Protein Alignment nAChRalpha1 and Chrnb4

DIOPT Version :9

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_434693.1
• Gene: Chrnb4 / 25103
• RGDID: 2351
• Length: 495
• Species: Rattus norvegicus
• Alignment Length: 559
• Identity: 234/559 - (41%)
• Similarity: 323/559 - (57%)
• Gaps: 92/559 - (16%)

  Fly     4 VLFAAVFIALHFATGGLANPDAKRLYDDLL--SNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNL 66
            :|..::|..|......|||.:.| |.||||  :.||.||||..::|..:::::.|.|||||.||.
  Rat     6 LLLVSLFSLLQDGDCRLANAEEK-LMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNE 69

  Fly    67 KNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAIL 131
            :.|||||::|::|||.||:|.||...|.||:.|.:|::.:|||||||||||||.|||::.|..|:
  Rat    70 REQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIV 134

  Fly   132 HHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGID 196
            ...|.:.|.|||||||.|:|:|::||||:|.|.:||.|||||...:|:.....||..|
  Rat   135 RSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMD------- 192

  Fly   197 LQDYYISVEWDIMRVPAVRNEKFYSCCEEP-YLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVL 260
              |:..|.||||:.:|..|...    .::| |:|:.::..::||.||||:|||||||.|:.|::|
  Rat   193 --DFTPSGEWDIVALPGRRTVN----PQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAIL 251

  Fly   261 VFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVL 325
            |||||||.|||::||||:||:||.|.||:::|:|||||.:||:||||||||:|||.|:|.|:.||
  Rat   252 VFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVL 316

  Fly   326 NVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDS 390
            ||:.|||.||.||.||:..|:..||..|.::||..|
  Rat   317 NVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLE----------------------------- 352

  Fly   391 KRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDL------FSPSGLNGDI 449
                                     :|   ....|.||.|.|..||...      .|||.|.|..
  Rat   353 -------------------------VS---LVRVPHPSQLHLATADTAATSALGPTSPSNLYGSS 389

  Fly   450 SPGCCPAAAAAAAADLSPTFEKP----------YAREMEKTIEGSRFIAQHVKNKDKFESVEEDW 504
            .....|..||..:|..|.|...|          :..::::.:||..|||||:::.|:.:||.|||
  Rat   390 MYFVNPVPAAPKSAVSSHTAGLPRDARLRSSGRFREDLQEALEGVSFIAQHLESDDRDQSVIEDW 454

  Fly   505 KYVAMVLDRMFLWIFAIACVVGTALIILQAPSLYDQSQP 543
            |:||||:||:|||:|...|::||..:.|  |.|:....|
  Rat   455 KFVAMVVDRLFLWVFVFVCILGTMGLFL--PPLFQIHAP 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	Neur_chan_LBD	25..240	CDD:280998	99/217 (46%)
		LIC	27..530	CDD:273305	223/521 (43%)
		Neur_chan_memb	247..530	CDD:280999	119/298 (40%)
Chrnb4	NP_434693.1	LIC	21..483	CDD:273305	228/534 (43%)
		LGIC_ECD	53..230	CDD:355788	85/189 (45%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	229	1.000	Domainoid score	I2385
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.920