DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nAChRalpha1 and chrna2a

DIOPT Version :9

Sequence 1:	NP_001262916.1	Gene:	nAChRalpha1 / 42918	FlyBaseID:	FBgn0000036	Length:	597	Species:	Drosophila melanogaster
Sequence 2:	NP_001035417.1	Gene:	chrna2a / 678575	ZFINID:	ZDB-GENE-040108-2	Length:	520	Species:	Danio rerio

Alignment Length:	550	Identity:	252/550 - (45%)
Similarity:	331/550 - (60%)	Gaps:	72/550 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 LFAAVFIALH----FATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVN 65
            |:..:.||.|    .:.|.:......:|:..|...||:..|||.|.||.:.||.||.::|||||:
Zfish    13 LWMLILIADHVQPVLSDGWIHAHAEDKLFHKLFIGYNKWSRPVRNISDVVIVKFGLSIAQLIDVD 77

  Fly    66 LKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAI 130
            .|||:||||||::||||||||:|||.::..|.::.||||.||:|||||||||||.:.||.||||.
Zfish    78 EKNQMMTTNVWLKQEWNDYKLRWNPSEFDNVTSIRVPSEMIWVPDIVLYNNADGEFAVTHMTKAH 142

  Fly   131 LHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGI 195
            |.|||||.|.|||||||.|.|||.:||||:|.|.|||||||||...:||         :.||..:
Zfish   143 LFHTGKVSWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL---------EQIEGTV 198

  Fly   196 DLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVL 260
            ||:||:.|.||.|:......|.|.|.||.|.|.||.:...:||..||||:||||||:.||.|:||
Zfish   199 DLKDYWESGEWAIINAVGTYNTKKYDCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVL 263

  Fly   261 VFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVL 325
            |||||||.||||:||||:|||||||.||:.||||.|||.:||:|:||||||:.||||:|:|:.||
Zfish   264 VFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVL 328

  Fly   326 NVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKE-EPEEDQPPEVLTDVYHLPPDVDKFVNYD 389
            ||:.|||.||.|..||..:|:..:|:.|.:.||:.| :|::..........:.....||..|..|
Zfish   329 NVHHRSPSTHSMPRWVHSVFLDHIPRWLFMRRPEPEKKPKKTANLSTSKSWFQQETGVDGLVCQD 393

  Fly   390 SKRFSGDYGI--------PALPASH---------RFDLAAAGGISAHCFAEPPLPSSLPLPGADD 437
            .     ::||        |:..:.|         :::|...|.:...|.....|.:.:..||.
Zfish   394 M-----EFGIGLSTSISSPSATSLHLSDSEPLLQKYELQHLGHVVNLCQHPAKLGNLVSEPGL-- 451

  Fly   438 DLFSPSGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEE 502
             ||||..|                                 :.:||.|:||.|::.:|:..||:|
Zfish   452 -LFSPCVL---------------------------------RALEGVRYIADHLRAEDEDFSVKE 482

  Fly   503 DWKYVAMVLDRMFLWIFAIACVVGTALIIL 532
            ||||||||:||:|||:|.|.|::||..:.|
Zfish   483 DWKYVAMVIDRIFLWMFIIVCLLGTIGLFL 512

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	Neur_chan_LBD	25..240	CDD:280998	119/214 (56%)
		LIC	27..530	CDD:273305	246/520 (47%)
		Neur_chan_memb	247..530	CDD:280999	122/300 (41%)
chrna2a	NP_001035417.1	LIC	9..513	CDD:273305	251/549 (46%)
		Neur_chan_LBD	37..243	CDD:280998	119/214 (56%)
		Neur_chan_memb	250..511	CDD:280999	122/301 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	250	1.000	Domainoid score	I2065
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I1461
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm6399
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2381
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.810