Protein Alignment nAChRalpha1 and Chrna4

DIOPT Version :9

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_056545.3
• Gene: Chrna4 / 11438
• MGIID: 87888
• Length: 629
• Species: Mus musculus
• Alignment Length: 619
• Identity: 260/619 - (42%)
• Similarity: 344/619 - (55%)
• Gaps: 105/619 - (16%)

  Fly     1 MGSVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVN 65
            :|:.|..|   :.|..|...|.   :||...|.|.||:..|||.|.||.:.|:.||.::|||||:
Mouse    21 LGTGLLPA---SSHIETRAHAE---ERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVD 79

  Fly    66 LKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAI 130
            .|||:|||||||:|||:||||:|:|.||..|.::.:|||.||.|||||||||||::.||.:|||.
Mouse    80 EKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAH 144

  Fly   131 LHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGI 195
            |.:.|:|.|.|||||||.|.|||.:||||:|.|.|||||||||...:||..:...         :
Mouse   145 LFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSR---------V 200

  Fly   196 DLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVL 260
            |..|::.|.||.|:......|.:.|.||.|.|.||.:...:||..||||:||||||:.||.|:||
Mouse   201 DQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVL 265

  Fly   261 VFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVL 325
            ||||||:.|||::||||:|||||||.||:.||||.|||.:||:|:||||||:.||||:|:|:.||
Mouse   266 VFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVL 330

  Fly   326 NVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPK--KEE------------------PEEDQPPE 370
            ||:.|||.||.|..||:|:|:.|:|:||.::||.  |:.                  ||.:..|.
Mouse   331 NVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPESEPG 395

  Fly   371 VLTDV----------------------------YHLPPD-----VDK---FVNYDSKRFSGDYGI 399
            :|.|:                            .|..||     |:|   ..:..|.......|.
Mouse   396 ILGDICNQGLSPAPTFCNRMDTAVETQPTCRSPSHKVPDLKTSEVEKASPCPSPGSCHPPNSSGA 460

  Fly   400 PAL-----------PASHRFDLAAAGGISA------HCFAE---PPLPSSLPLPGADDDLFSPSG 444
            |.|           |:|..   ||.|.|..      :|.::   ..|..|.|..........||.
Mouse   461 PVLIKARSLSVQHVPSSQE---AAEGSIRCRSRSIQYCVSQDGAASLTESKPTGSPASLKTRPSQ 522

  Fly   445 L--NGDISPGCC---------PAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFE 498
            |  :...||..|         |.....|....:|....|.:..:.:.:||.::||.|:|.:|...
Mouse   523 LPVSDQTSPCKCTCKEPSPVSPITVLKAGGTKAPPQHLPLSPALTRAVEGVQYIADHLKAEDTDF 587

  Fly   499 SVEEDWKYVAMVLDRMFLWIFAIACVVGTALIIL 532
            ||:|||||||||:||:|||:|.|.|::||..:.|
Mouse   588 SVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 621

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	Neur_chan_LBD	25..240	CDD:280998	111/214 (52%)
		LIC	27..530	CDD:273305	253/589 (43%)
		Neur_chan_memb	247..530	CDD:280999	137/369 (37%)
Chrna4	NP_056545.3	Neur_chan_LBD	39..245	CDD:280998	111/217 (51%)
		Neur_chan_memb	252..620	CDD:280999	137/370 (37%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	420..459		6/38 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	503..529		6/25 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	476	1.000	Inparanoid score	I1470
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8871
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.760