DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nAChRalpha2 and Chrna3

DIOPT Version :9

Sequence 1:	NP_524482.1	Gene:	nAChRalpha2 / 42919	FlyBaseID:	FBgn0000039	Length:	576	Species:	Drosophila melanogaster
Sequence 2:	NP_660111.3	Gene:	Chrna3 / 110834	MGIID:	87887	Length:	499	Species:	Mus musculus

Alignment Length:	533	Identity:	230/533 - (43%)
Similarity:	329/533 - (61%)	Gaps:	45/533 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly    25 PLCLLLVLLLLCETVQANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQ 89
            ||.:|:::|:|.....|:....||:..|..:||.:||||:|.:..|:::..:.:|||:.::..:|
Mouse     9 PLSMLMLVLMLLPVASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQ 73

  Fly    90 ILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYT 154
            |:.||:||:..|.|:|.||.||:|.||..:.||:|.||.|||||||||||::.|...|||:|.||
Mouse    74 IMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYT 138

  Fly   155 GKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIG--ID 217
            |:|.|.|||||||||:|||.|||||.|.|.||||||:||..:|||            |.||  ::
Mouse   139 GEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL------------VLIGSSMN 191

  Fly   218 LREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLV 282
            |::|:.|.||.|:..|..:||..|.||.|.|.||.:::.:||..||||:||||||:.||:|:|||
Mouse   192 LKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLV 256

  Fly   283 FYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILN 347
            ||||:|.|||:.||||:|||.|:|.|:|:|.||||||.:||:|:||||||:.|.||:|||:.:||
Mouse   257 FYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLN 321

  Fly   348 IHYRKPSTHKMRPWIRSFFIKRLPKLLLMRVPKDLLRDLAANKINYGLKFSKTK-FGQALMDEMQ 411
            :|||.|:||.|..|:::.|:..||:::.|..|.....|....:..||.:.|... |.:|  |...
Mouse   322 VHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSNLNCFSRA--DSKS 384

  Fly   412 MNSGGSSPDSLRRMQGRVGAGGCNGMHVTTATNRFSGLVGALGGGLSTLSGYNGLPSVLSGLDDS 476
            ...|....|..        .|.|:  |.....:.||       ..|:..|....:.:||     |
Mouse   385 CKEGYPCQDGT--------CGYCH--HRRVKISNFS-------ANLTRSSSSESVDAVL-----S 427

  Fly   477 LSDVAARKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVMDRLFLWLFMIASLVGTF 541
            ||.::.      |:::||.:|.:|..:|:.|:.......||.:||||:||:|||:|::..::||.
Mouse   428 LSALSP------EIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTA 486

  Fly   542 VILGEAPSLYDDT 554
            .:..:.....|||
Mouse   487 GLFLQPLMARDDT 499

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha2	NP_524482.1	LGIC_ECD_nAChR_proto_alpha-like	44..263	CDD:349832	107/220 (49%)
nAChRalpha2	NP_524482.1	LIC	47..544	CDD:273305	221/499 (44%)
Chrna3	NP_660111.3	LIC	24..490	CDD:273305	222/507 (44%)
		LGIC_ECD_nAChR_A3	29..235	CDD:349817	107/217 (49%)
		Cys-loop	153..167	CDD:349817	10/13 (77%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	476	1.000	Inparanoid score	I1470
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm8871
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	11.980