Protein Alignment nAChRalpha2 and Chrna4

DIOPT Version :9

• Sequence 1: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: XP_038960311.1
• Gene: Chrna4 / 25590
• RGDID: 2346
• Length: 802
• Species: Rattus norvegicus
• Alignment Length: 634
• Identity: 249/634 - (39%)
• Similarity: 339/634 - (53%)
• Gaps: 118/634 - (18%)

  Fly     3 PGCCTTRPRPIALLAHIWRHCKPLCLLL--VLLLLCETVQANPDA-KRLYDDLLSNYNRLIRPVS 64
            ||.    |.|:.||        ||.|||  .||.....::....| :||...|.|.||:..|||:
  Rat   166 PGA----PPPLLLL--------PLLLLLGTGLLPASSHIETRAHAEERLLKRLFSGYNKWSRPVA 218

  Fly    65 NNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLP 129
            |.:|.|||:.||.::||||::.|:|::|||||::.||.|:|.:|||.:|..||.:.:|||.||.|
  Rat   219 NISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRP 283

  Fly   130 DIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDG 194
            ||||||||||::.||.:|||.|.|.|:|.||||||:||||.|||.:||||||.|.|||||||||.
  Rat   284 DIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDK 348

  Fly   195 DQIDLKHISQKNDKDNKVEIGIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRR 259
            .:|||..:..:          :|..:::.|.||.|:......:.:.|.||||.||||.:...:||
  Rat   349 AKIDLVSMHSR----------VDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRR 403

  Fly   260 KTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLL 324
            ..||||:||||||:.||.|:|||||||::.|||:.||||:|||.|:|.|||:||||||||.:||:
  Rat   404 LPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLI 468

  Fly   325 GKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVP---KDLLRDL 386
            |:||||||:.|.||:|||:.:||:|:|.|.||.|..|:|..|:..:|:||.|:.|   ||..|.|
  Rat   469 GEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRL 533

  Fly   387 ------AANK-----------------INYGLKFSKT--------------------KFGQALMD 408
                  .||.                 .|.||..:.|                    :.......
  Rat   534 IESMHKMANAPRFWPEPVGEPGILSDICNQGLSPAPTFCNPTDTAVETQPTCRSPPLEVPDLKTS 598

  Fly   409 EMQMNSGGSSPDSLRRMQGRVGAG------------------------GCNGMHVTTATNRFSGL 449
            |::..|...||.|....:...||.                        .|....:....::....
  Rat   599 EVEKASPCPSPGSCPPPKSSSGAPMLIKARSLSVQHVPSSQEAAEDGIRCRSRSIQYCVSQDGAA 663

  Fly   450 VGALGGGLSTLSGYNGLPSVLSGLDDS------------LSDVAARK----KYPFE-------LE 491
            ..|.....|:.:.....||.|...|.:            :|.|...|    |.|.:       |.
  Rat   664 SLADSKPTSSPTSLKARPSQLPVSDQASPCKCTCKEPSPVSPVTVLKAGGTKAPPQHLPLSPALT 728

  Fly   492 KAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFLWLFMIASLVGT 540
            :|:..|.:|..|::.:|...:..:||.:||||:||:|||:|:|..|:||
  Rat   729 RAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGT 777

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	111/219 (51%)
		LIC	47..544	CDD:273305	235/587 (40%)
Chrna4	XP_038960311.1	LGIC_ECD	199..405	CDD:355788	110/215 (51%)
		Cys-loop	323..337	CDD:349787	10/13 (77%)
		Neur_chan_memb	412..781	CDD:397193	120/366 (33%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1995
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	477	1.000	Inparanoid score	I1422
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm12327
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	8	7.970