DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spas and SPAST_ANOGA

DIOPT Version :9

Sequence 1:	NP_651206.3	Gene:	spas / 42846	FlyBaseID:	FBgn0039141	Length:	758	Species:	Drosophila melanogaster
Sequence 2:	XP_312634.5	Gene:	SPAST_ANOGA / 1273633	VectorBaseID:	AGAP002334	Length:	827	Species:	Anopheles gambiae

Alignment Length:	869	Identity:	468/869 - (53%)
Similarity:	554/869 - (63%)	Gaps:	153/869 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGS 65
            |||.| .:.:::..|.|.||...|.|....:.|...|...|...|.:       |.||...||..
Mosquito     1 MVRNK-YTLTTAGKSPSKKSRTGSLSKQHDATGDDDGETGTLDGSGS-------AAGSPVGGGTD 57

  Fly    66 SSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLR 130
            ::.:|..|                                  .||||||||::|||:||:|||||
Mosquito    58 AAAKRCDG----------------------------------GSVHKQNLYIISFPVIFVFNVLR 88

  Fly   131 SLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVV-------------QNSS------------- 169
            ||:||||.:|||:|..:|||:|||.|::|.:|||:             ::||
Mosquito    89 SLLYQLFIVFRYVYNFTTKVVYRPVRKECGLEIVINTDQHGHHHHHHHRHSSHSIHSTAAAHQLQ 153

  Fly   170 KEQQQSLNHPSELNREGDGQEQQLSNQPQRFRPIQPL--------------EMAANRPGGGYSPG 220
            :.|||..:..|.|.:|..|..:....|.|:.:...||              ||:..|...|...|
Mosquito   154 QHQQQQQHQYSLLQQEQHGVTEPQQQQQQQHQAAHPLQCSQSGILVNGEGREMSIQRSASGSQVG 218

  Fly   221 PGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWD 285
            ||||||||||||||||||||||||||||:||..||||||||||||.|||.||||:||.|||:||:
Mosquito   219 PGDPLLAKQKHHHRRAFEYISKALKIDEDNEDQKELAIELYRKGILELERGIAVECWGGRGEVWE 283

  Fly   286 RAQRLHDKMQTNLSMARDRLHFL-------ALREQDLQMQRLSLKEKQ-KEEAQSKPQKTR---- 338
            ||||||||||||||||||||.||       :::.:.|...|.:|..:. |.:..:.|.:.|
Mosquito   284 RAQRLHDKMQTNLSMARDRLQFLGMLCVCVSVQRKHLHPTRTTLPYRDVKSKLHTTPTENRMVSF 348

  Fly   339 -EPMLAGMTNEPMKLRVRSSGYGPKATTSAQPT---------ASGRKLTIGSKRPVNLAVANKSQ 393
             |.......:.|.:....||.....||..|.|:         |||||||:|.|||.||.|.||||
Mosquito   349 NENCTITTNHHPARGTAASSRPTTAATAPATPSLPIKQTASEASGRKLTVGYKRPGNLGVMNKSQ 413

  Fly   394 TLPRNL-GSKTS------------------------VGAVQRQP---AKTAATPPAVRRQFS--- 427
            ||||:: |::|:                        ||.....|   .|.||||||:|||||
Mosquito   414 TLPRSMGGTRTTPTGTGGAGGIAGGLGNGGTYGGSIVGGGAGMPKIVPKPAATPPAIRRQFSVSI 478

  Fly   428 ---------------SGRNTPPQRSRTPI---NNNGPSGSGASTPVVSVKGVEQKLVQLILDEIV 474
                           ..:||||.||:||:   ....|.......|.:||||||.||||:|:||||
Mosquito   479 PIPGSSPVRKASNGYGSKNTPPPRSKTPLAGQQPQQPQQQQQQQPQISVKGVEPKLVQIIMDEIV 543

  Fly   475 EGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATE 539
            ||||||:|.|||||:||||||||||||||||||||||||.|||||||||||||||||||||||||
Mosquito   544 EGGAKVQWQDIAGQEVAKQALQEMVILPSVRPELFTGLRTPAKGLLLFGPPGNGKTLLARAVATE 608

  Fly   540 CSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKT 604
            |||||.:||||:|||||||||||||||||||||.:||||||||||||:||||||:||||:|||||
Mosquito   609 CSATFFSISAATLTSKYVGDGEKLVRALFAVARELQPSIIFIDEVDSVLSERSSNEHEATRRLKT 673

  Fly   605 EFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGS 669
            ||||:|||||.|.:.|||||:|||||||||||||||||.|||||:|||..||||||.||||||||
Mosquito   674 EFLVQFDGLPANSEADRIVVMAATNRPQELDEAALRRFPKRVYVTLPDRDTRELLLRRLLQKQGS 738

  Fly   670 PLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRI 734
            ||....|..||::|:|||||||||||:|||||||||||||:||.:|.:.:|:|.|.|||:|||||
Mosquito   739 PLSDADLAHLAQLTEGYSGSDLTALARDAALEPIRELNVEEVKNMDPTKLRSIRESDFHNSLKRI 803

  Fly   735 RRSVAPQSLNSYEKWSQDYGDITI 758
            |||||||||.:||||.||:||:|:
Mosquito   804 RRSVAPQSLAAYEKWLQDFGDVTL 827

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spas	NP_651206.3	MIT_spastin	230..308	CDD:239142	67/77 (87%)
		P-loop_NTPase	482..>538	CDD:304359	52/55 (95%)
		AAA	515..652	CDD:214640	120/136 (88%)
		AAA	519..650	CDD:278434	115/130 (88%)
		Vps4_C	<722..754	CDD:286426	25/31 (81%)
SPAST_ANOGA	XP_312634.5	MIT_spastin	228..306	CDD:239142	67/77 (87%)
		P-loop_NTPase	551..>607	CDD:304359	52/55 (95%)
		AAA	584..721	CDD:214640	120/136 (88%)
		AAA	588..719	CDD:278434	115/130 (88%)
		Vps4_C	<794..823	CDD:286426	23/28 (82%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	419	1.000	Domainoid score	I1945
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8970
Inparanoid	1	1.050	798	1.000	Inparanoid score	I1443
OMA	1	1.010	-	-		QHG48730
OrthoDB	1	1.010	-	-		D1176820at2759
OrthoFinder	1	1.000	-	-		FOG0001088
OrthoInspector	1	1.000	-	-		oto110156
Panther	1	1.100	-	-	LDO	PTHR23074
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3342
TreeFam	1	0.960	-	-
	13	12.940