Protein Alignment nAChRalpha1 and nAChRbeta1

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_523927.2
• Gene: nAChRbeta1 / 38545
• FlyBaseID: FBgn0000038
• Length: 521
• Species: Drosophila melanogaster
• Alignment Length: 558
• Identity: 239/558 - (42%)
• Similarity: 330/558 - (59%)
• Gaps: 53/558 - (9%)

  Fly     3 SVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLK 67
            |.|..::.:.:.|:... |:.|.:||..||...||:|||||.|.:.::.|:.||...|||:||.|
  Fly     7 SWLLCSILVLVAFSLVS-ASEDEERLVRDLFRGYNKLIRPVQNMTQKVGVRFGLAFVQLINVNEK 70

  Fly    68 NQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILH 132
            ||||.:|||:...|.||:|:|:..||||:..|.:|.:.:|.|||||:|||||||||...:..:::
  Fly    71 NQIMKSNVWLRLVWYDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLIY 135

  Fly   133 HTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDL 197
            .||:|:|.|||||:|.|.|||.|||||:|||.|||||||::|..|.|    ...::.|.   :||
  Fly   136 PTGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSL----ALYNNKNF---VDL 193

  Fly   198 QDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVF 262
            .||:.|..|||:.|||..|............||.|.:.:||||||||||||:|.|.||||.||||
  Fly   194 SDYWKSGTWDIIEVPAYLNVYEGDSNHPTETDITFYIIIRRKTLFYTVNLILPTVLISFLCVLVF 258

  Fly   263 YLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNV 327
            |||:::|||::|.|||||||.||.||:::|:|||||.:||:.||||||.::.|:|::||:.::|.
  Fly   259 YLPAEAGEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIINW 323

  Fly   328 NFRSPVTHRMAPWVQRLFIQILPKLLCIERPKK-------EEPEEDQPPEVLTDVYHLPPDVDKF 385
            |||.|.||||..:::.:|:..||..|.::||:|       |.|....|.       |..|
  Fly   324 NFRGPRTHRMPMYIRSIFLHYLPAFLFMKRPRKTRLRWMMEMPGMSMPA-------HPHP----- 376

  Fly   386 VNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDIS 450
                      .||.||....|   ::|.||..:.  .|....|.|..|....:....||....:.
  Fly   377 ----------SYGSPAELPKH---ISAIGGKQSK--MEVMELSDLHHPNCKINRKVNSGGELGLG 426

  Fly   451 PGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMF 515
            .||...:.::.:..|||        |..|..|...|||:|::|:|.:....|||||||||:||:.
  Fly   427 DGCRRESESSDSILLSP--------EASKATEAVEFIAEHLRNEDLYIQTREDWKYVAMVIDRLQ 483

  Fly   516 LWIFAIACVVGTALIILQAPSLY---DQSQPIDILYSK 550
            |:||.|....||..|::.||.::   ||.:.|:|...|
  Fly   484 LYIFFIVTTAGTVGILMDAPHIFEYVDQDRIIEIYRGK 521

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	107/217 (49%)
		LIC	27..530	CDD:455710	226/509 (44%)
		Cys-loop	149..163	CDD:349832	10/13 (77%)
nAChRbeta1	NP_523927.2	LIC	8..500	CDD:455710	231/534 (43%)
		LGIC_ECD_nAChR_proto_beta-like	27..236	CDD:349833	105/215 (49%)
		Cys-loop	152..166	CDD:349833	10/13 (77%)