DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-epsilon255 and Pold1

DIOPT Version :9

Sequence 1:	NP_524462.2	Gene:	DNApol-epsilon255 / 42758	FlyBaseID:	FBgn0264326	Length:	2236	Species:	Drosophila melanogaster
Sequence 2:	NP_067694.1	Gene:	Pold1 / 59294	RGDID:	621839	Length:	1103	Species:	Rattus norvegicus

Alignment Length:	1386	Identity:	264/1386 - (19%)
Similarity:	459/1386 - (33%)	Gaps:	448/1386 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    32 GYRQSRENDKIDSKYGFDRVKDSQERTGYLINMHSNEVLDEDRRLIAALDLFFIQMD---GSRFK 93
            |.|:...:..:..|.....:.|..|.:.:..|:...|.::.:.||..|.:...:..:   |.:|.
  Rat     3 GKRRQAPSSGVPPKRACKGLWDEDEPSQFEENLALLEEIEAENRLQEAEEELQLPPEGIVGGQFS 67

  Fly    94 CTVAYQPY------LLIRPEDN----MHLEVARFLG-----------RKYSGQISGLEHITKEDL 137
             |....|.      |.:.|...    ..||:..::|           .:.|..|.....:|.|..
  Rat    68 -TADIDPRWLRPTPLALDPSTEPLIFQQLEIDHYVGTSPPLPEGPPASRNSVPILRAFGVTDEGF 131

  Fly   138 DLPNHLSGLQQQYIKLS--FLNQTAMTKVRRELMSAVKRNQ-----------------ERQKSNT 183
            .:..|:.|....:...:  ......:::::|||.:|:.|:|                 .|:....
  Rat   132 SVCCHIHGFAPYFYTPAPPGFGAEHLSELQRELNAAISRDQRGGKELSGPAVLAIELCSRESMFG 196

  Fly   184 YY--------------MQMLATSLAQSSAGSEDATLGKRQ-QDYMDCIVDIREHDVPYHVRVSID 233
            |:              .:::|.:......|.....||... ..|        |.:|.:.:|..:|
  Rat   197 YHGHGPSPFLRITLALPRLMAPARRLLEQGIRVPGLGTPSFAPY--------EANVDFEIRFMVD 253

  Fly   234 LRIFCGQWYNIRCRSGV---ELPTITCR-------PDILDRP--------EPV-VLAFDIETTKL 279
            ..|....|..:.....|   |.....|:       .|::..|        .|: ||:||||....
  Rat   254 ADIVGCNWLELPAGKYVRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGR 318

  Fly   280 PLKFPDAQTDQVMMISYM----IDGQGYL---ITNR---EIISSNVDDFEYTPKPEFEGNFIVFN 334
            ...||:.:.|.|:.|..:    .:.:.:|   :|.|   .|:.:.|..:
  Rat   319 KGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSY---------------- 367

  Fly   335 EENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIG-----FSKLRDGN 394
             |.|..|||.:...|:.:.|.:|..||...||.|::.:||....:|....:|     .|.:||.:
  Rat   368 -EREEDLLQAWATFILAMDPDVITGYNIQNFDLPYLISRAQTLKVDRFPFLGRVTGLRSNIRDSS 431

  Fly   395 YLSRPAIHMDCLCWVKRDSYLPVGSQGLKAVAKAKLRYDPVELDPEDMCRMAVEQPQVLANYSVS 459
            :.||..        .:|||.:      :..|.:.::          ||.::.:.:.: |.:|::
  Rat   432 FQSRQV--------GRRDSKV------VSMVGRVQM----------DMLQVLLREYK-LRSYTL- 470

  Fly   460 DAVATYYLYMK---YVHPFIFALNTIIPMEPDEILRKGSGTLCETLLMVEAYHAQIVYPNKHQSE 521
            :||:.::|..:   ..|..|..|     ...:|..|:.....|    :.:|:     .|.:....
  Rat   471 NAVSFHFLGEQKEDVQHSIITDL-----QNGNEQTRRRLAVYC----LKDAF-----LPLRLLER 521

  Fly   522 LNKLSNEGHVLDSETYVGGHVE-ALESGVFRADIPCRFRLDPA----MVKQLQEQV--DAVLRHA 579
            |..|.|             :|| |..:||     |..:.|...    :|.||..|.  :.:|...
  Rat   522 LMVLVN-------------NVEMARVTGV-----PLGYLLSRGQQVKVVSQLLRQAMREGLLMPV 568

  Fly   580 IEVEEGIPLEKVLNLDEVRQEIVQGLQGLHDIPNRLEQPVIYHLDVGAMYPNIILTNRLQPSAMV 644
            ::.|.|        .|.....:::.|:|.:|:|       |..||..::||:|::.:.|..:.::
  Rat   569 VKTEGG--------EDYTGATVIEPLKGYYDVP-------IATLDFSSLYPSIMMAHNLCYTTLL 618

