Protein Alignment nAChRbeta2 and nAChRalpha2

DIOPT Version :9

• Sequence 1: NP_524483.1
• Gene: nAChRbeta2 / 42920
• FlyBaseID: FBgn0004118
• Length: 519
• Species: Drosophila melanogaster
• Sequence 2: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Alignment Length: 555
• Identity: 286/555 - (51%)
• Similarity: 374/555 - (67%)
• Gaps: 64/555 - (11%)

  Fly     1 MW-HWSLLCVFLLVPLANSTAPISFEANPDTKRLYDDLLSNYNRLIRPVVNNTETLTVWLGLKLS 64
            :| |...||:.|::.|...|.    :||||.||||||||||||||||||.|||:|:.|.|||:||
  Fly    19 IWRHCKPLCLLLVLLLLCETV----QANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLS 79

  Fly    65 QLIEVNLKNQVMTTNLWVKQRWFDYKLRWDPEEYGGVEQLYVPSEHIWVPDIVLYNNWDGNYEVT 129
            |||::|||:|::|||:|::..|.|:|.:|||.|||||.:|||||||||:||||||||.||.|.||
  Fly    80 QLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVT 144

  Fly   130 LMTKATLKYTGEVFWEPPAIYKSSCEMNVEYFPYDEQICFMKFGSWTYNGAQVDLKHLDQ-IPGS 193
            .||||.|.|||:|.|.||||:|||||::|.|||:|:|.||||||||||:|.|:||||:.| ....
  Fly   145 TMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKD 209

  Fly   194 NLVQVGIDLTEFYLSVEWDILEVPATKNEEYYPDTLEPFSDITFKLTMRRKTLFYTVNLIVPCVA 258
            |.|::||||.|:|.|||||||.|||.::|:|||...||:.||.|.:|:||||||||||||:|||.
  Fly   210 NKVEIGIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVG 274

  Fly   259 LTFLTVLVFYLPSDSGEKVTLCISILVSLTVFFLLLAEIIPPTSLAVPLLGKYLLFTMILVSLSV 323
            :::|:|||||||:|||||:.||||||:|.|:||||::||||.||||:|||||||||||:||.|||
  Fly   275 ISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSV 339

  Fly   324 WTTVCVLNIHFRSPSTHNMSPLVRKLFLHFMPKLMMMR---------------------RTQYTL 367
            ..|:.:||||:|.||||.|.|.:|..|:..:|||::||                     :|::..
  Fly   340 VITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVPKDLLRDLAANKINYGLKFSKTKFGQ 404

  Fly   368 PDYDD-------STPSN----------GYTNEIDVRDSISDFPSEFKDSQDGAYDNGMQ-----N 410
            ...|:       |:|.:          |..|.:.|..:.:.|     ....||...|:.     |
  Fly   405 ALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTATNRF-----SGLVGALGGGLSTLSGYN 464

  Fly   411 SVDS---------DNVIPRNLTP-EVLQALRAVRFIAQHIKDADKDNEIVEDWKFVSMVLDRFFL 465
            .:.|         .:|..|...| |:.:|:..|.||..|::..|:.|...:||.||:||:||.||
  Fly   465 GLPSVLSGLDDSLSDVAARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFL 529

  Fly   466 WLFTLSCVFGTLAIICQSPSLYDTRSPIDRQLSEI 500
            |||.::.:.||..|:.::|||||....||.|||::
  Fly   530 WLFMIASLVGTFVILGEAPSLYDDTKAIDVQLSDV 564

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRbeta2	NP_524483.1	LIC	9..479	CDD:273305	271/523 (52%)
		LGIC_ECD_nAChR_proto_alpha-like	29..247	CDD:349832	147/218 (67%)
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	147/218 (67%)
		LIC	47..544	CDD:273305	263/501 (52%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	250	1.000	Domainoid score	I2065
eggNOG	1	0.900	-	-		E1_KOG3645
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I1461
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm6399
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR18945
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
	9	8.970