Protein Alignment spas and SPAST

DIOPT Version :10

• Sequence 1: NP_651206.3
• Gene: spas / 42846
• FlyBaseID: FBgn0039141
• Length: 758
• Species: Drosophila melanogaster
• Sequence 2: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Alignment Length: 729
• Identity: 320/729 - (43%)
• Similarity: 404/729 - (55%)
• Gaps: 144/729 - (19%)

  Fly    56 GGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSF 120
            |.....|.|.:||...|...|         ..|.|  ..|.:|.    .....|.||:|||..|:
Human     6 GRGKKKGSGGASNPVPPRPPP---------PCLAP--APPAAGP----APPPESPHKRNLYYFSY 55

  Fly   121 PIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNRE 185
            |:...|.:||.:.:.|..:|.:|               |
Human    56 PLFVGFALLRLVAFHLGLLFVWL---------------C-------------------------- 79

  Fly   186 GDGQEQQLSNQPQRF-RPIQPLEMAANRPGGGY---------SPGPGDPLLAKQKHHHRRAFEYI 240
                        ||| |.:    |||.|..|..         :|.||.. ..:.:..|::|||||
Human    80 ------------QRFSRAL----MAAKRSSGAAPAPASASAPAPVPGGE-AERVRVFHKQAFEYI 127

  Fly   241 SKALKIDE-ENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDR 304
            |.||:||| |..|.||.|:|.|:|||:|||.|||| ..:|:|:..:||:||..||.|||.||:||
Human   128 SIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAV-IVTGQGEQCERARRLQAKMMTNLVMAKDR 191

  Fly   305 LHFLALREQDLQMQR-----------LSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSG 358
            |..|...:..|...:           |:.:....:.......|.::|:.....:.|....|..:|
Human   192 LQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTG 256

  Fly   359 YGPKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVR 423
            ....:.....|:.||..:..|.|:                 ||         .||.|.
Human   257 SAGLSGHHRAPSYSGLSMVSGVKQ-----------------GS---------GPAPTT------- 288

  Fly   424 RQFSSGRNTPPQRSRTPINNNGPSGSGAST----PVVSVKGVEQKLVQLILDEIVEGGAKVEWTD 484
                  ....|:.:||    |.||....:|    .:.:.:.|:..|..||::|||:.|..|::.|
Human   289 ------HKGTPKTNRT----NKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDD 343

  Fly   485 IAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISA 549
            |||||:|||||||:|||||:||||||||||||:|||||||||||||:||:|||.|.:|||.||||
Human   344 IAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISA 408

  Fly   550 ASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLP 614
            |||||||||:|||||||||||||.:||||||||||||||.||...||:||||||||||:||||:.
Human   409 ASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQ 473

  Fly   615 GNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRL 679
            ...| ||::|:.|||||||||||.||||.||||||||:|:||.|||..||.||||||..:.|.:|
Human   474 SAGD-DRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQL 537

  Fly   680 AKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLN 744
            |::||||||||||||||||||.|||||..||||.:..|.||.|...||..|||:|:|||:||:|.
Human   538 ARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLE 602

  Fly   745 SYEKWSQDYGDITI 758
            :|.:|::|:||.|:
Human   603 AYIRWNKDFGDTTV 616

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
spas	NP_651206.3	MIT_spastin	230..308	CDD:239142	45/78 (58%)
		RecA-like_spastin	484..648	CDD:410932	132/163 (81%)
		AAA_lid_3	676..727	CDD:465537	34/50 (68%)
		Vps4_C	<722..754	CDD:462762	16/31 (52%)
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		104/410 (25%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		83/261 (32%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80		26/141 (18%)
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50		14/58 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43		11/51 (22%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11		1/4 (25%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87		15/93 (16%)
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67		3/7 (43%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		45/85 (53%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		46/86 (53%)
		MIT_spastin	116..195	CDD:239142	45/79 (57%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		5/41 (12%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		233/431 (54%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		18/100 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		13/76 (17%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	132/163 (81%)
		AAA_lid_3	533..587	CDD:465537	36/53 (68%)
		Vps4_C	<580..612	CDD:462762	16/31 (52%)