DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-epsilon255 and POL1

DIOPT Version :9

Sequence 1:	NP_524462.2	Gene:	DNApol-epsilon255 / 42758	FlyBaseID:	FBgn0264326	Length:	2236	Species:	Drosophila melanogaster
Sequence 2:	NP_014297.3	Gene:	POL1 / 855621	SGDID:	S000005046	Length:	1468	Species:	Saccharomyces cerevisiae

Alignment Length:	1538	Identity:	273/1538 - (17%)
Similarity:	462/1538 - (30%)	Gaps:	559/1538 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 SSKGKVLQNTGKF-VSENRTEGDDF-------------FNEAGYRQSR----------------- 37
            |||.:.|:...|. .:.|.|..||:             .:|..||..:
Yeast     2 SSKSEKLEKLRKLQAARNGTSIDDYEGDESDGDRIYDEIDEKEYRARKRQELLHDDFVVDDDGVG 66

  Fly    38 -------------ENDKIDSKYGFDRVKDSQER-----------TGYLINMHSNEVL----DEDR 74
                         :|...|...|....|||:.:           |..|...||...|    .:.:
Yeast    67 YVDRGVEEDWREVDNSSSDEDTGNLASKDSKRKKNIKREKDHQITDMLRTQHSKSTLLAHAKKSQ 131

  Fly    75 RLIAALDLFFIQMDG--SRFKCTVAYQPYLLI----------RPEDNMHLEVARFLGRKYSGQIS 127
            :....:|.|    |.  ..|:.....:|.:|:          .|.......:.|..|...|...:
Yeast   132 KKSIPIDNF----DDILGEFESGEVEKPNILLPSKLRENLNSSPTSEFKSSIKRVNGNDESSHDA 192

  Fly   128 GLEHITKEDLD------LPNHLSGLQQQYIKLSFLN--QTAMTKVRRELMSAVKRNQERQKSNTY 184
            |:....|.|.|      |....|.|:.|..||.:.|  |..:..|....:.|.||....|.:
Yeast   193 GISKKVKIDPDSSTDKYLEIESSPLKLQSRKLRYANDVQDLLDDVENSPVVATKRQNVLQDT--- 254

  Fly   185 YMQMLAT-SLAQSSAGSEDATLGKRQQDYMDCIVDIREHDVPYHVRVSIDLRIFCGQWYNIRCRS 248
               :||. ..|||.|..||      .:|        .:.|:                   |..|.
Yeast   255 ---LLANPPSAQSLADEED------DED--------SDEDI-------------------ILKRR 283

  Fly   249 GVELPTITCRPDILDRPEPVVLAFDIETTKLPLKFPDAQTDQVMMISYMIDGQGYLITNREIISS 313
            .:...|.|.|.:|..|..|....| :.....|:........:.:..:..:......:...:::..
Yeast   284 TMRSVTTTRRVNIDSRSNPSTSPF-VTAPGTPIGIKGLTPSKSLQSNTDVATLAVNVKKEDVVDP 347

  Fly   314 NVDDFEYTPKPEFEGNFIVFNEENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYD 378
            ..|.|                        |.|:....||...:|:
Yeast   348 ETDTF------------------------QMFWLDYCEVNNTLIL-------------------- 368

  Fly   379 LDMKQEIGFSKLRDGNYLSRPAIHMDCLC----WVKRDSYLPVG-SQGLKAVAKAKLRYDPVELD 438
                  .|..||:|.|.:| ..:.::.||    ::.|:...|.. .:.:..:...|...|.:...
Yeast   369 ------FGKVKLKDDNCVS-AMVQINGLCRELFFLPREGKTPTDIHEEIIPLLMDKYGLDNIRAK 426

  Fly   439 PEDMCRMAVEQPQVLANYSVSDAVATYYLYMKYVHPFIFALNTIIPMEPDEILRKGSGTLCETLL 503
            |:.| :.:.|.|.:   .|.||       |:|.:.|:             :..:....|:...|.
Yeast   427 PQKM-KYSFELPDI---PSESD-------YLKVLLPY-------------QTPKSSRDTIPSDLS 467

  Fly   504 MVEAYHAQIVYPNKHQSELNKLSNEGHVLDSETYVGGHVEALESGVFRADI--PCRFRLDPAMVK 566
            ....||.                           .||:....||.|.:..|  ||...:..|...
Yeast   468 SDTFYHV---------------------------FGGNSNIFESFVIQNRIMGPCWLDIKGADFN 505

  Fly   567 QLQEQVDAVLRHAIEVEEGI----------------PLEKVLNLDEVRQEIV-------QGLQGL 608
            .::......:..:::..:.|                .::.::|..|.:||||       :.:...
Yeast   506 SIRNASHCAVEVSVDKPQNITPTTTKTMPNLRCLSLSIQTLMNPKENKQEIVSITLSAYRNISLD 570

  Fly   609 HDIPNRLE-----------QPVIYHLDVGAMY-----------------------------PNII 633
            ..||..::           |...:.|.:.|:.                             |::|
Yeast   571 SPIPENIKPDDLCTLVRPPQSTSFPLGLAALAKQKLPGRVRLFNNEKAMLSCFCAMLKVEDPDVI 635

