Protein Alignment nAChRalpha2 and CHRNA4

DIOPT Version :9

• Sequence 1: NP_524482.1
• Gene: nAChRalpha2 / 42919
• FlyBaseID: FBgn0000039
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: NP_000735.1
• Gene: CHRNA4 / 1137
• HGNCID: 1958
• Length: 627
• Species: Homo sapiens
• Alignment Length: 611
• Identity: 242/611 - (39%)
• Similarity: 333/611 - (54%)
• Gaps: 105/611 - (17%)

  Fly    25 PLCLLL--VLLLLCETVQANPDA-KRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNL 86
            ||.|||  .||.....|:....| :||...|.|.||:..|||:|.:|.|||:.||.::||||::.
Human    14 PLLLLLGTGLLRASSHVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDE 78

  Fly    87 KDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAIL 151
            |:|::|||||::.||.|:|.:|||::|..||.:.:|||.||.|||||||||||::.||.:|||.|
Human    79 KNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHL 143

  Fly   152 HYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGI 216
            .:.|:|.||||||:||||.|||.:||||||.|.|||||||||..:|||.::..:          :
Human   144 FHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVNMHSR----------V 198

  Fly   217 DLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVL 281
            |..:::.|.||.|:......:.:.|.||||.||||.:...:||..||||:||||||:.||.|:||
Human   199 DQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVL 263

  Fly   282 VFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIIL 346
            |||||::.||||.||||:|||.|:|.|||:||||||||.:||:|:||||||:.|.||:|||:.:|
Human   264 VFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVL 328

  Fly   347 NIHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVP---KDLLRDL--AANKINYGLKFSKTKFGQ-- 404
            |:|:|.|.||.|..|:|..|:..:|:||||:.|   ||..|.|  :.:|:....:|.....|:
Human   329 NVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPP 393

  Fly   405 ----------------------------------ALMDEMQMNSGGSSPD--------------- 420
                                              .|..:..:.:..:||.
Human   394 ATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAP 458

  Fly   421 --------SLRRMQ--GRVGAGG--CNGMHVTTATNRFSGLVGALGGGLSTLSGYNGLPSVLSGL 473
                    |::.|.  |....||  |....:.....|......|.|.....|:..|...:.|...
Human   459 GLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPP 523

  Fly   474 DDSLSDVAARKKYPFE------------------------LEKAIHNVMFIQHHMQRQDEFNAED 514
            |.........||.|..                        |.:|:..|.:|..|::.:|...:..
Human   524 DQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVK 588

  Fly   515 QDWGFVAMVMDRLFLWLFMIASLVGT 540
            :||.:||||:||:|||:|:|..|:||
Human   589 EDWKYVAMVIDRIFLWMFIIVCLLGT 614

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	110/219 (50%)
		LIC	47..544	CDD:273305	233/586 (40%)
CHRNA4	NP_000735.1	Neur_chan_LBD	37..243	CDD:280998	109/215 (51%)
		Neur_chan_memb	250..618	CDD:280999	119/365 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	382..481		8/98 (8%)
		Ten_N	<423..>572	CDD:284015	21/148 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..561		9/64 (14%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	251	1.000	Domainoid score	I2107
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	485	1.000	Inparanoid score	I1448
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8639
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	8	7.870