DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spas and spast

DIOPT Version :9

Sequence 1:	NP_651206.3	Gene:	spas / 42846	FlyBaseID:	FBgn0039141	Length:	758	Species:	Drosophila melanogaster
Sequence 2:	NP_998080.2	Gene:	spast / 405851	ZFINID:	ZDB-GENE-040426-2331	Length:	570	Species:	Danio rerio

Alignment Length:	582	Identity:	290/582 - (49%)
Similarity:	367/582 - (63%)	Gaps:	91/582 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly   200 FRPIQPLEMAANR----PGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIEL 260
            |:||: ..|||..    |.|....|      .:.:::|::|||:||.||:|||:.:|.|:.|::.
Zfish    54 FQPIR-RAMAARAKECGPDGSEETG------ERIRNYHKQAFEFISVALQIDEDEKGDKQKAVQW 111

  Fly   261 YRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLALREQDLQMQRLSLKEK 325
            |||||.|||.||.:.. :|.|:..|||::|.|||.||||||.|||..|.        ..||
Zfish   112 YRKGIAELEKGIQIQV-TGAGEKADRARKLQDKMITNLSMAEDRLKLLG--------NLLS---- 163

  Fly   326 QKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYGPKATTSAQPTASGRKLTIGSKRPVNLAVAN 390
                 ||..:.:.:......:|..::                ...|||..    ||:...|.:.|
Zfish   164 -----QSPAESSSDDSFYSFSNGNLR----------------PAPASGAV----SKKKDTLTITN 203

  Fly   391 KSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPP---QRSRTPINNN--GPSGSG 450
            ::...|:|             |.|  :||.|      ||.|..|   |.|||...||  ||:..|
Zfish   204 QTSLRPKN-------------PPK--STPNA------SGLNCTPSAAQSSRTGPQNNQKGPTVKG 247

  Fly   451 -----ASTPVV----------SVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVI 500
                 |||...          :.|.|:.||..|||:|||:.|:.|.:.||||||:|||||||:||
Zfish   248 KNNVKASTTATASPQRKRDMKNFKNVDSKLASLILNEIVDSGSVVRFDDIAGQDLAKQALQEIVI 312

  Fly   501 LPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVR 565
            ||::||||||||||||:|||||||||||||:||:|||.|.:|||.|||||:|||||||:||||||
Zfish   313 LPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAMESNATFFNISAATLTSKYVGEGEKLVR 377

  Fly   566 ALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNR 630
            |||||||.:|||||||||:||||.||...||:||||||||||:||||:....| :|::|:.||||
Zfish   378 ALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSGGD-ERVLVMGATNR 441

  Fly   631 PQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALA 695
            |||||||.||||.||:||:||.|:||..||..||.|..:||..:.|.:||::||||||||||:||
Zfish   442 PQELDEAVLRRFAKRIYVALPTEETRLKLLKNLLSKHRNPLSQKELSQLARLTDGYSGSDLTSLA 506

  Fly   696 KDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDIT 757
            |||||.|||||..|||:.:....||.|...||..|||||:|||:||:|:.|.:|:::|||.|
Zfish   507 KDAALGPIRELKPEQVRNMSAHEMRDIRISDFLESLKRIKRSVSPQTLDQYVRWNREYGDTT 568

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spas	NP_651206.3	MIT_spastin	230..308	CDD:239142	42/77 (55%)
		P-loop_NTPase	482..>538	CDD:304359	46/55 (84%)
		AAA	515..652	CDD:214640	103/136 (76%)
		AAA	519..650	CDD:278434	99/130 (76%)
		Vps4_C	<722..754	CDD:286426	16/31 (52%)
spast	NP_998080.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	186..269		30/107 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580222
Domainoid	1	1.000	198	1.000	Domainoid score	I3041
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8970
Inparanoid	1	1.050	471	1.000	Inparanoid score	I1471
OMA	1	1.010	-	-		QHG48730
OrthoDB	1	1.010	-	-		D1176820at2759
OrthoFinder	1	1.000	-	-		FOG0001088
OrthoInspector	1	1.000	-	-		oto41040
orthoMCL	1	0.900	-	-		OOG6_100722
Panther	1	1.100	-	-	LDO	PTHR23074
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2807
SonicParanoid	1	1.000	-	-		X3342
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	1	1.500	-	-
	18	18.300