DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spas and Spast

DIOPT Version :9

Sequence 1:	NP_651206.3	Gene:	spas / 42846	FlyBaseID:	FBgn0039141	Length:	758	Species:	Drosophila melanogaster
Sequence 2:	NP_001156342.1	Gene:	Spast / 50850	MGIID:	1858896	Length:	614	Species:	Mus musculus

Alignment Length:	769	Identity:	324/769 - (42%)
Similarity:	409/769 - (53%)	Gaps:	187/769 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    22 IKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGSSSNRRSPGSSPDGDDDTTTTD 86
            :.|.:|.....|..|...:..|....:.|.|..||.:.|||   |..:|:|.|
Mouse     1 MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAG---SPPKRNPSS------------ 50

  Fly    87 DLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLRSLIYQLFCIFRYLYGASTKVI 151
                               :||            |::..|.:||.|...|..:|.:|
Mouse    51 -------------------FSS------------PLVVGFALLRLLACHLGLLFAWL-------- 76

  Fly   152 YRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNREGDGQEQQLSNQPQRF-RPIQPLEMAANRPGG 215
                   |                                      ||| |.:    |||.|..|
Mouse    77 -------C--------------------------------------QRFSRAL----MAAKRSSG 92

  Fly   216 GY---------SPGPGDPLLAKQKHHHRRAFEYISKALKIDEENE-GHKELAIELYRKGIKELED 270
            ..         .||||.. ....:..|::||||||.||:||||.: |.||.|:|.|:|||:|||.
Mouse    93 TAPAPASPSPPEPGPGGE-AESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEK 156

  Fly   271 GIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLALREQDLQMQR-----------LSLKE 324
            |||| ..:|:|:.::||:||..||.|||.||:|||..|...:..||..:           |:.:.
Mouse   157 GIAV-IVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCRN 220

  Fly   325 KQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYGPKATTSAQPTASGRKLTIGSKRPVNLAVA 389
            ...:.......|.::|:.....:.|....|..||....:.....|:.||..:..|: ||
Mouse   221 GHLQSESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGA-RP------ 278

  Fly   390 NKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTP-PQRSRTPINNNGPSGSGAST 453
                                 .|...|.|          .:.|| |.|:..|           ||
Mouse   279 ---------------------GPGPAATT----------HKGTPKPNRTNKP-----------ST 301

  Fly   454 PVVSV---------KGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELF 509
            |..:|         :.|:..|..||::|||:.|..|::.|||||::|||||||:|||||:|||||
Mouse   302 PTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELF 366

  Fly   510 TGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHM 574
            |||||||:|||||||||||||:||:|||.|.:|||.|||||||||||||:|||||||||||||.:
Mouse   367 TGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVAREL 431

  Fly   575 QPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAAL 639
            ||||||||||||||.||...||:||||||||||:||||:....| ||::|:.|||||||||||.|
Mouse   432 QPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGD-DRVLVMGATNRPQELDEAVL 495

  Fly   640 RRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIR 704
            |||.||||||||:|:||.|||..||.||||||..:.|.:||::||||||||||||||||||.|||
Mouse   496 RRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIR 560

  Fly   705 ELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI 758
            ||..||||.:..|.||.|...||..|||:|:|||:||:|.:|.:|::|:||.|:
Mouse   561 ELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV 614

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spas	NP_651206.3	MIT_spastin	230..308	CDD:239142	45/78 (58%)
		P-loop_NTPase	482..>538	CDD:304359	46/55 (84%)
		AAA	515..652	CDD:214640	108/136 (79%)
		AAA	519..650	CDD:278434	103/130 (79%)
		Vps4_C	<722..754	CDD:286426	16/31 (52%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..298		108/439 (25%)
		Required for midbody localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..192		85/295 (29%)
		Required for interaction with ATL1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..78		27/175 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..48		14/49 (29%)
		Required for nuclear localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..48		14/49 (29%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	4..11		1/6 (17%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	48..85		17/136 (13%)
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	57..65		3/7 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	91..112		5/21 (24%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	110..194		45/85 (53%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	112..198		46/86 (53%)
		MIT_spastin	114..193	CDD:239142	45/79 (57%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..306		23/134 (17%)
		Sufficient for microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	226..614		234/437 (54%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	268..326		19/106 (18%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	307..310		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		P-loop_NTPase	339..>395	CDD:304359	46/55 (84%)
		AAA	372..508	CDD:214640	108/136 (79%)
		AAA	376..506	CDD:278434	103/130 (79%)
		Vps4_C	<578..610	CDD:286426	16/31 (52%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167836734
Domainoid	1	1.000	202	1.000	Domainoid score	I2983
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8970
Inparanoid	1	1.050	482	1.000	Inparanoid score	I1442
Isobase	1	0.950	-	0	Normalized mean entropy	S1340
OMA	1	1.010	-	-		QHG48730
OrthoDB	1	1.010	-	-		D1176820at2759
OrthoFinder	1	1.000	-	-		FOG0001088
OrthoInspector	1	1.000	-	-		oto94563
orthoMCL	1	0.900	-	-		OOG6_100722
Panther	1	1.100	-	-	LDO	PTHR23074
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2807
SonicParanoid	1	1.000	-	-		X3342
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	17	16.750