Protein Alignment nAChRalpha1 and Chrnb2

DIOPT Version :9

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_033732.2
• Gene: Chrnb2 / 11444
• MGIID: 87891
• Length: 501
• Species: Mus musculus
• Alignment Length: 546
• Identity: 237/546 - (43%)
• Similarity: 330/546 - (60%)
• Gaps: 90/546 - (16%)

  Fly     3 SVLFAAVFIALHFATGGLANPDAKRLYDDLL--SNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVN 65
            ::||:  |..|...:|.|.....:||.:.||  |.||:||||..|.|:.:||::.:.|:|||.|:
Mouse     9 ALLFS--FGLLWLCSGVLGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVH 71

  Fly    66 LKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAI 130
            .:.||||||||:.|||.||:|.|.|:|:..:..:.:||:||||||:||||||||.|||:..:.|:
Mouse    72 EREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAV 136

  Fly   131 LHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGI 195
            :.:.|.:.|.|||||||.|:|:|::||||:|.|.|||.|||||...:|| .||....|
Mouse   137 VSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL-VLKSDVAS------- 193

  Fly   196 DLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVL 260
             |.|:..|.||||:.:|..|||   :..:..|:||.::..:|||.||||:|||||||.|:.|::|
Mouse   194 -LDDFTPSGEWDIIALPGRRNE---NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAIL 254

  Fly   261 VFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVL 325
            |||||||.|||::||||:||:||||.||:::|:|||||.|||:||||:|||:|||.|:|.::.||
Mouse   255 VFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVL 319

  Fly   326 NVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPK----------KEEPEEDQPPEVLTDVYHLPP 380
            ||:.|||.||.|||||:.:|::.||.||.:::|:          :....|.:....|  .:...|
Mouse   320 NVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHRCARQRLRLRRRQREREGAGTL--FFREGP 382

  Fly   381 DVDK---FVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSP 442
            ..|.   |||               |||.:       |::....|||                :.
Mouse   383 AADPCTCFVN---------------PASMQ-------GLAGAFQAEP----------------AA 409

  Fly   443 SGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYV 507
            :||...:.|..|                     .:.:.::|.||||.|::::|..:||.||||||
Mouse   410 AGLGRSMGPCSC---------------------GLREAVDGVRFIADHMRSEDDDQSVREDWKYV 453

  Fly   508 AMVLDRMFLWIFAIACVVGTALIILQ 533
            |||:||:|||||...||.||..:.||
Mouse   454 AMVIDRLFLWIFVFVCVFGTIGMFLQ 479

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	Neur_chan_LBD	25..240	CDD:280998	104/216 (48%)
		LIC	27..530	CDD:273305	229/517 (44%)
		Neur_chan_memb	247..530	CDD:280999	119/295 (40%)
Chrnb2	NP_033732.2	LIC	29..478	CDD:273305	229/521 (44%)
		Neur_chan_LBD	29..234	CDD:280998	104/216 (48%)
		Neur_chan_memb	241..477	CDD:280999	119/296 (40%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	230	1.000	Domainoid score	I2435
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H595
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm11113
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	6.820