DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-epsilon255 and pole

DIOPT Version :9

Sequence 1:	NP_524462.2	Gene:	DNApol-epsilon255 / 42758	FlyBaseID:	FBgn0264326	Length:	2236	Species:	Drosophila melanogaster
Sequence 2:	NP_001121995.1	Gene:	pole / 553405	ZFINID:	ZDB-GENE-070705-557	Length:	2284	Species:	Danio rerio

Alignment Length:	2306	Identity:	1266/2306 - (54%)
Similarity:	1652/2306 - (71%)	Gaps:	117/2306 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 VLQNTGKFVSENRTEGDDFFNEAG-------YRQSRENDKIDSKYGFDRVKDSQERTGYLINMHS 66
            ||||:|::.::...:.|:..::|.       ..:|:..|.:|.::||:|:|:..|:||:|||||.
Zfish     2 VLQNSGRYKADRGGDQDNQQDDASALSAVKRLERSQFTDSMDERFGFERMKEPGEKTGWLINMHP 66

  Fly    67 NEVLDEDRRLIAALDLFFIQMDGSRFKCTVAYQPYLLIRPEDNMHLEVARFLGRKYSGQISGLEH 131
            .|:||:|:|:|:|:|.:|||.||:|||..:.|:||..|..:.|...||..||.:|:.|:::.||.
Zfish    67 TEILDDDKRMISAVDYYFIQEDGNRFKVALPYKPYFYIATKKNTEREVISFLSKKFQGKVAKLES 131

  Fly   132 ITKEDLDLPNHLSGLQQQYIKLSFLNQTAMTKVRRELMSAVKRNQERQKSNTYYMQMLATSLAQS 196
            :.||||||||||.||::.||||||.....:.||:||:..||::|:||:|||..|..||:::|...
Zfish   132 VPKEDLDLPNHLVGLKRNYIKLSFNTVDDLIKVKREISPAVRKNREREKSNDAYTSMLSSALVGG 196

  Fly   197 SAGSED-ATLGKRQQDYMDCIVDIREHDVPYHVRVSIDLRIFCGQWYNIRCRSGVELPTITCRPD 260
            |..:|| ....|:..:.:|.|:|:||:||||||||||||:|....|||:|.|.....|.|..|.|
Zfish   197 SVVTEDEGGSSKKMTEQLDNILDMREYDVPYHVRVSIDLKIHVAHWYNVRYRGSAYPPEIVRRDD 261

  Fly   261 ILDRPEPVVLAFDIETTKLPLKFPDAQTDQVMMISYMIDGQGYLITNREIISSNVDDFEYTPKPE 325
            :::||:||||||||||||||||||||:|||:|||||||||||:|||||||:|.:::|||:|||||
Zfish   262 LVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGFLITNREIVSEDIEDFEFTPKPE 326

  Fly   326 FEGNFIVFNEENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIGFSKL 390
            :||.|.:|||.:|..|:||:|:|:.|.:|:|.||||||||||||||.|||...|.|.:||||.|.
Zfish   327 YEGPFTIFNEPDEASLIQRWFEHVHETKPNIFVTYNGDFFDWPFVEARAAQLGLSMHREIGFQKD 391

  Fly   391 RDGNYLSRPAIHMDCLCWVKRDSYLPVGSQGLKAVAKAKLRYDPVELDPEDMCRMAVEQPQVLAN 455
            ..|.|.:..|||||||.||||||||||||..|||.|||||.|||||||||:|||||.|:||.||.
Zfish   392 NQGEYKASQAIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEEMCRMATEEPQTLAT 456

  Fly   456 YSVSDAVATYYLYMKYVHPFIFALNTIIPMEPDEILRKGSGTLCETLLMVEAYHAQIVYPNKHQS 520
            ||||||||||||||||||||||||.|||||||||:||||||||||.||||:|||..||:|||.:.
Zfish   457 YSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAYHVNIVFPNKQEQ 521

  Fly   521 ELNKLSNEGHVLDSETYVGGHVEALESGVFRADIPCRFRLDPAMVKQLQEQVDAVLRHAIEVEEG 585
            ..|||:::|||||||||||||||||||||||:||||||:::||....|.::|:..||||||.||.
Zfish   522 VFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFKMNPAAFDFLIQRVERTLRHAIEEEEK 586

