DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Atg6 and Becn1

DIOPT Version :9

Sequence 1:	NP_651209.1	Gene:	Atg6 / 42850	FlyBaseID:	FBgn0264325	Length:	422	Species:	Drosophila melanogaster
Sequence 2:	NP_062530.2	Gene:	Becn1 / 56208	MGIID:	1891828	Length:	448	Species:	Mus musculus

Alignment Length:	435	Identity:	220/435 - (50%)
Similarity:	301/435 - (69%)	Gaps:	26/435 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 VSFACQRCLQPIVLDEQLEKISVHAMAELSLPI----YGDNGNTLDPQDASSFD----------- 58
            |||.||||.||:.||...:.:....:.||:.|:    ....|.|.: ::|:|.:
Mouse    13 VSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQE-EEANSGEEPFIETRQDGV 76

  Fly    59 --HFVPPYRLTDSINGTGFMLV---SDGRDNKKMSAAFKLKAELFDCLSSNSEIDHPLCEECADS 118
              .|:||.|:..:.:...|.|:   |||...:.:|...|:..:|||.:|..:::||||||||.|:
Mouse    77 SRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDT 141

  Fly   119 MLEIMDRELRIAEDEWDVYKAYLDELEQQRVAPNVEALDKELDELKRSEQQLLSELKELKKEEQS 183
            :|:.:|.:|.:.|:|...||..|:.|||.. ..:.|.|.:||.||...|::|:.||::::|..:.
Mouse   142 LLDQLDTQLNVTENECQNYKRCLEILEQMN-EDDSEQLQRELKELALEEERLIQELEDVEKNRKV 205

  Fly   184 LNDAIAEEEQEREELHEQEESYWREYTKHRRELMLTEDDKRSLECQIAYSKQQLDKLRDTNIFNI 248
            :.:.:.:.:.|.|.|.::|..|.|||::.:|:.:..:|:.:|:|.|:.|::.|||||:.||:||.
Mouse   206 VAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQVRYAQIQLDKLKKTNVFNA 270

  Fly   249 TFHIWHAGHFGTINNFRLGRLPSVSVDWSEINAAWGQTVLLLSALARKIGLTFERYRVVPFGNHS 313
            ||||||:|.|||||||||||||||.|:|:|||||||||||||.|||.|:||.|:|||:||:||||
Mouse   271 TFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHS 335

  Fly   314 YVEVL-GENRELPLYGSGGFKFFWDTKFDAAMVAFLDCLTQFQKEVEKRDTEFLLPYKM--EKGK 375
            |:|.| .:::|||||.|||.:||||.|||.||||||||:.||::||||.:|.|.|||:|  ||||
Mouse   336 YLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGK 400

  Fly   376 IIDP-STGNSYSIKIQFNSEEQWTKALKFMLTNLKWGLAWVSSQF 419
            |.|. .:|.|||||.||||||||||||||||||||||||||||||
Mouse   401 IEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQF 445

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Atg6	NP_651209.1	APG6	110..418	CDD:282029	182/311 (59%)
Becn1	NP_062530.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	47..66		4/19 (21%)
		BH3	105..127	CDD:291932	6/21 (29%)
		BH3. /evidence=ECO:0000250\|UniProtKB:Q14457	106..125		5/18 (28%)
		Interaction with BCL2 and BCL2L1 isoform Bcl-X(L). /evidence=ECO:0000250\|UniProtKB:Q14457	110..157		19/46 (41%)
		APG6	133..444	CDD:282029	182/311 (59%)
		MT	194..>267	CDD:289543	23/72 (32%)
		Evolutionary conserved domain (ECD). /evidence=ECO:0000305\|PubMed:28445460	243..448		147/203 (72%)
		Required for membrane-association. /evidence=ECO:0000250\|UniProtKB:Q14457	423..448		23/23 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167836256
Domainoid	1	1.000	280	1.000	Domainoid score	I1673
eggNOG	1	0.900	-	-		E1_KOG2751
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2794
Inparanoid	1	1.050	426	1.000	Inparanoid score	I1742
Isobase	1	0.950	-	0	Normalized mean entropy	S2331
OMA	1	1.010	-	-		QHG49029
OrthoDB	1	1.010	-	-		D1085752at2759
OrthoFinder	1	1.000	-	-		FOG0002528
OrthoInspector	1	1.000	-	-		oto93471
orthoMCL	1	0.900	-	-		OOG6_102610
Panther	1	1.100	-	-	LDO	PTHR12768
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1774
SonicParanoid	1	1.000	-	-		X1898
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	17	16.750