Protein Alignment nAChRalpha2 and Chrna4

DIOPT Version :9

• Sequence 1: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: NP_056545.3
• Gene: Chrna4 / 11438
• MGIID: 87888
• Length: 629
• Species: Mus musculus
• Alignment Length: 617
• Identity: 247/617 - (40%)
• Similarity: 332/617 - (53%)
• Gaps: 111/617 - (17%)

  Fly    25 PLCLLLVLLLLCET--------VQANPDA-KRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQ 80
            |..|||.||||..|        ::....| :||...|.|.||:..|||:|.:|.|||:.||.::|
Mouse    10 PPLLLLPLLLLLGTGLLPASSHIETRAHAEERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQ 74

  Fly    81 LIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTT 145
            |||::.|:|::|||||::.||.|:|.:|||.:|..||.:.:|||.||.|||||||||||::.||.
Mouse    75 LIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTH 139

  Fly   146 MTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDN 210
            :|||.|.|.|:|.||||||:||||.|||.:||||||.|.|||||||||..:|||..:..:
Mouse   140 LTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSR----- 199

  Fly   211 KVEIGIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGI 275
                 :|..:::.|.||.|:......:.:.|.||||.||||.:...:||..||||:||||||:.|
Mouse   200 -----VDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLI 259

  Fly   276 SYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVV 340
            |.|:|||||||::.|||:.||||:|||.|:|.|||:||||||||.:||:|:||||||:.|.||:|
Mouse   260 SCLTVLVFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIV 324

  Fly   341 ITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVP---KDLLRDL------AANK------ 390
            ||:.:||:|:|.|.||.|..|:|..|:..:|:||.|:.|   ||..|.|      .||.
Mouse   325 ITVFVLNVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPE 389

  Fly   391 -----------INYGLKFSKT--------------------KFGQALMDEMQMNSGGSSPDSLRR 424
                       .|.||..:.|                    |.......|::..|...||.|...
Mouse   390 PESEPGILGDICNQGLSPAPTFCNRMDTAVETQPTCRSPSHKVPDLKTSEVEKASPCPSPGSCHP 454

  Fly   425 MQGR-----VGAGGCNGMHVTTATNRFSG-----------LVGALGGGLSTLSGYNGLPSVLS-- 471
            ....     :.|...:..||.::.....|           .|...|....|.|...|.|:.|.
Mouse   455 PNSSGAPVLIKARSLSVQHVPSSQEAAEGSIRCRSRSIQYCVSQDGAASLTESKPTGSPASLKTR 519

  Fly   472 ----GLDDSLSDVAARKKYPF------------------------ELEKAIHNVMFIQHHMQRQD 508
                .:.|..|......|.|.                        .|.:|:..|.:|..|::.:|
Mouse   520 PSQLPVSDQTSPCKCTCKEPSPVSPITVLKAGGTKAPPQHLPLSPALTRAVEGVQYIADHLKAED 584

  Fly   509 EFNAEDQDWGFVAMVMDRLFLWLFMIASLVGT 540
            ...:..:||.:||||:||:|||:|:|..|:||
Mouse   585 TDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGT 616

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	111/219 (51%)
		LIC	47..544	CDD:273305	237/586 (40%)
Chrna4	NP_056545.3	Neur_chan_LBD	39..245	CDD:280998	110/215 (51%)
		Neur_chan_memb	252..620	CDD:280999	122/365 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	420..459		6/38 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	503..529		6/25 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	476	1.000	Inparanoid score	I1470
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8871
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	8	7.860