  Fly   634 LTNRLQ----------------PS-------------------------AMVSDLDCAA---CDF 654
            :.:|||                |:                         ..:||: |:.   ||.
Yeast   636 IGHRLQNVYLDVLAHRMHDLNIPTFSSIGRRLRRTWPEKFGRGNSNMNHFFISDI-CSGRLICDI 699

  Fly   655 -NKPG-----------------VRCKRSMDWLWRGEMLPASRNEFQ------------------- 682
             |:.|                 |.|::....|......|..:|:..
Yeast   700 ANEMGQSLTPKCQSWDLSEMYQVTCEKEHKPLDIDYQNPQYQNDVNSMTMALQENITNCMISAEV 764

  Fly   683 --RIQQQLETEKFPPLFPG------GPQRA-------FHELSKE---------DQAAYEKKRLTD 723
              |||....|::...|...      |..||       .||.|:.         :::..:|:|..:
Yeast   765 SYRIQLLTLTKQLTNLAGNAWAQTLGGTRAGRNEYILLHEFSRNGFIVPDKEGNRSRAQKQRQNE 829

  Fly   724 YCRKAYKKTKLTKLE-----------TRTSTICQKENSFYVDTVRAFR------DRRYE-YKGLT 770
            ....|...:|..|.:           .:...:....||.|...::.|.      ||..| ...|.
Yeast   830 ENADAPVNSKKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDIDELP 894

  Fly   771 KVAKASVNAAVASGDAA-------EIKAAKGRE------VLYDSLQLAHKCILNSFYGYVMRRGA 822
            .|..:.|:..|.....|       |:|.....|      |..|..|.|.|...||.||.:....:
Yeast   895 SVPPSEVDQGVLPRLLANLVDRRREVKKVMKTETDPHKRVQCDIRQQALKLTANSMYGCLGYVNS 959

  Fly   823 RWHSMPMAGIVCLTGSNIITKAREIIERVGRPLEL-DTDGIWCILPGSFPQEFTIHTSHEKKKKI 886
            |:::.|:|.:|...|..|:...|::.|.:...:.. |||.:            .|.|..:
Yeast   960 RFYAKPLAMLVTNKGREILMNTRQLAESMNLLVVYGDTDSV------------MIDTGCD----- 1007

  Fly   887 NISYPNAVLNTMVKDHFTNDQYHELRKDKENNLPKYDIRDENSIFFEVDGPYLAMVLPAAKEEGK 951
              :|.:|:...:......|::|..|..|                   :|..:..::|.|
Yeast  1008 --NYADAIKIGLGFKRLVNERYRLLEID-------------------IDNVFKKLLLHA------ 1045

  Fly   952 KLKKRYAVF--NFD-----GTLAELKGFEVKRRGELQLIKNFQSSVFEAFLAGSTLEECYASVAK 1009
              ||:||..  |.|     .|:.|:||.::|||....|.::....|....|:....||   ::.:
Yeast  1046 --KKKYAALTVNLDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIHVLNTILSDKDPEE---ALQE 1105

  Fly  1010 VADYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEY-GAQKSTSISTAKRLAEFLGEQMVKDA 1073
            |.||..|:.....:|....:.:::   |..:||..:.| |.:...::..|.|:.:  ..::|| |
Yeast  1106 VYDYLEDIRIKVETNNIRIDKYKI---NMKLSKDPKAYPGGKNMPAVQVALRMRK--AGRVVK-A 1164

  Fly  1074 GLACKYIISKKPEGAPVTERAIPLAIFQSEPSVRRHHLRR-WLKDNTMGDADIRDVLDWNYYIER 1137
            |....::|:|:.|   :...|...|:..:|   |.|.|.. .:|.|.:       :.|..||:|
Yeast  1165 GSVITFVITKQDE---IDNAADTPALSVAE---RAHALNEVMIKSNNL-------IPDPQYYLE- 1215

  Fly  1138 LGGTIQKIITIPAALQGLANPVPRVQHPDWLHKKMLEKNDVLKQRRINEMFTSRPKP-------- 1194
                  |.|..|.                   :::||:.|.....|::|......|.
Yeast  1216 ------KQIFAPV-------------------ERLLERIDSFNVVRLSEALGLDSKKYFRREGGN 1255

  Fly  1195 ---------KPLATEEDKLADMEDLAGKDGGEGAAGCPIVTKR 1228
                     :||   |..:.|:|..  ||.......||...||
Yeast  1256 NNGEDINNLQPL---ETTITDVERF--KDTVTLELSCPSCDKR 1293

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-epsilon255	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	29/207 (14%)
		POLBc_epsilon	529..1154	CDD:99918	153/835 (18%)
		DUF1744	1529..1930	CDD:369900
POL1	NP_014297.3	pol2	29..1227	CDD:273159	247/1438 (17%)
POL1	NP_014297.3	zf-DNA_Pol	1269..1463	CDD:401082	8/27 (30%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900