Protein Alignment nAChRalpha2 and glra4b

DIOPT Version :10

• Sequence 1: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: XP_073805842.1
• Gene: glra4b / 192125
• ZFINID: ZDB-GENE-020402-2
• Length: 575
• Species: Danio rerio
• Alignment Length: 559
• Identity: 119/559 - (21%)
• Similarity: 201/559 - (35%)
• Gaps: 163/559 - (29%)

  Fly     4 GCCTT--------------RPRP---------------------IALLAHIWRHCKPLCLLLVLL 33
            |||..              ||.|                     ..:.:.|||      :||.||
Zfish    38 GCCAQSDGSGGETRVHQNGRPAPQKHICSLKFSHGTPEKQLLNSERMFSVIWR------ILLELL 96

  Fly    34 LLC-----------------ETVQANPDAKRLYDDLL----SNYNRLIR------PVSNNTDTVL 71
            |:|                 .:|:..|.:...:.|.|    |.|:..||      ||:...:..:
Zfish    97 LVCWMFEGVIRCVFSKELKSPSVRTKPMSPSDFLDKLMGKTSGYDARIRPNFKGPPVNVTCNIFI 161

  Fly    72 VKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPS--EHIWLPDIVLY 134
            ...|......:|..|       ||:|..:|.|.:..:  .||...:....||  :.||.||:...
Zfish   162 NSFGSITETTMDYRL-------NVFLRQQWNDPRLAY--KEYPDDSLDLDPSMLDSIWKPDLFFA 217

  Fly   135 NNADGEY-VVTTMTKAI-LHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQI 197
            |.....: .|||..|.: :...|.|:::.......||.:|::.||.|.|||.|:..|:.|..:.:
Zfish   218 NEKGANFHEVTTDNKLLRIFQNGNVLYSIRLTLILSCPMDLKNFPMDTQTCTMQLESFGYTMNDL 282

  Fly   198 DLKHISQKNDKDNKVEIGIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYP-------DIFFNI 255
            ..:.:.     :..|::..||          :|.....:.||....|.:.|.       ::.|:
Zfish   283 IFQWLD-----EGPVQVADDL----------MLPQFVLKEEKDLGYCTKHYNTGKFTCIEVKFH- 331

  Fly   256 TLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSG-EKIALCISILLSQTMFFLLISEIIPSTSL 319
             |.|:..:|.:.:.||.:....||.:.|::..|:. .::.|.|:.:|:.|.........:|..|.
Zfish   332 -LERQMGYYLIQMYIPSLLTVILSWVSFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSY 395

  Fly   320 ALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVPKDLLR 384
             :..:..::...:|.| .:.::....:|...|:         .:.||  ||.:.|     :...|
Zfish   396 -VKAIDIWMAVCLLFV-FAALLEYAAVNFVSRQ---------YKEFF--RLRRKL-----RQEQR 442

  Fly   385 DLAANKINYGLKFSKTKFGQALMDEMQMNSG---GSSPDSLRRMQGRVGAGGCNGMHVTTATNR- 445
            :.||:             |||...|.:..|.   |.:|.  |..|.:..|  |.......:.|:
Zfish   443 NRAAS-------------GQAGAAETKNKSNNVTGGTPS--RNAQRQCSA--CAREEQLASQNQD 490

  Fly   446 --FSGLVGALGGGLSTLSGYNGLPSVLSGLDDSLSDVAA 482
              |.|.               |:.:.||| |..||:.||
Zfish   491 LYFPGF---------------GIDTSLSG-DGPLSEAAA 513

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	54/239 (23%)
		LIC	47..544	CDD:455710	102/464 (22%)
		Cys-loop	169..183	CDD:349832	6/13 (46%)
glra4b	XP_073805842.1	None