIRHVAH 792

  Fly   995 RHAEVLITQNGEQQQQQL----------------------GGQLYSHKTQVSVR-LQFL----SS 1032
            ...|..:..|.:.|...:                      |...|....|.::| |::|    :|
 Worm   793 ERFEASLNANQDSQSNAVFESTNDVFGSHHVDNILTTSVYGCTDYGFVLQNTLRLLKWLHDVDAS 857

  Fly  1033 IFSTVGSPKSFRLTLEQLDA-----------LWE--WLAHDPECADCYFSWLQAQAKGGDQHALG 1084
            |.......:.|::.:||.|:           |:|  .:...||.:....:.|    .|.|...|.
 Worm   858 IVDVEYVQRLFKIFIEQSDSKPQEKTEIFGFLFEVRMVLMRPETSRAIITHL----CGMDIFTLQ 918

  Fly  1085 IEALQHL--YLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWK 1147
            |..|:..  |.::||.|..||...|.        ||  |...:.||..           ...:||
 Worm   919 ITGLRCFCKYWEELPILSNEESRPVE--------SF--IVKLQKDNEC-----------KLFVWK 962

  Fly  1148 IALRAQSNDV-----------SLAAIQ-----------YINMYYMGQQLRLEKEFVSQCMENLVQ 1190
            :.|..|.:||           .|..::           :::::....: :|:.|...:..:.|.:
 Worm   963 LILFNQFDDVVEKCMETFCERDLVLVESPYSVRHHTYSFLSVFSFYVE-KLKSELYKRSGKPLTE 1026

  Fly  1191 AA---TALESIDDE--------------------NALMRVQRGLLLLNTHLDTFRRRYAFHLRRW 1232
            .:   |.|...:||                    |.|:|.....:.|....:...|....|
 Worm  1027 ISYDTTELTMNEDEIKQHDVPLDGMLTETIVRALNRLVRFMTRFVELGNEKNHMIRENPSH---- 1087

  Fly  1233 AIEGKGIGSHS-----NLKNEGAGPPLRIVLQQAGLSE-KSLLQMHACD---LIADLKAEVSKWW 1288
               |..|..|:     .|||:...       ...||:. |:|:    ||   .:.:.|..::|
 Worm  1088 ---GSSISGHAVTFNIELKNDDDD-------IDCGLNAWKTLI----CDSSTTVGEFKVRIAK-- 1136

  Fly  1289 ESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFK-------------DNQIVYV 1340
                        .|...:|........:|     |:.::|:|..:             :...|::
 Worm  1137 ------------RLHFHEGMTEFTVHKRE-----DDLTMGEANLRYDFLTLQSVKLASETDSVFL 1184

  Fly  1341 S------LGGRGARRKESNLEHP--SMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQAS 1397
            |      :..:.:..:.|:|::.  |......::.||..::.:..:::.|..|... ||...:||
 Worm  1185 SEKLRILVKPKKSSNRRSDLQNHQYSQKKALREDFLPMSIISKCNFYDLLHELASN-GDKYIRAS 1248

  Fly  1398 TVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAG----GEEEQLATKRK 1458
            .             ||   :|.:|||.|.|             |:|:..|    .|:|.|..|..
 Worm  1249 V-------------RR---LLLLLPTQPSI-------------LKQITFGDMDDDEDEPLTIKIN 1284

  Fly  1459 QIKQKFRDLLDPNNLQKFMYSLHIVESLA----LTSSRRGESN------------GNVAMG--NT 1505
            .:..::...::|.   :.:|:|..:.|:.    ||:....::.            .|:..|  ..
 Worm  1285 NMCAEYMTPMEPG---RMLYALEAISSVVAPTRLTAQATEDATELTNALLEIDVIKNLTDGVLTK 1346

  Fly  1506 PEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDK 1570
            |..:.:.|.|.|.|.:              ..|::.::...:....:|.::.:
 Worm  1347 PNVIPLNKCSPGDRHS--------------IFERIIQIIRSVYTGRNTFIKMI------------ 1385

  Fly  1571 ENQPKQHSKRQKKGETFEQEKERPVGCSTP------PSPTPPPPALSVVERGDNKWSEAFVKCGG 1629
            .|:.:...:.:|..|...|.::.|.....|      .....|||.:      ||:.:.|:...|.
 Worm  1386 RNEFRIREEMRKTCERMLQSRQTPRSIDQPMDYALTKQQIMPPPCV------DNQNNPAYSSSGS 1444

  Fly  1630 LRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQ 1694
            :.....:||                        |.::|:                     ...:|
 Worm  1445 VVQFKSLFS------------------------SYIKII---------------------TSFMQ 1464

  Fly  1695 LMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFA----------M 1749
            ..::|..:..|.||.|                  :|:.|:.|.....:::..||          .
 Worm  1465 RWDLPDLIAFLESIRN------------------FQWIHVATESLLTSEIEPFAETNQNKGKKTR 1511

