Protein Alignment nAChRalpha1 and HTR3A

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_998786.3
• Gene: HTR3A / 3359
• HGNCID: 5297
• Length: 510
• Species: Homo sapiens
• Alignment Length: 550
• Identity: 136/550 - (24%)
• Similarity: 239/550 - (43%)
• Gaps: 119/550 - (21%)

  Fly    23 PDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLK 87
            |...||.|.||:||.:.:|||.:.....||.:.:.:..:::|:.|||::||.:|..|.|.|..|:
Human    32 PALLRLSDYLLTNYRKGVRPVRDWRKPTTVSIDVIVYAILNVDEKNQVLTTYIWYRQYWTDEFLQ 96

  Fly    88 WNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEID 152
            |||:|:..:..|.:|::.||:|||::....|......| ....:.|.|:|....|....:.|.:|
Human    97 WNPEDFDNITKLSIPTDSIWVPDILINEFVDVGKSPNI-PYVYIRHQGEVQNYKPLQVVTACSLD 160

  Fly   153 VEYFPFDEQTCFMKFGSW--TYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISV-----EWDIMR 210
            :..||||.|.|.:.|.||  |.....:.|..|.:...||.            ||     ||:::.
Human   161 IYNFPFDVQNCSLTFTSWLHTIQDINISLWRLPEKVKSDR------------SVFMNQGEWELLG 213

  Fly   211 V-PAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISL 274
            | |..|  :|.......|.::.|.:.:||:.|||.|:|::|.:.:..:.::.||||.:|||::|.
Human   214 VLPYFR--EFSMESSNYYAEMKFYVVIRRRPLFYVVSLLLPSIFLMVMDIVGFYLPPNSGERVSF 276

  Fly   275 CISILLSLTVFFLLLAEIIPPTSLTVPLLGK--------------------------------YL 307
            .|::||..:||.:::::.:|.|::..||:||                                |.
Human   277 KITLLLGYSVFLIIVSDTLPATAIGTPLIGKAPPGSRAQSGEKPAPSHLLHVSLASALGCTGVYF 341

  Fly   308 LFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVL 372
            :..|.|:.:|:..||.::.:..:..:...:..|::.|.::.:..|||:    :|:....:||
Human   342 VVCMALLVISLAETIFIVRLVHKQDLQQPVPAWLRHLVLERIAWLLCL----REQSTSQRPP--- 399

  Fly   373 TDVYHLPPDVDKFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADD 437
                                       ....|:...|.:|.|...:|            :.|..|
Human   400 ---------------------------ATSQATKTDDCSAMGNHCSH------------MGGPQD 425

  Fly   438 DLFSPSGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEE 502
            ...||   ....||...|..|:.|...|        .:|:..       |.|.::.:|:...|..
Human   426 FEKSP---RDRCSPPPPPREASLAVCGL--------LQELSS-------IRQFLEKRDEIREVAR 472

  Fly   503 DWKYVAMVLDRMFLWIFAIACVVGTALIIL 532
            ||..|..|||::...|:.:|.:..:..:::
Human   473 DWLRVGSVLDKLLFHIYLLAVLAYSITLVM 502

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	68/225 (30%)
		LIC	27..530	CDD:455710	135/542 (25%)
		Cys-loop	149..163	CDD:349832	7/13 (54%)
HTR3A	NP_998786.3	LIC	23..504	CDD:273305	136/549 (25%)