Protein Alignment nAChRalpha1 and chrnb1

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_001240739.1
• Gene: chrnb1 / 100006941
• ZFINID: ZDB-GENE-070821-4
• Length: 492
• Species: Danio rerio
• Alignment Length: 545
• Identity: 196/545 - (35%)
• Similarity: 285/545 - (52%)
• Gaps: 84/545 - (15%)

  Fly     4 VLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKN 68
            ::.......|...||.....|  :|...|.|.||..:||..|..:|:.|::|:.||..:.:|:|:
Zfish     7 IIITCCLCGLGAMTGATEMED--KLMKKLFSAYNSKVRPARNPEERVVVRIGMILSSFVSLNMKD 69

  Fly    69 QIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHH 133
            :.|.|.|.:..|||||:|.|||.||||||.|.:.|..:|||||||.||.||.:.|.:......:.
Zfish    70 EEMNTIVMMNLEWNDYRLSWNPKDYGGVDVLRISSGKVWLPDIVLINNNDGVFGVALQVHVQAYS 134

  Fly   134 TGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQ 198
            .|:|.|.|||:|||.|.:.|.|||||.|.|.|.|.|:|||...|||:|.......:..|:.:| .
Zfish   135 NGRVTWTPPALYKSSCGVKVTYFPFDWQNCTMVFRSYTYDSSEVDLQHALDKRGKEIKEIILD-T 198

  Fly   199 DYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFY 263
            .:..|.||.|....:.:|.:     |:.|.||.|.|.:.||.:||.||:::||:.|:.:::..||
Zfish   199 GFSESGEWHIRHKVSRKNVR-----EDLYEDITFYLIIERKPMFYVVNIVLPCILITIIAIFNFY 258

  Fly   264 LPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVN 328
            ||.|:|||:.|.|::||:||||.||||..||.|||.||::..||:.||:|||.||::::.|||::
Zfish   259 LPPDAGEKMGLSINVLLTLTVFLLLLANKIPETSLGVPIIVNYLMITMILVTFSVILSVVVLNLH 323

  Fly   329 FRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSKRF 393
            .|||.||:|..||:::||.:||..|.:.|||.|.|...:.|....::..:            .|.
Zfish   324 HRSPNTHQMPLWVRKIFIHMLPPYLGMLRPKVETPLFLEKPAKKENIQAI------------NRV 376

  Fly   394 SGDYGI--PA-----LPASHRF-------DLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSG 444
            :.:|.|  |:     .|..||:       ||.......:|....||
Zfish   377 ADEYFIRKPSNMNFLFPKPHRYQPDGQCTDLRKFIDGPSHYLTLPP------------------- 422

  Fly   445 LNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAM 509
                                           |::..:|...:||:.::.:..:|:::|||:||||
Zfish   423 -------------------------------ELKTAVEAITYIAEQLQAEKDYEALKEDWQYVAM 456

  Fly   510 VLDRMFLWIFAIACVVGTALIILQA 534
            |.||:|||||.|...:||..|...|
Zfish   457 VADRLFLWIFVIFTTLGTLGIFTDA 481

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	91/217 (42%)
		LIC	27..530	CDD:455710	190/516 (37%)
		Cys-loop	149..163	CDD:349832	8/13 (62%)
chrnb1	NP_001240739.1	LGIC_ECD	26..235	CDD:475126	90/216 (42%)
		Neur_chan_memb	242..478	CDD:460753	96/297 (32%)