Protein Alignment nAChRalpha1 and Chrna5

DIOPT Version :9

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_001360830.1
• Gene: Chrna5 / 110835
• MGIID: 87889
• Length: 467
• Species: Mus musculus
• Alignment Length: 535
• Identity: 209/535 - (39%)
• Similarity: 292/535 - (54%)
• Gaps: 128/535 - (23%)

  Fly    16 ATGGL-----ANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNV 75
            |.|||     |......|:.||..:|.:.:|||.:.||::.:|.||.:|||:||:.|||:|||||
Mouse    33 ARGGLPELSSAAKHEDSLFRDLFEDYEKWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTNV 97

  Fly    76 WVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWK 140
            |::|||.|.||:||||||||:..:.|||:.:|:|||||::||||.:| ...||.::.:.|.|.|.
Mouse    98 WLKQEWIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFE-GASTKTVVRYNGTVTWT 161

  Fly   141 PPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVE 205
            .||.|||.|.|||.:||||.|.|.||||||||||..||:    ...|.|     :|..|::.:.|
Mouse   162 QPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDI----ILEDQD-----VDRTDFFDNGE 217

  Fly   206 WDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGE 270
            |:||.....:..:..|||..|.  |.::..::|..||||:.|||||:|:|||:|:||||||:.||
Mouse   218 WEIMSAMGSKGNRTDSCCWYPC--ITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPSNEGE 280

  Fly   271 KISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTH 335
            |||||.|:|:|||||.|::.||||.:|..:||:|:||:|||:.||||::||:..:|::.||..||
Mouse   281 KISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTH 345

  Fly   336 R-MAPWVQRLFIQILPKLLCIER------PKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRF 393
            . |||||:::|:..||||||:..      .::||.|:|
Mouse   346 NAMAPWVRKIFLHKLPKLLCMRSHADRYFTQREEAEKD--------------------------- 383

  Fly   394 SGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCPAAA 458
                                ||                          |...|
Mouse   384 --------------------GG--------------------------PKSRN------------ 390

  Fly   459 AAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIAC 523
                             .:|..::..|:|.:||..::....|.||||::|.||||||||.|.:..
Mouse   391 -----------------TLEAALDCIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVS 438

  Fly   524 VVGTALIILQAPSLY 538
            ::||  :.|..|.:|
Mouse   439 IIGT--LGLFVPVIY 451

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	Neur_chan_LBD	25..240	CDD:280998	100/214 (47%)
		LIC	27..530	CDD:273305	201/509 (39%)
		Neur_chan_memb	247..530	CDD:280999	97/289 (34%)
Chrna5	NP_001360830.1	LGIC_ECD	45..251	CDD:355788	100/217 (46%)
		Neur_chan_memb	257..445	CDD:367256	97/291 (33%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S4201
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	6.780