  Fly   645 SDLDCAACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRIQQQLETEKFPPLFPGGPQRAFHELS 709
                       :||.           .:.|....:||      ::|       |.|.:..
  Rat   619 -----------RPGA-----------AQKLGLKPDEF------IKT-------PTGDEFV----- 643

  Fly   710 KEDQAAYEKKRLTDYCRKAYKKTKLTKLETRTSTICQKENSFYVDTVRAFRDRRYEYKGLTKVAK 774
               :|:..|..|...........|..|.|....|          |.:|
  Rat   644 ---KASVRKGLLPQILENLLSARKRAKAELAQET----------DPLR----------------- 678

  Fly   775 ASVNAAVASGDAAEIKAAKGREVLYDSLQLAHKCILNSFYGYVMRRGARWHSMPMAGIVCLTGSN 839
                                |:|| |..|||.|...||.||:.   ||:...:|     ||..|.
  Rat   679 --------------------RQVL-DGRQLALKVSPNSVYGFT---GAQVGKLP-----CLEISQ 714

  Fly   840 IITK-AREIIERVGRPLEL-----------------DTDGIWCILPGSFPQEFTIHTSHEKKKKI 886
            .:|. .|::||:..:.:|.                 |||.:.|        .|.:.:..|.
  Rat   715 SVTGFGRQMIEKTKQLVETKYTLENGYDANAKVVYGDTDSVMC--------RFGVSSVAEA---- 767

  Fly   887 NISYPNAVLNTMVKDHFTNDQYHELRKDKENNLPKYDIRDENSIFFEVDGPYLAMVLPAAKEEGK 951
             :|......| .|..||.:....|..|                ::|    |||.:
  Rat   768 -MSLGREAAN-WVSSHFPSPIRLEFEK----------------VYF----PYLLI---------- 800

  Fly   952 KLKKRYAVFNFDG-----TLAELKGFEVKRRGELQLIKNFQSSVFEAFLAGSTLEECYASVAKVA 1011
             .|||||...|..     ...:.||.|..||....|:.|..:|.....|.....:   .:||...
  Rat   801 -SKKRYAGLLFSSRSDAHDRMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPD---GAVAHAK 861

  Fly  1012 DYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEYGAQKSTSISTAKRLAEFLGEQMVKDAGLA 1076
            |...|:|.:|      .::.:|:. .|.:::...:| |.|...:..|:|:.:       :|.|.|
  Rat   862 DVISDLLCNR------IDISQLVI-TKELTRAAADY-AGKQAHVELAERMRK-------RDPGSA 911

  Fly  1077 ------CKYIISKKPEG-APVTERAIPLAIFQ-SEPSVRRHHLRRWLK-------DNTMGDADIR 1126
                  ..|:|....:| |...:...||.:.: |.|...:::|.:.|.       :..:|:....
  Rat   912 PNLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAE 976

  Fly  1127 DVLDWNYYIERLGGTIQKIITIPAALQG--LANPVPR---------VQHPDWLHKKMLEKNDVLK 1180
            .||        |.|...:..|:..:..|  ||....|         :.|...:.|....:...|.
  Rat   977 SVL--------LRGDHTRCKTVLTSKVGGLLAFTKRRNSCIGCRSVIDHQGAVCKFCQPRESELY 1033

  Fly  1181 QRRINEMFTSRPKPKPLATE---------EDKLADMEDLAGKDGGEGAAGCPIVTKRKRIQLEEH 1236
            |:.::.:.....:...|.|:         ||.:....|            |||...||:::.:..
  Rat  1034 QKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRD------------CPIFYMRKKVRKDLE 1086

  Fly  1237 DDEEAQPQATTWRQALGAPPP 1257
            |.|..       .|..|.|.|
  Rat  1087 DQERL-------LQRFGPPGP 1100

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-epsilon255	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	50/218 (23%)
		POLBc_epsilon	529..1154	CDD:99918	130/669 (19%)
		DUF1744	1529..1930	CDD:369900
Pold1	NP_067694.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..27		4/23 (17%)
		Nuclear localization signal. /evidence=ECO:0000255	4..17		1/12 (8%)
		PTZ00166	80..1096	CDD:240301	247/1302 (19%)
		DNA_polB_delta_exo	300..529	CDD:99820	61/298 (20%)
		POLBc_delta	575..970	CDD:99916	110/563 (20%)
		zf-C4pol	1008..1078	CDD:290959	12/81 (15%)
		CysB motif	1054..1072		3/29 (10%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900