Protein Alignment nAChRalpha1 and Chrna5

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_058774.2
• Gene: Chrna5 / 25102
• RGDID: 2347
• Length: 467
• Species: Rattus norvegicus
• Alignment Length: 527
• Identity: 209/527 - (39%)
• Similarity: 291/527 - (55%)
• Gaps: 116/527 - (22%)

  Fly    18 GGL-----ANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWV 77
            |||     |......|:.||..:|.|.:|||.:.||::.:|.||.:|||:||:.|||:||||||:
  Rat    35 GGLPELSSAAKHEDSLFRDLFEDYERWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTNVWL 99

  Fly    78 EQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPP 142
            :|||.|.||:||||||||:..:.|||:.:|:|||||::||||.:| ...||.::.:.|.|.|..|
  Rat   100 KQEWIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFE-GASTKTVVRYNGTVTWTQP 163

  Fly   143 AIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWD 207
            |.|||.|.|||.:||||.|.|.||||||||||..||:    ...|.|     :|..|::.:.||:
  Rat   164 ANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDI----ILEDQD-----VDRTDFFDNGEWE 219

  Fly   208 IMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKI 272
            ||.....:..:..|||..||  |.::..::|..||||:.|||||:|:|||:|:||||||:.||||
  Rat   220 IMSAMGSKGNRTDSCCWYPY--ITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPSNEGEKI 282

  Fly   273 SLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHR- 336
            |||.|:|:|||||.|::.||||.:|..:||:|:||:|||:.||||::||:..:|::.||..||.
  Rat   283 SLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTHNA 347

  Fly   337 MAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRFSGDYGIPA 401
            |||||:::|:..||||||:.                       ...|::.....:..||
  Rat   348 MAPWVRKIFLHKLPKLLCMR-----------------------SHADRYFTQREEAESG------ 383

  Fly   402 LPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCPAAAAAAAADLS 466
                       ||                           |...|
  Rat   384 -----------AG---------------------------PKSRN-------------------- 390

  Fly   467 PTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALII 531
                     .:|..::..|:|.:||..::....|.||||::|.||||||||.|.:..::||  :.
  Rat   391 ---------TLEAALDCIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVSIIGT--LG 444

  Fly   532 LQAPSLY 538
            |..|.:|
  Rat   445 LFVPVIY 451

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	102/217 (47%)
		LIC	27..530	CDD:455710	202/503 (40%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
Chrna5	NP_058774.2	LGIC_ECD	45..251	CDD:475126	102/217 (47%)
		Neur_chan_memb	257..446	CDD:460753	96/286 (34%)