Protein Alignment nAChRalpha1 and CHRNA4

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_000735.1
• Gene: CHRNA4 / 1137
• HGNCID: 1958
• Length: 627
• Species: Homo sapiens
• Alignment Length: 601
• Identity: 255/601 - (42%)
• Similarity: 338/601 - (56%)
• Gaps: 113/601 - (18%)

  Fly    26 KRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNP 90
            :||...|.|.||:..|||.|.||.:.|:.||.::|||||:.|||:|||||||:|||:||||:|:|
Human    38 ERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 102

  Fly    91 DDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEY 155
            .||..|.::.:|||.||.|||||||||||::.||.:|||.|.|.|:|.|.|||||||.|.|||.:
Human   103 ADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTF 167

  Fly   156 FPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWDIMRVPAVRNEKFY 220
            ||||:|.|.|||||||||...:||         .|:...:|..|::.|.||.|:......|.:.|
Human   168 FPFDQQNCTMKFGSWTYDKAKIDL---------VNMHSRVDQLDFWESGEWVIVDAVGTYNTRKY 223

  Fly   221 SCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVF 285
            .||.|.|.||.:...:||..||||:||||||:.||.|:||||||||:.||||:||||:|||||||
Human   224 ECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVF 288

  Fly   286 FLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILP 350
            .||:.||||.|||.:||:|:||||||:.||||:|:|:.||||:.|||.||.|..||:|:|:.|:|
Human   289 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVP 353

  Fly   351 KLLCIERPK--KE--------------------EPEEDQPPEVLTDVYH---------------- 377
            :||.::||.  |:                    |||.:.|....|...|
Human   354 RLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEP 418

  Fly   378 ----------LPP----DVDKFVNYDS-------------------------------------- 390
                      |||    :.:|...:.|
Human   419 GPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVR 483

  Fly   391 -KRFSGDYGIPALPASHRFDLAAAGGISA---HCFAEPPLPSSLPLPGADDDLFSPSGLNGDISP 451
             :..|..|.:|...|:...|..|||.:::   |. ||.| |...|.|........||.::
Human   484 CRSRSIQYCVPRDDAAPEADGQAAGALASRNTHS-AELP-PPDQPSPCKCTCKKEPSSVS----- 541

  Fly   452 GCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFL 516
               |:|.....:..:|....|.:..:.:.:||.::||.|:|.:|...||:|||||||||:||:||
Human   542 ---PSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFL 603

  Fly   517 WIFAIACVVGTALIIL 532
            |:|.|.|::||..:.|
Human   604 WMFIIVCLLGTVGLFL 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	113/215 (53%)
		LIC	27..530	CDD:455710	254/596 (43%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
CHRNA4	NP_000735.1	LGIC_ECD	37..243	CDD:475126	113/213 (53%)
		Neur_chan_memb	250..618	CDD:460753	136/377 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	382..481		11/98 (11%)
		PRK14959	<392..496	CDD:184923	11/103 (11%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..561		18/74 (24%)