Protein Alignment nAChRalpha1 and Chrna3

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_434692.2
• Gene: Chrna3 / 25101
• RGDID: 2345
• Length: 499
• Species: Rattus norvegicus
• Alignment Length: 539
• Identity: 237/539 - (43%)
• Similarity: 339/539 - (62%)
• Gaps: 54/539 - (10%)

  Fly     1 MGSVL----FAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQL 61
            ||.||    .:.:.:.|.......|:....||:..|..:||.:||||.|.|..:.::..:.:|||
  Rat     1 MGVVLLPPPLSMLMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQL 65

  Fly    62 IDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIM 126
            :.|:..||||.||:|::|.||||||||.|.||.||:.:.||:|.||.|||||||||||:::|...
  Rat    66 VKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDK 130

  Fly   127 TKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNI 191
            |||:|.:||:|.|.||||:||.|:|||.|||||.|.|.||||||:||...:||           :
  Rat   131 TKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL-----------V 184

  Fly   192 EVG--IDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGI 254
            .:|  ::|:||:.|.||.|::.|..::|..|:||||.|.||.::|.:||..||||:||||||:.|
  Rat   185 LIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLI 249

  Fly   255 SFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVV 319
            |||:|||||||||.|||::||||:|||||||.|::.|.||.|||.:||:|:||||||:.||||:|
  Rat   250 SFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIV 314

  Fly   320 VTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDK 384
            :|:.||||::|:|.||.|..||:.:|:.:||:::.:.||...|.:   .|:..|.......:::.
  Rat   315 ITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSGEGD---TPKTRTFYGAELSNLNC 376

  Fly   385 FVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDI 449
            |...|||  |...|.|           ...|...:|.......|:          || :.|....
  Rat   377 FSRADSK--SCKEGYP-----------CQDGTCGYCHHRRVKISN----------FS-ANLTRSS 417

  Fly   450 SPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRM 514
            |.....|..:.:|  |||        |:::.|:..::||:::|.::..:.:::||||||||:||:
  Rat   418 SSESVDAVLSLSA--LSP--------EIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRI 472

  Fly   515 FLWIFAIACVVGTALIILQ 533
            |||:|.:.|::|||.:.||
  Rat   473 FLWVFILVCILGTAGLFLQ 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	109/219 (50%)
		LIC	27..530	CDD:455710	229/504 (45%)
		Cys-loop	149..163	CDD:349832	10/13 (77%)
Chrna3	NP_434692.2	LIC	24..490	CDD:455710	230/513 (45%)
		LGIC_ECD_nAChR_A3	29..235	CDD:349817	109/216 (50%)
		Cys-loop	153..167	CDD:349817	10/13 (77%)