Protein Alignment nAChRalpha2 and Chrne

DIOPT Version :10

• Sequence 1: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: XP_006532069.1
• Gene: Chrne / 11448
• MGIID: 87894
• Length: 494
• Species: Mus musculus
• Alignment Length: 558
• Identity: 167/558 - (29%)
• Similarity: 260/558 - (46%)
• Gaps: 117/558 - (20%)

  Fly    30 LVLLLLCETVQANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTN 94
            |:||.|....|...:...||..|..||:...|||....|||.:.|.:.|:.||.||.|::.|||:
Mouse     9 LLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTS 73

  Fly    95 VWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVW 159
            ||:..:|.|::..:...::.||..|.|||||:|||:|||.||.||::.|...:..:::..|.|.|
Mouse    74 VWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSW 138

  Fly   160 TPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKV-EIGIDLREYYP 223
            .||||::|:|.::|.|||||.|.|.:.|.|.||:.::::.  |...:|..|.: :|.||...:..
Mouse   139 LPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEF--IFAVDDDGNTINKIDIDTAAFTE 201

  Fly   224 SVEWDILGVPAERHEKYYPCCAEPY----------PDIFFNITLRRKTLFYTVNLIIPCVGISYL 278
            :.||.|         .|.|.....|          .|:.:.:.:|||.|||.:|:|:|||.||.|
Mouse   202 NGEWAI---------DYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGL 257

  Fly   279 SVLVFYLPADS-GEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVIT 342
            .:|.::|||.: |:|..:.|::||:||:|..||::.||.|||::||||:||:|.|::..|.|:..
Mouse   258 VLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNC 322

  Fly   343 IIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVPKD----------------LLR--DLAAN 389
            :|:||:..|.|:||...|.:|...::.||:||....|.:                |||  :|...
Mouse   323 VIVLNVSLRTPTTHATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILK 387

  Fly   390 KINYGLKF--SKTKFGQALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTATNRFSGLVGA 452
            |....|.|  .:.:.|......:..|.|.::|
Mouse   388 KPRSELVFEGQRHRHGTWTAAALCQNLGAAAP--------------------------------- 419

  Fly   453 LGGGLSTLSGYNGLPSVLSGLDDSLSDVAARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDW 517
                                                |:...:..|.|:....:.|:....|..||
Mouse   420 ------------------------------------EIRCCVDAVNFVAESTRDQEATGEELSDW 448

  Fly   518 GFVAMVMDRLFLWLFMIASLVG-TFVILG----EAPSL 550
            ..:...:|.:..|..::...|| |.:.||    :.|.|
Mouse   449 VRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDL 486

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	81/229 (35%)
		LIC	47..544	CDD:455710	158/529 (30%)
		Cys-loop	169..183	CDD:349832	8/13 (62%)
Chrne	XP_006532069.1	LGIC_ECD_nAChR_E	49..239	CDD:349831	69/200 (35%)
		Cys-loop	148..162	CDD:349831	8/13 (62%)
		Neur_chan_memb	247..475	CDD:460753	73/296 (25%)