Protein Alignment nAChRalpha2 and Chrne

DIOPT Version :10

• Sequence 1: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: XP_017452660.1
• Gene: Chrne / 29422
• RGDID: 2353
• Length: 547
• Species: Rattus norvegicus
• Alignment Length: 544
• Identity: 167/544 - (30%)
• Similarity: 260/544 - (47%)
• Gaps: 109/544 - (20%)

  Fly    40 QANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDH 104
            |...:...||..|..||:...|||....|||.:.|.:.|:.||.||.|::.|||:||:..||||:
  Rat    72 QGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEWQDY 136

  Fly   105 KFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSC 169
            :..:...::.||..|.|||||:|||:|||.||.||::.|......:::..|.|.|.||||::|:|
  Rat   137 RLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYRSTC 201

  Fly   170 EIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKV-EIGIDLREYYPSVEWDILGVP 233
            .::|.|||||.|.|.:.|.|.||:.::::.  |...:|..|.: :|.||...:..:.||.|
  Rat   202 AVEVTYFPFDWQNCSLIFRSQTYNAEEVEF--IFAVDDDGNAINKIDIDTAAFTENGEWAI---- 260

  Fly   234 AERHEKYYPCCAEPY----------PDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPAD 288
                 .|.|.....|          .|:.:.:.:|||.|||.:|:|:|||.||.|.:|.::|||.
  Rat   261 -----DYCPGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQ 320

  Fly   289 S-GEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRK 352
            : |:|..:.|::||:||:|..||::.||.|||::||||:||:|.|::..|.|:..:|:||:..|.
  Rat   321 AGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRT 385

  Fly   353 PSTHKMRPWIRSFFIKRLPKLLLMRVPKDLLRDLAANKINYGLKFSKTKFGQALMDEMQMNSGGS 417
            |:||...|.:|...::.||:||.:..|.:                               :.|.:
  Rat   386 PTTHATSPRLRQILLELLPRLLGLSPPPE-------------------------------DPGAA 419

  Fly   418 SPDS---------------LRRMQGRVGAGGCNGMHVT-TATNRFSGLVGALGGGLSTLSGYNGL 466
            ||..               |::.:..:...|....|.| ||
  Rat   420 SPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTA------------------------ 460

  Fly   467 PSVLSGLDDSLSDVAARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFLWL 531
                :.|..:||..|.      |:...:..|.|:....:.|:....|..||..:...:|.:..|.
  Rat   461 ----AALCQNLSAAAP------EVRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWA 515

  Fly   532 FMIASLVG-TFVILG----EAPSL 550
            .::...|| |.:.||    :.|.|
  Rat   516 ALVLFSVGSTLIFLGGYFNQVPDL 539

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	83/229 (36%)
		LIC	47..544	CDD:455710	162/525 (31%)
		Cys-loop	169..183	CDD:349832	8/13 (62%)
Chrne	XP_017452660.1	LGIC_ECD_nAChR_E	102..292	CDD:349831	71/200 (36%)
		Cys-loop	201..215	CDD:349831	8/13 (62%)
		Neur_chan_memb	300..528	CDD:460753	75/292 (26%)