Protein Alignment nAChRalpha1 and acr-21

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_001355512.1
• Gene: acr-21 / 185004
• WormBaseID: WBGene00000060
• Length: 635
• Species: Caenorhabditis elegans
• Alignment Length: 662
• Identity: 205/662 - (30%)
• Similarity: 312/662 - (47%)
• Gaps: 163/662 - (24%)

  Fly     8 AVFIALHFATGG-----LANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLK 67
            ::|:..:.|.||     .|:.:..|||.|||.:||..:||..::.:.:.|.....|:|:|||:.:
 Worm     9 SLFLFFNGAHGGNELSSRADQNVMRLYRDLLYDYNNEVRPSVHSKEPINVTFVFSLTQIIDVDER 73

  Fly    68 NQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIM-TKAIL 131
            |||:|||.|:...|.||||.|:|..|..|..:|:||:.||.|||:||||||..|..::| |..|:
 Worm    74 NQILTTNSWIRLHWVDYKLVWDPRLYQNVTRIHIPSDKIWKPDIILYNNADAQYMKSVMSTDVIV 138

  Fly   132 HHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGID 196
            .:.|.:.|...||:.|.|.:||:::|||.|||.:|:.||.|||..:||. ||.       |.| |
 Worm   139 DYLGNIHWPLSAIFTSSCPLDVKHYPFDRQTCILKYASWAYDGTKIDLL-LKS-------EQG-D 194

  Fly   197 LQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLV 261
            |.:|..:.||.::.:.|.:|:..||||.|||..|..::|:.|:.:||..|||:|||.||.::::.
 Worm   195 LTNYITNTEWSLIGIRAEKNQVIYSCCPEPYPFIDVHVTIERRAMFYVFNLILPCVLISLIALMG 259

  Fly   262 FYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLN 326
            ||:|:|||||::|.|:.|||.|||.:::||.:|||:..:||:|.|...|:|||.|...:|:..:|
 Worm   260 FYMPTDSGEKVTLGITSLLSTTVFLMMVAEGMPPTAEALPLIGIYFGVTIMLVALGTAMTVFTVN 324

  Fly   327 VNFRSPVTHRMAPWVQRLFIQILPKLLCI------------------ERP--------------- 358
            ::......:.:.|::|....:.|.|:|.:                  |.|
 Worm   325 IHHTGVHGYPVPPFLQIFAFRYLSKILFVRIEPYHSIAHHVRYMYQKEHPTECTLSASVHYKRLH 389

  Fly   359 -------KKEEP---EEDQPP-EVLTDVYHLPPDVDKFV-----------NYDSKR--------- 392
                   ||||.   :.|... .|::...|..|| |:.:           .:||::
 Worm   390 DFDANVAKKEEQYGLQRDMSNLSVISANVHCNPD-DRLLLGSQSSQKNLNKFDSEKNLRSSLSIR 453

  Fly   393 ---------------------------------FS--GDYGIP---------------ALPASHR 407
                                             ||  ..:|.|               ..|.|..
 Worm   454 SGSPTAKKKGHVRVSNIARSTRIMETDVSHLVSFSSLNSHGTPIDSPIYGRKLGRTQSQTPTSSY 518

  Fly   408 FDLAAAGGISAHCFAEPPLPSSLPLPG----AD-DDLFSPSGLNGDISPGCCPAAAAAAAADLSP 467
            .:....|||::     .|||..|..|.    .| ||:|..   |.:..|             :..
 Worm   519 RNSNGNGGITS-----DPLPQQLSNPPKISLTDVDDVFEN---NENQQP-------------MVD 562

  Fly   468 TFEKPYAREMEKT---IEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTAL 529
            .|||.:.|.|...   ||.:.   ..|..|||.:::..:|:.|||||||..|.:|.|...:.| .
 Worm   563 EFEKEFLRVMSMVHGIIERNE---MRVAEKDKRDAIALEWQQVAMVLDRFLLVVFLIGTSMST-F 623

  Fly   530 IILQAPSLYDQS 541
            :||...:|::.:
 Worm   624 VILNQRNLFEST 635

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	93/218 (43%)
		LIC	27..530	CDD:455710	197/625 (32%)
		Cys-loop	149..163	CDD:349832	7/13 (54%)
acr-21	NP_001355512.1	LGIC_ECD_nAChR	56..237	CDD:349798	82/189 (43%)
		Cys-loop	156..170	CDD:349798	7/13 (54%)
		LGIC_TM_cation	239..>327	CDD:349853	42/87 (48%)
		TM1 helix	239..263	CDD:349853	12/23 (52%)
		TM2 helix	271..292	CDD:349853	10/20 (50%)
		TM3 helix	303..325	CDD:349853	7/21 (33%)