Protein Alignment PolE1 and POLA1

DIOPT Version :10

• Sequence 1: NP_524462.2
• Gene: PolE1 / 42758
• FlyBaseID: FBgn0264326
• Length: 2236
• Species: Drosophila melanogaster
• Sequence 2: NP_001427735.1
• Gene: POLA1 / 5422
• HGNCID: 9173
• Length: 1489
• Species: Homo sapiens
• Alignment Length: 1178
• Identity: 228/1178 - (19%)
• Similarity: 386/1178 - (32%)
• Gaps: 411/1178 - (34%)

  Fly   241 WYNIRCRSGVELPTITC-------RPDILD-----RPEP-VVLAFDIETTKLPLKFPDAQTDQVM 292
            |..::....:..|...|       :||:::     .|.| ||:||.::|.:      :|:..|..
Human   504 WLEVKSPQLLNQPVSWCKVEAMALKPDLVNVIKDVSPPPLVVMAFSMKTMQ------NAKNHQNE 562

  Fly   293 MI--------SYMIDGQGYLITNREIISSNVDDFEYTPKPEFEGNFIV------------FNE-- 335
            :|        |:.:|                   :..|||.|:.:|.|            |.|
Human   563 IIAMAALVHHSFALD-------------------KAAPKPPFQSHFCVVSKPKDCIFPYAFKEVI 608

  Fly   336 ----------ENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIGFSKL 390
                      ..|..||..|...:.::.|.|||.:|...|:...:..|..|.......:||  :|
Human   609 EKKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKAPHWSKIG--RL 671

  Fly   391 RDGN---------YLSRPAI--HMDCLCWVKRDSYLPVGSQGLKAVAKAKLRYDPVELDPEDMCR 444
            :..|         :..|.|.  .|.|...:.....:...|..|..:.:..|:.:.|.:..|::..
Human   672 KRSNMPKLGGRSGFGERNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQN 736

  Fly   445 MAVEQPQV--LANYSVSDAVATYYLYMKYVHPFIFALNTIIPME----------PDEILRKGSGT 497
            |..|..|:  |..::..||        |::...:..|| ::|:.          ....|..|...
Human   737 MYSESSQLLYLLEHTWKDA--------KFILQIMCELN-VLPLALQITNIAGNIMSRTLMGGRSE 792

  Fly   498 LCETLLMVEAYHAQIVYPNKH--QSELNKLSNEGHVLDSET--YVGGHVEALESGVFRADIPCRF 558
            ..|.||:...|....:.|:|.  :....||.:|...:|.:|  |..|..:|..:|        ..
Human   793 RNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKAAYAG--------GL 849

  Fly   559 RLDPAMVKQLQEQVDAVLRHAIEVEEGIPLEKVLNLDEVRQEIVQGLQGLHDIPNRLEQPVIYHL 623
            .|||.:                                          |.:|       ..|..|
Human   850 VLDPKV------------------------------------------GFYD-------KFILLL 865

  Fly   624 DVGAMYPNIILTNRLQPSAMVSDLDCAACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRIQQQL 688
            |..::||:||.                  :||......:|             ..:|.|::.:..
Human   866 DFNSLYPSIIQ------------------EFNICFTTVQR-------------VASEAQKVTEDG 899

  Fly   689 ETEKFPPLFPGGPQRAFHELSKEDQAAYEKKRLTDYCRKAYKKTKLTKLETRTSTICQKENSFYV 753
            |.|:.|.|  ..|......|.:|
Human   900 EQEQIPEL--PDPSLEMGILPRE------------------------------------------ 920

  Fly   754 DTVRAFRDRRYEYKGLTKVAKASVNAAVASGDAAEIKAAKGREVLYDSLQLAHKCILNSFYGYVM 818
              :|...:||.:.|.|.|  :..:|..:.              :.||..|.|.|...||.||.:.
Human   921 --IRKLVERRKQVKQLMK--QQDLNPDLI--------------LQYDIRQKALKLTANSMYGCLG 967

  Fly   819 RRGARWHSMPMAGIVCLTGSNIITKAREIIERVGRPLEL---DTDGIWCILPGSFPQEFTIHTSH 880
            ...:|:::.|:|.:|...|..|:...:|:::::.  ||:   |||.|
Human   968 FSYSRFYAKPLAALVTYKGREILMHTKEMVQKMN--LEVIYGDTDSI------------------ 1012

  Fly   881 EKKKKINISYPNAVLNTMVKDHFTN-DQYHELR---KDKENNLPKYDIRDENSIFFEVDGPYLAM 941
                             |:..:.|| ::..:|.   |.:.|.|.|.       :..::||.:.::
Human  1013 -----------------MINTNSTNLEEVFKLGNKVKSEVNKLYKL-------LEIDIDGVFKSL 1053

  Fly   942 VLPAAKEEGKKLKKRYAVF----NFDG---TLAELKGFEVKRRGELQLIKNFQSSVFEAFLAGST 999
            :|        ..||:||..    ..||   |..||||.::.||....|.|:..:.|....|:..:
Human  1054 LL--------LKKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQS 1110

  Fly  1000 LEECYASVAK-VADYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEYGAQKSTSISTAKRLAE 1063
            .:....::.| :.:...:||   ..::|.|: ||:   ||:::|..::|..:||...........
Human  1111 RDTIVENIQKRLIEIGENVL---NGSVPVSQ-FEI---NKALTKDPQDYPDKKSLPHVHVALWIN 1168

  Fly  1064 FLGEQMVKDAGLACKYIISKKPEGAPVTERAIPLAIFQSEPSVRRHHLRRWLKDNTMGDADIRDV 1128
            ..|.:.|| ||....|:|.:.......::||......|.:             ||.        .
Human  1169 SQGGRKVK-AGDTVSYVICQDGSNLTASQRAYAPEQLQKQ-------------DNL--------T 1211

  Fly  1129 LDWNYYI-ERLGGTIQKI--------ITIPAALQGLANPVPRVQHPDWLHKKMLEKNDVL----- 1179
            :|..||: :::...:.:|        ..:.|...||.....||.|   .||.  |:||.|
Human  1212 IDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHH---YHKD--EENDALLGGPA 1271

  Fly  1180 -----KQRRINEMFTSRPKPKPLATEEDKLADMEDLAGKDGGEGAAGCPIVTKRKRIQLEEHDDE 1239
                 ::.|..|.|..   |.|....|:...::.|.:|.|.......|..:            |.
Human  1272 QLTDEEKYRDCERFKC---PCPTCGTENIYDNVFDGSGTDMEPSLYRCSNI------------DC 1321

  Fly  1240 EAQPQATTWRQALGAPPPIGETRKTIVEWVRFQKK---KWKWQQDQRQRNRQAS-----KRTRGE 1296
            :|.|...|          :..:.|.|::..||.||   .|...::...|||...     .||...
Human  1322 KASPLTFT----------VQLSNKLIMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQFSRTGPL 1376

  Fly  1297 DPPVVRAT 1304
            .|..::||
Human  1377 CPACMKAT 1384

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PolE1	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	50/248 (20%)
		POLBc_epsilon	529..1154	CDD:99918	117/650 (18%)
		DUF1744	1529..1930	CDD:462493
POLA1	NP_001427735.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	104..129
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	238..257
		DNA-binding. /evidence=ECO:0000255	656..721		12/66 (18%)
		DNA-binding. /evidence=ECO:0000255	1251..1382		36/160 (23%)
		CysB motif. /evidence=ECO:0000250|UniProtKB:P15436	1354..1380		6/25 (24%)