  Fly   586 IPLEKVLNLDEVRQEIVQGLQGLHDIPNRLEQPVIYHLDVGAMYPNIILTNRLQPSAMVSDLDCA 650
            ||||:|.|.:||..||.:.|..|.::|||:|.|:|||||||||||||||||||||||||.:..||
Zfish   587 IPLEQVTNFNEVCDEIKRKLISLKEVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCA 651

  Fly   651 ACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRIQQQLETEKFPPLFPGGPQRAFHELSKEDQAA 715
            ||||||||..|:|.|.|.||||::||||:||.|||||||:|||||:||.||.||||||::|:||.
Zfish   652 ACDFNKPGANCQRRMTWQWRGEIMPASRSEFHRIQQQLESEKFPPVFPNGPPRAFHELNREEQAR 716

  Fly   716 YEKKRLTDYCRKAYKKTKLTKLETRTSTICQKENSFYVDTVRAFRDRRYEYKGLTKVAKASVNAA 780
            :|||||.|||||||||..||:||.|.:||||:||||||||||||||||||:|||.||.|..::.|
Zfish   717 HEKKRLGDYCRKAYKKVHLTRLEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSTA 781

  Fly   781 VASGDAAEIKAAKGREVLYDSLQLAHKCILNSFYGYVMRRGARWHSMPMAGIVCLTGSNIITKAR 845
            ..:|||||:|..|..|:||:|||||||||||||||||||:||||:||.||||||.||:||||:||
Zfish   782 QDNGDAAEVKRCKNMEILYESLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCYTGANIITQAR 846

  Fly   846 EIIERVGRPLELDTDGIWCILPGSFPQEFTIHTSHEKKKKINISYPNAVLNTMVKDHFTNDQYHE 910
            |:||::|||||||||||||:||.:||:.|.|.:::|||.|:.||||.|:||.|||:.|||.||.|
Zfish   847 ELIEQIGRPLELDTDGIWCVLPNTFPENFVIKSTNEKKPKVTISYPGAMLNIMVKEGFTNHQYQE 911

  Fly   911 LRKDKENNLPKYDIRDENSIFFEVDGPYLAMVLPAAKEEGKKLKKRYAVFNFDGTLAELKGFEVK 975
            |   .:.....|:.|.|||||||||||||||:|||:|||||||||||||||.||:||||||||||
Zfish   912 L---VDAASLTYETRAENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVK 973

  Fly   976 RRGELQLIKNFQSSVFEAFLAGSTLEECYASVAKVADYWLDVLYSRGSNLPDSELFELISENKSM 1040
            |||||||||.||||||||||.|:||||.|||||||||||||||||:.:|:||:|||||||||:||
Zfish   974 RRGELQLIKIFQSSVFEAFLKGTTLEEVYASVAKVADYWLDVLYSKAANMPDAELFELISENRSM 1038

  Fly  1041 SKKLEEYGAQKSTSISTAKRLAEFLGEQMVKDAGLACKYIISKKPEGAPVTERAIPLAIFQSEPS 1105
            |:|||:||.|||||||||||||||||:||||||||:|:|:||:||||:|||||||||||||:|.|
Zfish  1039 SRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYVISRKPEGSPVTERAIPLAIFQAEES 1103

  Fly  1106 VRRHHLRRWLKDNTMGDADIRDVLDWNYYIERLGGTIQKIITIPAALQGLANPVPRVQHPDWLHK 1170
            |::|.||:|||..::.|.|||.:|||:|||||||..||||||||||||.:.||||||:|||||||
Zfish  1104 VKKHFLRKWLKMPSLHDLDIRSILDWSYYIERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHK 1168

  Fly  1171 KMLEKNDVLKQRRINEMFTSRPK----PKPLATEEDKLADMEDLAGKDGGEGAAGCPIVTKRKRI 1231
            |:|||||:.||::|:|:|||..|    .:.||..|  ..||||||........| ..|.||||||
Zfish  1169 KLLEKNDIYKQKKISELFTSEGKRQVAQQTLAAGE--TPDMEDLAAPQRPVQPA-ILISTKRKRI 1230

