DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Atg6 and becn1

DIOPT Version :9

Sequence 1:	NP_651209.1	Gene:	Atg6 / 42850	FlyBaseID:	FBgn0264325	Length:	422	Species:	Drosophila melanogaster
Sequence 2:	NP_957166.1	Gene:	becn1 / 393846	ZFINID:	ZDB-GENE-040426-1666	Length:	447	Species:	Danio rerio

Alignment Length:	438	Identity:	219/438 - (50%)
Similarity:	297/438 - (67%)	Gaps:	33/438 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 VSFACQRCLQPIVLDEQ---LEKISVHAMAELSLPIYGDNGN-TLDPQDASSFDH---------- 59
            |||.||||.||:.||..   |:::::|   ||:.|:.....| ..|..::|||..
Zfish    13 VSFVCQRCNQPLKLDTSFNVLDRMTIH---ELTAPLVMVTANKQQDSGESSSFPEETFLENKQDG 74

  Fly    60 ----FVPPYRLTDSINGTGFMLV---SDGRDNKKMSAAFKLKAELFDCLSSNSEIDHPLCEECAD 117
                |:||.|:..:.:...|.|:   |||...:.:|...|:.:.|||.:|..::|||||||||.|
Zfish    75 VARKFIPPARMMSAESTNSFTLIGEASDGGTMENLSRRLKVTSNLFDIMSGQTDIDHPLCEECTD 139

  Fly   118 SMLEIMDRELRIAEDEWDVYKAYLDELEQQRVAPNVE--ALDKELDELKRSEQQLLSELKELKKE 180
            ::|:.:|.:|.|.|:|...||:.|:.|.|   .|..|  :|...|.:||:.|:.|:.||:.::.:
Zfish   140 TLLDHLDTQLNITENECQNYKSCLELLSQ---LPEEEEASLLNALQQLKQEEESLIQELESIETK 201

  Fly   181 EQSLNDAIAEEEQEREELHEQEESYWREYTKHRRELMLTEDDKRSLECQIAYSKQQLDKLRDTNI 245
            .:::...:.|.....:.:..:|..|.:||.:.:|:.:..:||.:|::.|:.|.:.|||||:.||:
Zfish   202 REAVAKELDEGRNHSQLMDTEELRYQKEYCEFKRQQLELDDDLKSVDNQMRYCQIQLDKLKKTNV 266

  Fly   246 FNITFHIWHAGHFGTINNFRLGRLPSVSVDWSEINAAWGQTVLLLSALARKIGLTFERYRVVPFG 310
            ||.||||||:|.|||||||||||||||.|:|:|||||||||||||.|||.|:||.|:||::||:|
Zfish   267 FNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALASKMGLCFQRYQLVPYG 331

  Fly   311 NHSYVEVLGE-NRELPLYGSGGFKFFWDTKFDAAMVAFLDCLTQFQKEVEKRDTEFLLPYKM--E 372
            ||||:|.|.: ::|||||.|||.:||||.|||.||||||||:.||::||||.||.|.|||:|  :
Zfish   332 NHSYLESLSDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKDDTGFCLPYRMDVD 396

  Fly   373 KGKIIDP-STGNSYSIKIQFNSEEQWTKALKFMLTNLKWGLAWVSSQF 419
            ||||.|. .:|.|||||.||||||||||||||||||||||||||||||
Zfish   397 KGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQF 444

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Atg6	NP_651209.1	APG6	110..418	CDD:282029	177/313 (57%)
becn1	NP_957166.1	BH3	104..126	CDD:291932	6/21 (29%)
		BH3. /evidence=ECO:0000250\|UniProtKB:Q14457	105..124		5/18 (28%)
		Interaction with BCL2 and BCL2L1 isoform Bcl-X(L). /evidence=ECO:0000250\|UniProtKB:Q14457	109..156		21/46 (46%)
		APG6	132..442	CDD:309295	176/312 (56%)
		Evolutionary conserved domain (ECD). /evidence=ECO:0000305\|PubMed:28445460	242..447		146/203 (72%)
		Required for membrane-association. /evidence=ECO:0000250\|UniProtKB:Q14457	422..447		23/23 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170579719
Domainoid	1	1.000	279	1.000	Domainoid score	I1667
eggNOG	1	0.900	-	-		E1_KOG2751
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2794
Inparanoid	1	1.050	411	1.000	Inparanoid score	I1835
OMA	1	1.010	-	-		QHG49029
OrthoDB	1	1.010	-	-		D1085752at2759
OrthoFinder	1	1.000	-	-		FOG0002528
OrthoInspector	1	1.000	-	-		oto40239
orthoMCL	1	0.900	-	-		OOG6_102610
Panther	1	1.100	-	-	LDO	PTHR12768
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1774
SonicParanoid	1	1.000	-	-		X1898
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	17	16.800