Protein Alignment nAChRalpha1 and chrna4b

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_001041528.1
• Gene: chrna4b / 556619
• ZFINID: ZDB-GENE-090505-3
• Length: 627
• Species: Danio rerio
• Alignment Length: 624
• Identity: 245/624 - (39%)
• Similarity: 334/624 - (53%)
• Gaps: 153/624 - (24%)

  Fly    26 KRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNP 90
            :||...|..|||:|.|||.|.||.:.|..||.::|||||:.|||:|||||||:||||||||:|||
Zfish    32 ERLLQSLFINYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWNDYKLRWNP 96

  Fly    91 DDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEY 155
            ::|..|.::.:|||.||.|||||||||||::.||.:|||.:.:.|::.|||||||||.|.|||.:
Zfish    97 EEYENVTSIRIPSEIIWRPDIVLYNNADGDFAVTHLTKAQVFYNGRIKWKPPAIYKSSCSIDVTF 161

  Fly   156 FPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWDIMRVPAVRNEKFY 220
            ||||:|.|.|||||||||...:||  :...:|       :|..||:.|.||.|:......|.|.|
Zfish   162 FPFDQQNCKMKFGSWTYDRAKIDL--ISMASD-------VDQMDYWESGEWVIINAVGTYNIKKY 217

  Fly   221 SCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVF 285
            .||.|.|.||.::..:||..||||:||||||:.||.|:||||||||:..|||:||||:|||||||
Zfish   218 ECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVF 282

  Fly   286 FLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILP 350
            .||:.||||.|||.:||:|:||||||:.||||:::|:.||||:.||..||.|..||::||:.::|
Zfish   283 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHSMPHWVRQLFLHLVP 347

  Fly   351 KLLCIERP------------------------------------------------KKEEPEEDQ 367
            :.|.::||                                                ::::.::|.
Zfish   348 RYLFMKRPPASGKRNCGKLIEMMHKQANSQSMFLRNNILDISPQSPIPHLGPTSAAQQDQVQQDS 412

  Fly   368 --------------------------------------------------PPEVLTDVYH-LPPD 381
                                                              |..:|..:.| :|.|
Zfish   413 TSPKPLFFCSSPSSQYSILQEENSQTPQTPVLSYTCAYTNSIFASSTTSFPDTLLGTLLHTIPQD 477

  Fly   382 VDKFVNYDSKRFSGDYGIPALPAS------------------HRFDLAAAGGISAHCFAEPPLPS 428
            ..|....|.:..|.:........|                  .:.||..||..:.|     .:||
Zfish   478 GAKSTECDFQGHSAESVFMEAKESVLGAGSCQHCLTSEGTSLQKLDLDEAGRCTRH-----NMPS 537

  Fly   429 SLPLPGADDDLFSPSGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKN 493
            :        .:||||      |.|.....||...::||        :.:.|.:||.::||.|::.
Zfish   538 A--------QIFSPS------SDGSTDLNAAEPVSNLS--------QSLLKALEGVQYIADHLRA 580

  Fly   494 KDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALIIL 532
            :|...||.|||||||||:||:|||:|.:.|::||..:.|
Zfish   581 EDSDFSVREDWKYVAMVIDRIFLWMFVLVCILGTVGLFL 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	115/215 (53%)
		LIC	27..530	CDD:455710	244/619 (39%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
chrna4b	NP_001041528.1	LGIC_ECD	31..237	CDD:475126	115/213 (54%)
		Neur_chan_memb	244..618	CDD:460753	124/400 (31%)