  Fly  1750 NILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLA 1814
            ......:.|..:..|  |.    |.||.:   |::.....:|.     :.|..:|   |:..:.|
 Worm  1512 ETASDLIRAVVKGER--WG----DECRLI---IVKKAFSLIRH-----MVREWVG---SHGDIAA 1559

  Fly  1815 PLLHKLIAL---------LPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLV------ 1864
            |||..|...         :.:..|..:..::.|..:.:.|||.:...|.....||...:
 Worm  1560 PLLETLFLNNSWVQFYQDVLVNSQQEAYRKYVQENIVKIGKDSFEITCVSLKMLLEMFMSLPLHD 1624

  Fly  1865 ----DTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDD-------------------- 1905
                |....:....:|      |:.|.|:::...|:|.....::|
 Worm  1625 GFTSDLNELQRRQRQH------CDGIVQTVVEIFYFESPRRCREDQKDIDWSQLGHKPIEIIRDQ 1683

  Fly  1906 --GLVGL----------------------LNLMSNLIKYDTTFKYTPKALSFIEQLIG-FLF-DM 1944
              .|:|.                      :.::|.|:.|.:.......:.:||:.:|. ||| ::
 Worm  1684 IKSLLGFKPRFCTTNNDLALTENIYAAAKMRMISCLLPYCSQSDIEDCSKAFIDAIINDFLFPEL 1748

  Fly  1945 PSPADRQKPKCKSASSRASAYDLLVELCRG-CATNYAYLHGRL-------LAQHKSGPKQPYPWD 2001
            |...|....: :|......|.:..::.... |..:|...|..|       :||      :.|...
 Worm  1749 PDIEDSMFEE-ESIRWEYQAREAAIQTLNAFCERSYKNSHQLLNMWSKFMIAQ------KTYDPS 1806

  Fly  2002 YWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMP---QARAAVLRVPP------NAARKHGPT 2057
            |.|...|| :...||:.|.|.||||.:.:|.|..:|   :...|:..:.|      |.|    ..
 Worm  1807 YRPIIRGR-QFDKVGMKNDGGTCYMNAMIQQLVHVPGLSRELIALQNIDPQLRWGDNTA----AL 1866

  Fly  2058 LLELQRMFAYLLESERKSYNPRSFCRVYQMD-HQPLNTGEQKDMAEFFIDLVSKLEDMTP--DLK 2119
            |.||||:||.|..::.::..|....:.::.: ..||||.:..|..:|:..|:.|.:::..  :|.
 Worm  1867 LCELQRVFAQLNFAQCQAIVPEGLWKEFRFEPDMPLNTKQHHDAIDFYSILLDKCDNVLKKLELP 1931

  Fly  2120 HLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCS 2184
            .|.:..|.|..|...:...|.|..::.::.:..........||:|:|:.......:||:|.|.|.
 Worm  1932 PLFQNRFFGKYSYEKICYGCWHRYKSPDEEFNCISLALSGDNLEEALENFLAAHVMEGENAYHCE 1996

  Fly  2185 QCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTL-MPQQ 2248
            :|.:|.....|..|.:||..:.....|:|:::|..:..|.|..|.||..::|..|:..:. :|.:
 Worm  1997 KCDEKKTTLNRTSFLELPSTMTIQLKRFTYDLVNNMIRKDNQLFRFPFEIDMTPYMTTSRHVPDE 2061

  Fly  2249 YKEE--RERRQKEKEGADGSGDGNDNEKAE-----------ATLDDDIEECYE------------ 2288
            :.::  .|......|..:.....:.|..||           .:|:...::.:.
 Worm  2062 HVQDLFDEMLYGNGEADEAPSPPHKNGVAEKPNLGSGSASTPSLESAQKKMFRRHRSSTMRLSQS 2126

  Fly  2289 -----------------YELVGVTVHTGTADGGHYYSFIKERT---KTSYHTHERWFLFNDAEVK 2333
                             ||||||..|:|.|..||||||||||.   :.|.| :.:|...||..|.
 Worm  2127 FANTSGFDTPSQQKPLIYELVGVLAHSGIATAGHYYSFIKERREEFRDSPH-YNKWHHINDMIVS 2190

  Fly  2334 PFDPSQIAAECFGGEMTSK-TYDSVTEKYLDFSFEKTNSAYMLFYERR-------LPEHLQRRHS 2390
            |...:.|....:||..|.: .:..:.|:...:      :||:||||::       :|.|:..|.:
 Worm  2191 PMSFNNIEDLWYGGTFTQEGVFIGLDERVRHW------NAYVLFYEKKRDEPTALIPRHIIDRLN 2249

  Fly  2391 ELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEI 2455
            :  |.|                       ::..||..|:..:.:||.|..|
 Worm  2250 D--VKP-----------------------KVTFDVSDEIMDDGEDKMKAVQ-------------- 2275

  Fly  2456 ADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASN 2520
              |..|:|..|.:|:
 Worm  2276 --DALKKDMSEARKL-------------------------------------------------- 2288

