DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsp68 and Hspa5

DIOPT Version :9

Sequence 1:	NP_524474.1	Gene:	Hsp68 / 42852	FlyBaseID:	FBgn0001230	Length:	635	Species:	Drosophila melanogaster
Sequence 2:	NP_001156906.1	Gene:	Hspa5 / 14828	MGIID:	95835	Length:	655	Species:	Mus musculus

Alignment Length:	624	Identity:	374/624 - (59%)
Similarity:	495/624 - (79%)	Gaps:	13/624 - (2%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 IGIDLGTTYSCVGVFQYGKVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMNPKNSVFDA 67
            :||||||||||||||:.|:|||||||||||.|||||||| :.|||||||||||:..||:|:||||
Mouse    32 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 96

  Fly    68 KRLIGRRFDDSKIQEDIKHWPFKVINDNGKPKISVEFKGA-NKCFSPEEISSMVLTKMKETAEAY 131
            ||||||.::|..:|:|||..||||:....||.|.|:..|. .|.|:|||||:|||||||||||||
Mouse    97 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 161

  Fly   132 LGTTVKDAVITVPAYFNDSQRQATKDAGAIAGINVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196
            ||..|..||:||||||||:|||||||||.|||:||:||||||||||:||||||. :||:|:|:||
Mouse   162 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKR-EGEKNILVFD 225

  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVNHFAEEFKRKYKKDLRSNPRALRRL 261
            |||||||||:||||.| :|||.:|.|||||||||||.|::.||.:.:|:|..||:|.:.||:::|
Mouse   226 LGGGTFDVSLLTIDNG-VFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKL 289

  Fly   262 RTAAERAKRTLSSSTEASLEIDALYEGHDFYSKVSRARFEELCGDLFRNTLEPVEKALKDAKMDK 326
            |...|:|||.|||..:|.:||::.:||.||...::||:||||..||||:|::||:|.|:|:.:.|
Mouse   290 RREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKK 354

  Fly   327 SQIHDIVLVGGSTRIPKVQNLLQNFFGGKTLNLSINPDEAVAYGAAIQAAILSGDKSSEIKDVLL 391
            |.|.:||||||||||||:|.|::.||.||..:..||||||||||||:||.:||||:  :..|::|
Mouse   355 SDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQ--DTGDLVL 417

  Fly   392 VDVAPLSLGIETAGGVMTKLIERNSRIPCKQSKTFTTYADNQPAVTIQVFEGERALTKDNNVLGT 456
            :||.||:|||||.||||||||.||:.:|.|:|:.|:|.:||||.|||:|:||||.|||||::|||
Mouse   418 LDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGT 482

  Fly   457 FDLTGVPPAPRGVPKIDVTFDLDANGILNVTAKEQGTGNAKNITIKNDKGRLSQADIDRMLSEAE 521
            |||||:||||||||:|:|||::|.||||.|||:::||||...|||.||:.||:..:|:||:::||
Mouse   483 FDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAE 547

  Fly   522 KYAEEDERHRQRIAARNQLETYLFGVKEA---AENGGDRISAADKSSIVERCSEAMKWLDSNTTA 583
            |:||||::.::||..||:||:|.:.:|..   .|..|.::|:.||.::.:...|.::||:|:..|
Mouse   548 KFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDA 612

  Fly   584 EKEEYEYKLKELEQFCSPIMTKMHKGGGDGQQAPNFGQQ 622
            :.|:::.|.||||:...||::|::..||    .|..|::
Mouse   613 DIEDFKAKKKELEEIVQPIISKLYGSGG----PPPTGEE 647

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsp68	NP_524474.1	PTZ00009	2..635	CDD:240227	374/624 (60%)
Hsp68	NP_524474.1	HSPA1-2_6-8-like_NBD	3..379	CDD:212675	247/376 (66%)
Hspa5	NP_001156906.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..81		40/48 (83%)
		HSP70	31..636	CDD:365808	370/607 (61%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	126..281		110/156 (71%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	410..420		2/11 (18%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	421..501		56/79 (71%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	632..655		5/20 (25%)
		Prevents secretion from ER	652..655
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.720