Protein Alignment nAChRalpha1 and nAChRbeta1

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: XP_309158.3
• Gene: nAChRbeta1 / 1270461
• VectorBaseID: AGAMI1_011382
• Length: 519
• Species: Anopheles gambiae
• Alignment Length: 568
• Identity: 247/568 - (43%)
• Similarity: 338/568 - (59%)
• Gaps: 72/568 - (12%)

  Fly     3 SVLFAAVFIALHFATG-GLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNL 66
            |||..:|.:||....| |..:.|.:||..||...||:|||||.|.:.::.|:.||...|||:||.
Mosquito     4 SVLILSVLLALQQYLGMGSCSEDEERLVRDLFRGYNKLIRPVQNMTQKVDVRFGLAFVQLINVNE 68

  Fly    67 KNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAIL 131
            |||||.:|||:...|:||:|:|:..||||:..|.:|.:.:|.|||||:|||||||||...:..::
Mosquito    69 KNQIMKSNVWLRLVWSDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLI 133

  Fly   132 HHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGID 196
            :..|:|:|.|||||:|.|.|||.|||||:|||.|||||||::|..|.|    ...::.|.   :|
Mosquito   134 YPNGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSL----ALYNNKNF---VD 191

  Fly   197 LQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLV 261
            |.||:.|..|||:.|||..|....:..|   .||.|.:.:||||||||||||:|.|.||||.|||
Mosquito   192 LSDYWKSGTWDIIEVPAYLNVYEGNPTE---TDITFYIIIRRKTLFYTVNLILPTVLISFLCVLV 253

  Fly   262 FYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLN 326
            ||||:::|||::|.|||||||.||.||:::|:|||||.:||:.||||||.::.|:|::||:.::|
Mosquito   254 FYLPAEAGEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIIN 318

  Fly   327 VNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKK-------EEPEEDQPPEVLTDVYHLPPDVDK 384
            .|||.|.||||..|::.:|:..||.:|.::||:|       |.|....||:.     |..|
Mosquito   319 WNFRGPRTHRMPMWIRSVFLHYLPAMLLMKRPRKTRLRWMMEMPGMSVPPQP-----HTHP---- 374

  Fly   385 FVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPS-----G 444
                       .||.||....|   ::|.|       |:......:.|    .||..|:     .
Mosquito   375 -----------SYGSPAEIPKH---ISALG-------AKQSKMEVMEL----SDLHHPNCKMNRK 414

  Fly   445 LN-GDISPGCCPAAAAAAAAD---LSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWK 505
            || ||:..|.......:.::|   |||        |..|..|...|||:|::|:|.:....||||
Mosquito   415 LNSGDLGIGADSCRRESESSDSILLSP--------EASKATEAVEFIAEHLRNEDLYIQTREDWK 471

  Fly   506 YVAMVLDRMFLWIFAIACVVGTALIILQAPSLY---DQSQPIDILYSK 550
            |||||:||:.|:||.|....||..|::.||.::   ||.:.|:|...|
Mosquito   472 YVAMVIDRLQLYIFFIVTTAGTVGILMDAPHIFEYVDQDRIIEIYRGK 519

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	107/217 (49%)
		LIC	27..530	CDD:455710	230/518 (44%)
		Cys-loop	149..163	CDD:349832	10/13 (77%)
nAChRbeta1	XP_309158.3	LIC	6..498	CDD:455710	238/543 (44%)
		LGIC_ECD_nAChR_proto_beta-like	26..232	CDD:349833	105/215 (49%)
		Cys-loop	151..165	CDD:349833	10/13 (77%)