Protein Alignment eIF4G2 and tif471

DIOPT Version :9

• Sequence 1: NP_001287489.1
• Gene: eIF4G2 / 42819
• FlyBaseID: FBgn0260634
• Length: 2072
• Species: Drosophila melanogaster
• Sequence 2: NP_594602.1
• Gene: tif471 / 2542290
• PomBaseID: SPAC17C9.03
• Length: 1403
• Species: Schizosaccharomyces pombe
• Alignment Length: 1690
• Identity: 359/1690 - (21%)
• Similarity: 571/1690 - (33%)
• Gaps: 491/1690 - (29%)

  Fly   175 PTLPPHFQQSAGLIAPAGGNAGGPYISATYIPINQTGPLMGMQPAVTFPTNGAAGASFYAAAPPP 239
            |:..|.|..:..|.:.....:.....|...   ..|.....::|:...|||.:...:
pombe     6 PSNTPKFSYARALASSQSNKSNSTKASENN---TATAEKQAVKPSGVEPTNTSRANA-------- 59

  Fly   240 PQQKQLPPTAQQQGPGGAPGAAQTPTLAYYQLGPNAQNSAGNNVVSGANTHRGNMRGGQRSTPTA 304
              ||:...|      |.....|.|......:...|.:.|........::|:....||...|.|:|
pombe    60 --QKKTEST------GKITSEADTEKYNSSKSPVNKEGSVEKKSSEKSSTNNKPWRGDNTSKPSA 116

  Fly   305 QHTNYFTAYTMAPGNAQRPAPTNVAPMMNSRSGALNGAAAFHHLPHNFGIPSLALGPPAGMTTPI 369
            .            .:|:|.:..:..|..:|:.|..| ||...::  |....|....||.. |.||
pombe   117 N------------SSAERTSSQHQKPETSSQIGKDN-AAPVENV--NEKSTSQETAPPVS-TVPI 165

  Fly   370 LAPAGMQAPPPQPPGGLHTFAPHLQSNSAT---TLPSVPSVTGVPAVASGFSTTSLPTPSVTTAP 431
                                    |..|.|   .:||.|.|:|.....||.|:.|..:.||.::
pombe   166 ------------------------QFGSITRNAAIPSKPKVSGNMQNKSGVSSYSSKSQSVNSS- 205

  Fly   432 QLGANSDKVCKRKYAIPIIHPDTMENVMDGWKRSVFIKKDSNSTLTQHTDLDTALDDNSTLCLYQ 496
             :.:|.              |.|.|        .|..|.:::||.|:.                .
pombe   206 -VTSNP--------------PHTEE--------PVAAKPEASSTATKG----------------P 231

  Fly   497 APTLMDSGSGSSLLSGAMVDQ---QMQTVKPASSLKQSVSLEKVPEAVYCKDAVGQDVHPSPPPK 558
            .||...|.:.:|..:||..::   .:.|..||:...| ||....|.      ..|.....:...:
pombe   232 RPTTSASNTNTSPANGAPTNKPSTDINTTDPATQTTQ-VSASNSPA------LSGSSTPSNTSSR 289

  Fly   559 STDKDQISVIVKDILAHSGNAEDHLSFWQYSDVDNVTETHNS--------LPLVQSDLPDTTDTC 615
            |..::..:...|......||:  |.|:.:||...:....:||        .|..::...:..:..
pombe   290 SNRQNHGNFSEKRHYDRYGNS--HPSYNKYSHYQHGFNYNNSGNNRNESGHPRFRNSRRNYNNQG 352

  Fly   616 QQPVLFQ-----EQAKKQRPKLANKQST--ATPASIANDVAEAAASGCPGVSILSRGQSMVTP-- 671
            ..|....     .|:.:..|:..|..||  ..|.|:..          |...:..:.|.:|.|
pombe   353 AYPTYMSNGRSANQSPRNNPQNVNNGSTPIQIPVSLQT----------PYGQVYGQPQYIVDPNM 407