  Fly  1232 QLEEHDDEEAQPQATT--WRQALGAPPPIGETRKTIVEWVRFQKKKWKWQQDQRQRNRQASKRTR 1294
            ...|....|.|.|..|  ||:.||.||.:|.||:.|:.|:|:.||||:.|..||:..|:..:...
Zfish  1231 SQGEDSQTETQEQELTQSWREILGPPPAMGATREEILVWLRYHKKKWELQLRQRKERRKRRRLID 1295

  Fly  1295 GEDPP----VVRATGSTATLGGFLRRAQRTLLDQPWQIVQLVPVDDLGHFTVWALIGEELHKIKL 1355
            ||..|    |:|. |.|..||.||||..|::||.||||||:......|.:.:||:||.:||.:||
Zfish  1296 GESQPTGGGVIRG-GPTTGLGSFLRRTARSILDMPWQIVQIAETSHPGLYKLWAVIGSDLHCMKL 1359

  Fly  1356 TVPRIFYVNQRSAAPPEEGQLWRKVNRVLPRSRPVFNLYRYSVPEQLFRDNSLGMLADLATPDIE 1420
            .:||:|||||| ....||....:||||:||||.....||:|:|||.:::::...:.|||:.||||
Zfish  1360 NIPRVFYVNQR-VPKQEEAATCKKVNRILPRSGVACFLYQYTVPEDMYQEHINEINADLSAPDIE 1423

  Fly  1421 GIYETQMTLEFRALMDMGCICGVQREEARRLAQLATKDLETFSIEQLEQRPQTQVKYLASANNRL 1485
            |:||||:.|.||||:.:||:|.|.:...|   .||.::.:||.:|.||.|...|..||...:  :
Zfish  1424 GVYETQVPLLFRALVQLGCMCMVNKHVVR---DLAGREADTFDLEHLEMRSLAQFSYLEPGS--V 1483

  Fly  1486 RKIYLYQHNTPTAKKEIWSLILMPSKKAFVFALDTVRANQMPNMRQLYTAERLALLKNLTAEEQD 1550
            |.||||.|:  ...|.::.|.:...:||.:|.|||||:|||||:..||.|||.|||:..|   ::
Zfish  1484 RHIYLYHHS--QGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLNSLYGAERTALLEKTT---EE 1543

  Fly  1551 KIPVEDYTFEVLIEVDVKQIYRHIQRALTTYKQEHQGPTILCLQTALSARKLSLAMPILLEFPQA 1615
            .:|.|.:.|||..|.|:|.|||.:||.|..||:|.:|||::.:|:....|:|:..|.:|.|||..
Zfish  1544 LLPPEKHLFEVRAENDIKAIYRALQRILLNYKEERRGPTLIAVQSNWELRRLAAGMTVLEEFPVV 1608

  Fly  1616 EIHISDDASLLSGLDWQRQGSRAVIRHFLNLNNVLDLMLDQCRYFHVPIGNMPPDTVLFGADLFF 1680
            .:|:.|:.| .:.|||||.|:|.:|:|:|||::.|....|..||:|:|:||:|.|..:||:|||.
Zfish  1609 PVHVIDEIS-YNVLDWQRHGARRMIKHYLNLDSCLSQAFDMARYYHLPVGNLPQDISIFGSDLFL 1672

  Fly  1681 ARLLQRHNFVLWWSASTRPDLGGREADDSRLLAEFEESISVVQNKAGFYPDVCVELALDSLAVSA 1745
            ||.|::||.:||.|.:.||||||:|||||||:.|.:|..|:..|..|.|..|||||.:.||||:.
Zfish  1673 ARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDERGSMEINSQGCYSTVCVELDIQSLAVNT 1737

  Fly  1746 LLQSTRIQEME-GASSAITFDVMPQVSLEEMI-GTVPAATLPSYDETALCSAAFRVMRSMVNGWL 1808
            :|||..:.:|| |||..::|||:...|||||: |...|..:.|||||||||..||:::|||.||:
Zfish  1738 ILQSQHVNDMEGGASIGVSFDVIQHASLEEMMSGNQGANAVASYDETALCSNTFRILKSMVVGWV 1802

  Fly  1809 REVSINRNIFSDFQIVHFYRWVRSSNALLYDPALRRSLNNLMRKMFLRIIAEFKRLGATIIYADF 1873
            ||::...|:::|.|::|||||:||.::|||||||.|:|.|:|:|:||:::|||||||:::||.:|
Zfish  1803 REITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLLNMMKKIFLQLVAEFKRLGSSVIYGNF 1867

