Protein Alignment nAChRalpha1 and Chrnb4

DIOPT Version :9

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_683746.1
• Gene: Chrnb4 / 108015
• MGIID: 87892
• Length: 495
• Species: Mus musculus
• Alignment Length: 554
• Identity: 235/554 - (42%)
• Similarity: 327/554 - (59%)
• Gaps: 82/554 - (14%)

  Fly     4 VLFAAVFIALHFATGGLANPDAKRLYDDLL--SNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNL 66
            :|..::|..|......|||.:.| |.||||  :.||.||||..::|..:::::.|.|||||.||.
Mouse     6 LLLVSLFALLQPGDCRLANAEEK-LMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNE 69

  Fly    67 KNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAIL 131
            :.|||||::|::|||.||:|.||...|.||:.|.:|::.:|||||||||||||.|||::.|..|:
Mouse    70 REQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIV 134

  Fly   132 HHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGID 196
            ...|.:.|.|||||||.|:|:|::||||:|.|.:||.|||||...:|:.....||..|
Mouse   135 RSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMD------- 192

  Fly   197 LQDYYISVEWDIMRVPAVRNEKFYSCCEEP-YLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVL 260
              |:..|.||||:.:|..|...    .::| |:|:.::..::||.||||:|||||||.|:.|::|
Mouse   193 --DFTPSGEWDIVALPGRRTVN----PQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAIL 251

  Fly   261 VFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVL 325
            |||||||.|||::||||:||:||.|.||:::|:|||||.:||:||||||||:|||.|:|.|:.||
Mouse   252 VFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVL 316

  Fly   326 NVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDS 390
            ||:.|||.||.||.||:..|:..||..|.::||..|..
Mouse   317 NVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVS--------------------------- 354

  Fly   391 KRFSGDYGIPA-LPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCC 454
                     || :|.|.:..|..|...|.....    ||            |||.|.|:......
Mouse   355 ---------PARVPHSSQLHLTTAEATSTSALG----PS------------SPSNLYGNSMYFVN 394

  Fly   455 PAAAAAAAADLSPTFEKP----------YAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAM 509
            |..|...:|..|.|...|          :.:::::.:||..|||||:::.|:.:||.||||:|||
Mouse   395 PVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAM 459

  Fly   510 VLDRMFLWIFAIACVVGTALIILQAPSLYDQSQP 543
            |:||:|||:|.|.|::||..:.|  |.|:....|
Mouse   460 VVDRLFLWVFVIVCILGTMGLFL--PPLFQIHAP 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	Neur_chan_LBD	25..240	CDD:280998	99/217 (46%)
		LIC	27..530	CDD:273305	224/516 (43%)
		Neur_chan_memb	247..530	CDD:280999	120/293 (41%)
Chrnb4	NP_683746.1	LIC	21..483	CDD:273305	229/529 (43%)
		Neur_chan_LBD	26..231	CDD:280998	99/218 (45%)
		Neur_chan_memb	238..481	CDD:280999	120/294 (41%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm11113
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.820