DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment nAChRalpha2 and nAChRalpha4

DIOPT Version :9

Sequence 1:	NP_524482.1	Gene:	nAChRalpha2 / 42919	FlyBaseID:	FBgn0000039	Length:	576	Species:	Drosophila melanogaster
Sequence 2:	NP_001097669.2	Gene:	nAChRalpha4 / 40521	FlyBaseID:	FBgn0266347	Length:	539	Species:	Drosophila melanogaster

Alignment Length:	533	Identity:	276/533 - (51%)
Similarity:	365/533 - (68%)	Gaps:	41/533 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    39 VQANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQD 103
            |..||||||||||||||||:|:|||.|.||.:.|.:.|:||||||:|||:||:|||:|:|..|.|
  Fly     6 VAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYD 70

  Fly   104 HKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSS 168
            :|.:|:|.|||||..|:|||:|||.|||||||||||.:.||..|||.::..|.|.|.||||:|||
  Fly    71 YKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSS 135

  Fly   169 CEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGIDLREYYPSVEWDILGVP 233
            |||||.|||||:|||.:|||||||||.::||:|:.::.. .|.|.:|:||.|:|.|||||||.||
  Fly   136 CEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQG-SNVVAVGVDLSEFYMSVEWDILEVP 199

  Fly   234 AERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCIS 298
            |.|:||:|.||.|||.||.||||:||||||||||:||||:|||:|:||.||||:|||||:.|.||
  Fly   200 AVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSIS 264

  Fly   299 ILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIR 363
            ||:|..:||||:.||||.|||.:|||||||:|.|:||.:|:.:|:::||:|:|.|.||||.||::
  Fly   265 ILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVK 329

  Fly   364 SFFIKRLPKLLLMRVP-------KDLLRDLAANKINYGLKFSKTKFGQALMDEMQMNSG--GSSP 419
            ..||..||..|.::.|       |.||:|..|....|   :|.|.     :|.: ::||  .|.|
  Fly   330 RVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPY---YSATN-----IDRL-VSSGYNNSLP 385

  Fly   420 --DSLRRMQGRVGAGGCNGMH--VTTATNRFSGLVGALG----GGLSTLSGYNGLPSVLSGLDDS 476
              |..:.:......||...:|  |...|:..|..:.|:.    |.|       |:.|.:
  Fly   386 KEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPL-------GMKSPI------ 437

  Fly   477 LSDVA-ARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFLWLFMIASLVGT 540
            |::.| :..|...::.|:...|.||..|.:.|::.....:||.:||||:||||||:|.:|.:|||
  Fly   438 LNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGT 502

  Fly   541 FVILGEAPSLYDD 553
            ..|:.:||:||||
  Fly   503 AGIILQAPTLYDD 515

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	143/218 (66%)
nAChRalpha2	NP_524482.1	LIC	47..544	CDD:273305	263/514 (51%)
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	269/521 (52%)
		Neur_chan_LBD	12..227	CDD:280998	140/215 (65%)
		Neur_chan_memb	234..505	CDD:280999	117/292 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	250	1.000	Domainoid score	I2065
eggNOG	1	0.900	-	-		E1_KOG3645
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I1461
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm6399
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR18945
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
	9	8.970