  Fly  2521 DQQPSTSKAAQKLQLFRPL---LNKELEDWIWQDNRQFLQDRNIFE----HTYFNFMWQICGHIP 2578
                       ::::|..:   :.|.|.|    |..:||.||:.|.    ..|.|.:      :|
 Worm  2289 -----------RIRMFNSMDLKMKKFLND----DYCKFLDDRDFFSFDLYQIYINSL------LP 2332

  Fly  2579 QSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYW 2643
            .....||....:.......||...|.:.......|.|:               .|..|..:.
 Worm  2333 LLKREETVEYTVTDLDKADFFKLAFEYIASYIIRVAWM---------------MFDEHRPKN--- 2379

  Fly  2644 PVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHA--HLYLEVETDEDDKELIGQASCVTRFIGSLI 2706
                            |.|....:|:.|...|.  .::.....:.::.|::      ||    ::
 Worm  2380 ----------------FPRAATDLIRLLLLRHPDNKMFFFKSLEANNSEML------TR----ML 2418

  Fly  2707 SLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHK-------QTDSID 2764
            ...||..||:..|        |..|         .||:.||::      |:|       ..||.|
 Worm  2419 ETTEHDIRASFWH--------CMRS---------ALRLWVLEN------GNKDENMMEPSPDSTD 2460

  Fly  2765 ISSD--------------NEDNSSEEALSVEKMRPASLDKMIALCASLV-ERSRGADFRLRLSPK 2814
            :..|              :||:.:||....:.|||:.:    ||.:.|| .:||....:..| |.
 Worm  2461 LDDDDEEDDEDDEDDDLEDEDSETEEMFRQDMMRPSPM----ALVSQLVMNQSRPPQLKPML-PV 2520

  Fly  2815 DFSAIAGGK---------GFPFLYQQIKDGINPHQTKHLIHAL----------------CRWDER 2854
            |.|.....:         ..||...:: :|...|.::.|:..|                |.
 Worm  2521 DLSKARSQRLNIVRRIVQVLPFRIHRM-EGSGRHYSRSLVEMLYMISRLNEFGKSVLHVCN---- 2580

  Fly  2855 LATQIIGMLFASVTKHTELC-------------------APFFKLLT------------------ 2882
             |.|::|. |......|..|                   ..:|:||.
 Worm  2581 -ALQLVGE-FLWEDYSTVFCRLRFSEDRIKGLGLWPYLPGLYFELLLDTMNRSLLHIIDPHLHYR 2643

  Fly  2883 -LLTETQGGPVGLPCFTQLILPRMWDAAEYCP----------QSVLDWLSLQATKNKIAHAWILQ 2936
             ||..|||.      |....|      |.||.          :||.|..||.   ::|...::.|
 Worm  2644 HLLMTTQGK------FMNESL------ALYCASREEHETTEGKSVNDEKSLH---SRIVLCYVRQ 2693

  Fly  2937 -----------SAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHS 2990
                       :|:::            :.||...||.|.:....:......|..|
 Worm  2694 IQLIVRSEVPDAAQEY------------IFNACQILLKAFLKDVQYMFTSIEHWSH--------- 2737

  Fly  2991 YRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMASSYMR 3055
                      |:...|.|..||:|     .|:.:.|...|.   ||:...:....:.:
 Worm  2738 ----------VIDFFIDLAQRLVR-----VDLSSLGACLLK---NLMWVGIAEDDDTV------- 2777

  Fly  3056 SLWELFHPRLSEPSV--------PAHHNKHALL--TFWHHSLVDCPENAAQVANCPEITRNI--- 3107
                       ||.|        ...:.|:..:  ..::..::..||..|.:..|.:..|:|
 Worm  2778 -----------EPGVLPVMLEWKETDYTKYRKMNEALFNIRIIKHPELRAVLLRCSKRFRSIFKM 2831

  Fly  3108 --AFNYILADH--DDAEIV--------------TYNRSMLPAYYGLLRLCCEQSRAL-TRQLSQH 3153
              |.|.|.:|.  |:::.|              ...||.:|:       .|..:|.: |::::..
 Worm  2832 SYAENDIDSDEPMDESDEVMIGPQLLKRATNSTDLTRSAVPS-------ACATARVIETKEINLE 2889

  Fly  3154 Q----NLQWAFKNITPHPT-QYAAAVD---ELFKLMALFATRHPDASEQEK 3196
            :    ||..|...:|..|: :...:||   |...::||  :.:|.|.:.||
 Worm  2890 EDLKANLSAASLEMTEQPSLEEVLSVDSSEEDISMIAL--SENPGAEDNEK 2938

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
puf	NP_001262923.1	peptidase_C19C	2013..2382	CDD:239124	108/434 (25%)
		UCH	2014..2377	CDD:278850	105/421 (25%)
		DUF3517	2723..>2877	CDD:288853	42/219 (19%)
T24B8.7	NP_495931.4	UBA	133..168	CDD:197551
		peptidase_C19C	1819..2234	CDD:239124	108/425 (25%)
		UCH	1819..2229	CDD:278850	105/420 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D625455at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm14202
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	2.010