Protein Alignment PolE1 and polB

DIOPT Version :10

• Sequence 1: NP_524462.2
• Gene: PolE1 / 42758
• FlyBaseID: FBgn0264326
• Length: 2236
• Species: Drosophila melanogaster
• Sequence 2: NP_414602.1
• Gene: polB / 944779
• ECOCYCID: EG10747
• Length: 783
• Species: Escherichia coli
• Alignment Length: 1094
• Identity: 188/1094 - (17%)
• Similarity: 326/1094 - (29%)
• Gaps: 418/1094 - (38%)

  Fly    83 FFIQMDGSRFKCTVAYQPYLLIRPEDNMHLEVARFLGRKYSGQISGLEHITKEDLDL---PNHLS 144
            |::..|....:.|:|.|..:...|.|                |:...:||.:.:...   |..|.
E. coli    23 FWLATDNGPLQVTLAPQESVAFIPAD----------------QVPRAQHILQGEQGFRLTPLALK 71

  Fly   145 GLQQQYIKLSFLNQTAMTKVRRELMSAVKRNQER---------QKSNTYYMQMLATSLAQSSAGS 200
            ...:|.:      .....:..|:||:..||.:|.         :....|.|:...||........
E. coli    72 DFHRQPV------YGLYCRAHRQLMNYEKRLREGGVTVYEADVRPPERYLMERFITSPVWVEGDM 130

  Fly   201 EDATLGKRQQDYMDCIVDIREHDVPYHVRVSIDLRIFCGQWYNIRCRSGVELPTITCRPDILDRP 265
            .:.|           ||:.|                                  :...||.  ||
E. coli   131 HNGT-----------IVNAR----------------------------------LKPHPDY--RP 148

  Fly   266 EPVVLAFDIETTKLPLKFPDAQTDQVMMISYMIDGQG----YLITNREIISSNVDDFEYTPKPEF 326
            ....::.|||||:         ..::..|.  ::|.|    |::......:|::|         |
E. coli   149 PLKWVSIDIETTR---------HGELYCIG--LEGCGQRIVYMLGPENGDASSLD---------F 193

  Fly   327 EGNFIVFNEENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMK--------- 382
            |..::.    :..|||::.........|.:|:.:|...||...::..|..|.|.::
E. coli   194 ELEYVA----SRPQLLEKLNAWFANYDPDVIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELE 254

  Fly   383 -QEIGFSKLRDGNYL--SRPAIHMDCLCWVKRDSYLPVGSQGLKAVAKAKL-----------RYD 433
             :|.||   ::|.:.  ::..:.:|.:..:| .::....|..|:.||:..|           |.|
E. coli   255 WREHGF---KNGVFFAQAKGRLIIDGIEALK-SAFWNFSSFSLETVAQELLGEGKSIDNPWDRMD 315

  Fly   434 PVELDPEDMCRMAVEQPQVLANYSVSDA-VATYYLYMKYVHPFIFALNTI--IPMEPDEILRKGS 495
            .::.      |.|.::| .||.|::.|. :.|...:...:.||:....|:  :|::     |.|.
E. coli   316 EIDR------RFAEDKP-ALATYNLKDCELVTQIFHKTEIMPFLLERATVNGLPVD-----RHGG 368

  Fly   496 GTLCETLLMVEAYHAQIVYPNKHQSELNKLSNEGHVLDSETYVGGHVEALESGVFRADIPCRFRL 560
            ..         |....:.:|..|::                   |:|..
E. coli   369 SV---------AAFGHLYFPRMHRA-------------------GYVAP---------------- 389

  Fly   561 DPAMVKQLQEQVDAVLRHAIEVEEGIPLEKVLNLDEVRQE------IVQGLQGLHDIPNRLEQPV 619
                                            ||.||...      ::....||:|        .
E. coli   390 --------------------------------NLGEVPPHASPGGYVMDSRPGLYD--------S 414

  Fly   620 IYHLDVGAMYPNIILTNRLQPSAMVSDLDCAACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRI 684
            :..||..::||:||.|..:.|..:|                         .|...|
E. coli   415 VLVLDYKSLYPSIIRTFLIDPVGLV-------------------------EGMAQP--------- 445

  Fly   685 QQQLETEKFPPLFPGGPQRAFHELSKEDQAAYEKKRLTDYCRKAYKKTKLTKLETRTSTICQKEN 749
            ..:..||.|...:          .|:|..                               |..|
E. coli   446 DPEHSTEGFLDAW----------FSREKH-------------------------------CLPE- 468

  Fly   750 SFYVDTVRAFRDRRYEYKGLTKVAKASVNAAVASGDAAEIKAAKGREVLYDSLQLAHKCILNSFY 814
              .|..:...||.          ||...|                     ..|..|.|.|:|:||
E. coli   469 --IVTNIWHGRDE----------AKRQGN---------------------KPLSQALKIIMNAFY 500

  Fly   815 GYVMRRGARWHSMPMAGIVCLTGSNIITKAREIIERVGRPLEL-DTDGIWCILPGSFPQEFTIHT 878
            |.:.....|:....:|..:.:.|..|:.:.:.:||..|..:.. |||..:..|.|:       |:
E. coli   501 GVLGTTACRFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGA-------HS 558

  Fly   879 SHEKKK--KINISYPNA-VLNTMVKDHFTNDQYHELRKDKENNLPKYDIRDENSIFFEVDGPYLA 940
            ..|..|  :..:.:.|| ...|:.|...|                       :::..|.:..:..
E. coli   559 EEEAAKIGRALVQHVNAWWAETLQKQRLT-----------------------SALELEYETHFCR 600

  Fly   941 MVLPAAKEEGKKLKKRYAVFNFDGTLAEL--KGFEVKRRGELQLIKNFQSSVFEAFLAGSTLEEC 1003
            .::|..:......|||||....:|....:  ||.|..|.....|.:.||..::.........:|
E. coli   601 FLMPTIRGADTGSKKRYAGLIQEGDKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQE- 664

  Fly  1004 YASVAKVADYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEYGAQKSTSISTAKRLAEFLGEQ 1068
              .|.:..|           .|...||...:...|.:.:.|.||.......:..| |||:...::
E. coli   665 --YVRETID-----------KLMAGELDARLVYRKRLRRPLSEYQRNVPPHVRAA-RLADEENQK 715

  Fly  1069 -----MVKDAGLACKYI-ISKKPEGAPVTERAIPLAIFQSEPSVRRHHLRRWLK 1116
                 ..::.| ..||: .:..||         ||. :|..|....|:|.|.|:
E. coli   716 RGRPLQYQNRG-TIKYVWTTNGPE---------PLD-YQRSPLDYEHYLTRQLQ 758

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PolE1	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	45/230 (20%)
		POLBc_epsilon	529..1154	CDD:99918	103/606 (17%)
		DUF1744	1529..1930	CDD:462493
polB	NP_414602.1	PRK05762	1..783	CDD:235595	188/1094 (17%)