  Fly   672 --AGP---------------STP-----PPPSRVAQQKSTKSIVTTAVAASVKPTTG-----SPN 709
              .||               .||     |..||...|.|.: :|.:..::::.|..|     ...
pombe   408 VQYGPILQPGYVPQYYPVYHQTPYTQNFPNMSRSGSQVSDQ-VVESPNSSTLSPRNGFAPIVKQQ 471

  Fly   710 RKSS--------------IQAATSTTTSSGASSSTASTPTHASSKLKQQPVNNLQHGKQ--IPGK 758
            :|||              :....:::.:...::|.|.|||.|..::.::..:..:...:  |..:
pombe   472 KKSSALKIVNPVTHTEVVVPQKNASSPNPSETNSRAETPTAAPPQISEEEASQRKDAIKLAIQQR 536

  Fly   759 VSTPALPPTASRISAAVTLLAGTANITTTTNSSKKQRQAQKRARELEKKKQKSSITSTTSTQGQN 823
            :...| ...|.|.:.....|....|.........|:...:|..||.|:|.::.:          .
pombe   537 IQEKA-EAEAKRKAEEKARLEAEENAKREAEEQAKREAEEKAKREAEEKAKREA----------E 590

  Fly   824 NNGKGNGNGNAALEINTNSNNAATVTTTSMSTLTTTTTATAPTTKQETAAVDDKETAQPTTDTLS 888
            ...|.....||..|....:...|                      :|.|..:.:|.|:...
pombe   591 EKAKREAEENAKREAEEKAKREA----------------------EEKAKREAEEKAKREA---- 629

  Fly   889 ATSNNTKVSSILRDKKTMLNPKEQLTSHNNNNNNNNEDGIEAKVESDPESDIKDDSMSNITGEKS 953
                         ::|.....:|:....           .|.|.:.:.|...|.::..|...|..
pombe   630 -------------EEKAKREAEEKAKRE-----------AEEKAKREAEEKAKREAEENAKREAE 670

  Fly   954 EKHLANEEIVAKFLQKDSPDTSVVHAETQQLQFLNSSSSVCEEDEPVPVISEGVTKTEENAEAEI 1018
            ||.....|..||                            .|.:|.|.      .:|||||:.:.
pombe   671 EKAKREAEENAK----------------------------REAEEKVK------RETEENAKRKA 701

  Fly  1019 KFGDFHEDPRNDTGFICSSTWSSSTISKAAEDDSSQSSRSADNVDCAPKVVEQISEEKSASSTKS 1083
            :.....|..:|..  |.||...:|  |:|..|.|.|::.:...|....||.:..:.|..::|:.|
pombe   702 EEEGKREADKNPE--IKSSAPLAS--SEANVDTSKQTNATEPEVVDKTKVEKLKASEGKSTSSLS 762

  Fly  1084 SPVHKATG-----SGPGS------PKTKE-----LKSK---------SPSPIPVPQVPK-----N 1118
            ||.|..:.     ||..|      ||:::     |.|:         .||.|..|...:     .
pombe   763 SPSHSTSSKRDLLSGLESLSLKTNPKSEQCLESLLNSQFITDFSALVYPSTIKPPSTEEALKAGK 827

  Fly  1119 YRYSIETLREL--------AKRRDSRKPPMVPCQKGDCISQLFVSRQQQPQQHHGQHIQQYQHMS 1175
            |.|.:..|.:.        .|..|.|....|.....|..|:...|..:| ....|.:...:....
pombe   828 YEYDVPFLLQFQSVYTDKPMKGWDERMKETVASAFSDKSSRGMYSSSRQ-SSRSGSNTHSHAGPG 891

  Fly  1176 FNESLDYVPGKR-----------------GRGHGPNKK--------HHDQHQQMGGSSGMGSGSG 1215
            |..     |.:|                 |.|.|.|.|        ||......|...|...||.
pombe   892 FGG-----PSERKGISRLGIDRGFSSSGAGFGSGSNYKSAPSRGVSHHGHGGMSGSHRGSQRGSR 951