  Fly  1874 NRIILSSGKKTVSDALGYVDYIVQSLRNKEMFHSIQLSFEQCWNFMLWMDQANFSGIRGKLPKGI 1938
            ||:||.:.|:.:.||:.||:||..|:..||:|||:.::|.:||.|:||||.||..|::||||
Zfish  1868 NRLILCTKKRRIDDAIAYVEYITNSIHTKEIFHSLSITFSRCWEFLLWMDPANCGGVKGKLP--- 1929

  Fly  1939 DETVSSIVSTTMIRDSER------NQDDDEDEEEDSENRDPVESNEAEQDQEDELSLELNWTIGE 1997
                ||::...  .|||:      .:|..:||:|..||::..|..:.|:|.||  .:|.||.|.:
Zfish  1930 ----SSLLYGE--HDSEKKKKDGAEEDGSDDEDEHEENQEEDEEQDREEDVED--LIESNWNIMQ 1986

  Fly  1998 HLPDENECREKFESLLTLFMQSL---------------------AEKKTTEQAIKDISHCA---- 2037
            :||....|::.|..:::.::.::                     ...:.::|...|:|...
Zfish  1987 YLPQTASCQKYFLMIISAYIAAVYHSMRQELRRNAPGATPIKRRGATQASQQPSGDVSALPGLIT 2051

  Fly  2038 --------------FDFILKLHKNYGKGK----PS---------------PGLELIRTLIKALSV 2069
                          |....|:.|.....:    ||               |.||.|:.:.:.||:
Zfish  2052 FSQEYVSSELTQNFFTITQKIQKKVSSTRSVTLPSEMFPVLPGSHLPLNNPALEFIKYVCQVLSL 2116

  Fly  2070 DKTLAEQINELRRNMLRLVGIGEFSDLAEWEDPCDSHIINEVICKACNHCRDLDLCKDKHRAMKD 2134
            |..:..|:|:|:|::||||.:||||:.|::.|||.|:::.||||..||.|||||||||. ...:|
Zfish  2117 DANIVNQVNKLKRDLLRLVDVGEFSEDAQFRDPCKSYVLPEVICHHCNFCRDLDLCKDP-AVSQD 2180

  Fly  2135 G--VPVWLCAQCYVAYDNEEIEMRMLDALQRKMMSYVLQDLRCSRCSEIKRENLAEFCTCAGNFV 2197
            |  :|.|.|:.|...||.|.|||.:::|||:|:|||.|||:.|::|..:|..|:..:|:|||:|.
Zfish  2181 GSVLPQWFCSNCQAQYDTESIEMALVEALQKKLMSYTLQDVECAKCKGVKEANMPLYCSCAGDFN 2245

  Fly  2198 PLISGKDIQTLLGTFNKVAANHKMQLLQQTV 2228
            ...:.|.....:..|..:||::.|..|.:|:
Zfish  2246 LTFTTKSFSEQVEVFRNIAAHYNMSFLLETI 2276

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-epsilon255	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	149/202 (74%)
		POLBc_epsilon	529..1154	CDD:99918	460/624 (74%)
		DUF1744	1529..1930	CDD:369900	200/402 (50%)
pole	NP_001121995.1	PolB	97..1164	CDD:223494	743/1069 (70%)
		DNA_polB_epsilon_exo	267..470	CDD:99822	149/202 (74%)
		POLBc_epsilon	530..1152	CDD:99918	460/624 (74%)
		DUF1744	1527..1924	CDD:285663	200/400 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170594652
Domainoid	1	1.000	431	1.000	Domainoid score	I569
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4539
Inparanoid	1	1.050	2462	1.000	Inparanoid score	I28
OMA	1	1.010	-	-		QHG55019
OrthoDB	1	1.010	-	-		D16244at33208
OrthoFinder	1	1.000	-	-		FOG0004282
OrthoInspector	1	1.000	-	-		oto40951
orthoMCL	1	0.900	-	-		OOG6_101586
Panther	1	1.100	-	-	LDO	PTHR10670
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R346
SonicParanoid	1	1.000	-	-		X3018
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800