Protein Alignment nAChRalpha1 and CHRNA5

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_000736.2
• Gene: CHRNA5 / 1138
• HGNCID: 1959
• Length: 468
• Species: Homo sapiens
• Alignment Length: 529
• Identity: 210/529 - (39%)
• Similarity: 294/529 - (55%)
• Gaps: 115/529 - (21%)

  Fly    16 ATGGLANPD--AKR---LYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNV 75
            |..||:.|.  ||.   |..||..:|.|.:|||.:.:|::.:|.||.:|||:||:.|||:|||||
Human    33 AQRGLSEPSSIAKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNV 97

  Fly    76 WVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWK 140
            |::|||.|.||:||||||||:..:.|||:.:|.|||||::||||.:|.| .||.::.:.|.|.|.
Human    98 WLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFDNADGRFEGT-STKTVIRYNGTVTWT 161

  Fly   141 PPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVE 205
            |||.|||.|.|||.:||||.|.|.||||||||||..||:    ...|.|     :|.:|::.:.|
Human   162 PPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDI----ILEDQD-----VDKRDFFDNGE 217

  Fly   206 WDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGE 270
            |:|:.....:..:..|||..||  :.::..::|..||||:.|||||:|:|||:||||||||:.||
Human   218 WEIVSATGSKGNRTDSCCWYPY--VTYSFVIKRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEGE 280

  Fly   271 KISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTH 335
            ||.||.|:|:|||||.|::.||||.:|..:||:|:||:|||:.||||::||:..:|::.||..||
Human   281 KICLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTH 345

  Fly   336 R-MAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRFSGDYGI 399
            . |||.|:::|:..||||||:.                       ..||::.....:..||
Human   346 NAMAPLVRKIFLHTLPKLLCMR-----------------------SHVDRYFTQKEETESG---- 383

  Fly   400 PALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCPAAAAAAAAD 464
             :.|.|.|..|.||
Human   384 -SGPKSSRNTLEAA--------------------------------------------------- 396

  Fly   465 LSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTAL 529
                            ::..|:|.:|:..::....|.||||::|.||||||||.|....:||:
Human   397 ----------------LDSIRYITRHIMKENDVREVVEDWKFIAQVLDRMFLWTFLFVSIVGS-- 443

  Fly   530 IILQAPSLY 538
            :.|..|.:|
Human   444 LGLFVPVIY 452

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	103/222 (46%)
		LIC	27..530	CDD:455710	201/506 (40%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
CHRNA5	NP_000736.2	LGIC_ECD_nAChR_A5	45..251	CDD:349819	102/217 (47%)
		Cys-loop	170..184	CDD:349819	9/13 (69%)
		Neur_chan_memb	257..447	CDD:460753	96/286 (34%)