  Fly  1216 GGGGISNSSQRHMDIIRVQLSLKEEIKLSECENAWQPGTL-RRVSMAGGQDEDD--DVEGVLKKV 1277
            .|||    .:...|...:.:.:.:...|....|.|||..| .:.:...|:||:.  ..|.|.:||
pombe   952 RGGG----ERDKPDPSSLTIPVDQVAPLQLSANRWQPKKLTEKPAETKGEDEEALLPPEVVQRKV 1012

  Fly  1278 RGILNKLTPDNFEVLLKEMSSIKM----DNEAK-MTNVMLLIFEKTISEPNFAPTYARFCKVLFH 1337
            :|.|||:|.:.|:.:..::..|.|    :|:.: :..|:.|.|||...||||:..||||.:.:..
pombe  1013 KGSLNKMTLEKFDKISDQILEIAMQSRKENDGRTLKQVIQLTFEKATDEPNFSNMYARFARKMMD 1077

  Fly  1338 EIKAENKS---------------LFTSSLITRIQHEFESNVNDANAKSKKLQPTMERINQCSDPA 1387
            .|....:.               ||...|::|.|.:||.... ||..|.|              |
pombe  1078 SIDDSIRDEGVLDKNNQPVRGGLLFRKYLLSRCQEDFERGWK-ANLPSGK--------------A 1127

  Fly  1388 KKAELRAEMEDLEYQFRRRAWGTVRFIGELFKLQSLTNDRVLNCVESLLEHGC---EEKLEYMCK 1449
            .:||:.::...:....:||..|.||||||||||..|:...:..|::.||.:..   ||::|.:|:
pombe  1128 GEAEIMSDEYYVAAAIKRRGLGLVRFIGELFKLSMLSEKIMHECIKRLLGNVTDPEEEEIESLCR 1192

  Fly  1450 LLTTVGHLLESSLPEH-----YQLR-DRIEKIFRRIQDIINRSRGTSHRQQVHIKISSRVRFMMQ 1508
            ||.|||..::::...|     |.|| :.|.||                     ..:.||::||:.
pombe  1193 LLMTVGVNIDATEKGHAAMDVYVLRMETITKI---------------------PNLPSRIKFMLM 1236

  Fly  1509 DVLDLRLRNWDQPATHQSGQQGGR--GQRHKQHDDSK----DQSHSTGGGRGGGMNQHQQSSTHH 1567
            ||:|.|...|   |.....::|.:  .:.|::.:..|    .|..|:|...|..||:..
pombe  1237 DVMDSRKNGW---AVKNEVEKGPKTIAEIHEEAERKKALAESQRPSSGRMHGRDMNRGD------ 1292

  Fly  1568 QHQKHNQHGHDGGNYFMQKMPNKQHHENQTLSIDPSKLRFSNS--SATDDSIAKLGNSSHYQWRN 1630
                 ::.|..|.|                    |.   ||:|  |...|..|:||.
pombe  1293 -----SRMGGRGSN--------------------PP---FSSSDWSNNKDGYARLGQ-------- 1321

  Fly  1631 VGNRPV-SSTQVNASNAPPP-TSLLKRPGQNYKFLPYQQPSAV--AGTANAPAGGNKSNVEVNDA 1691
             |.|.: |.||  .|:.|.. :|:||  |.:....|.:|.||:  ..:..||    .|||.|..|
pombe  1322 -GIRGLKSGTQ--GSHGPTSLSSMLK--GGSVSRTPSRQNSALRREQSVRAP----PSNVAVTSA 1377

  Fly  1692  1691
            pombe  1378  1377

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF4G2	NP_001287489.1	EIN3	<270..423	CDD:296674	35/155 (23%)
		MIF4G	1274..1514	CDD:280935	79/268 (29%)
		MA3	1697..1812	CDD:280933
tif471	NP_594602.1	ASFV_J13L	<213..>284	CDD:114299	21/101 (21%)
		tolA_full	470..>574	CDD:274303	16/104 (15%)
		FKBP_N	<515..564	CDD:279662	6/49 (12%)
		eIF_4G1	784..846	CDD:288966	11/61 (18%)
		MIF4G	1009..1244	CDD:280935	80/270 (30%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0401
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm47287
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR23253
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.910