Protein Alignment PolE1 and pola1

DIOPT Version :10

• Sequence 1: NP_524462.2
• Gene: PolE1 / 42758
• FlyBaseID: FBgn0264326
• Length: 2236
• Species: Drosophila melanogaster
• Sequence 2: NP_001292393.1
• Gene: pola1 / 317740
• ZFINID: ZDB-GENE-030114-9
• Length: 1468
• Species: Danio rerio
• Alignment Length: 1257
• Identity: 241/1257 - (19%)
• Similarity: 411/1257 - (32%)
• Gaps: 471/1257 - (37%)

  Fly    56 ERTGYL------INMHSNE------VLDEDRRLIAALDLFFIQMDGSRFKCTVAYQPYLLIRPE- 107
            |||.||      :|:.:.|      ::|..:......:.|.|.    :||.....:.|....|:
Zfish   395 ERTMYLLPREHKVNLATGEKGVAVGMMDVYKEFNEISEKFKIM----KFKSKPVTKNYAFEIPDI 455

  Fly   108 --DNMHLEVARFLGRKYSGQISGLEHITKEDLDLP----NHLSGLQQQYIKLSFLNQTAMTKVRR 166
              .:.:|||      |||.:|..|..      ||.    :|:.|.....::...||:    |:|.
Zfish   456 PSQSEYLEV------KYSAEIPSLPS------DLKGTTFSHVFGTNTSSLEHFLLNR----KIRG 504

  Fly   167 ELMSAVKRNQERQKSNTYYMQMLATSLAQSSAGSEDATLGKRQQDYMDCIVDIREHDVPYHVRVS 231
            .....|   ...|.||.........::||.|             |.:..:.|
Zfish   505 PCWLDV---TTPQLSNQPISWCKVEAVAQKS-------------DLISVVKD------------- 540

  Fly   232 IDLRIFCGQWYNIRCRSGVELPTITCRPDILDRPEPVVLAFDIETTKLPLKFPDAQTDQVMMISY 296
                                          |..|..||::..::|    ::.|....::::.|:.
Zfish   541 ------------------------------LSPPPLVVMSISLKT----VQNPKTHQNEIVSIAA 571

  Fly   297 MIDGQGYLITNREIISSNVDDFEYT-------PKPEFEGNFIVFNEEN----------------- 337
            ::                    .:|       |:|.|:.:|.|.::.|
Zfish   572 LV--------------------HHTFPLDKAPPRPPFQTHFCVVSKPNDCVFPYDFKDAVRKKNG 616

  Fly   338 -------EMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIGFSKLRDGNY 395
                   |..||..|...:.::.|.::|.::...||...:..|..|..:....:||  :||..|
Zfish   617 IVEIAGTERALLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIG--RLRRAN- 678

  Fly   396 LSRPAIHMDCLCWVKRDSYLPVGSQGL--KAVAKAKLRYDPVELDPEDMCRMAVEQPQVLANYSV 458
              .|.:.               |..|.  |:....:|..| :|:..:::.|        ..:|::
Zfish   679 --MPKLG---------------GRSGFAEKSATCGRLVCD-IEISAKELIR--------CKSYNL 717

  Fly   459 SDAVATYYLYMKYVHPFIFALNTIIPMEPDEILRKGSGTLCETLLMVEA--YHAQIVYPNKHQSE 521
            ::.|:.             .|....|:.|.|.:|.........|.::|.  ..|:::.  :...|
Zfish   718 TELVSQ-------------ILKAERPVVPPENIRNFYSDSTHLLYLLELTWMDAKLIL--QIMCE 767

  Fly   522 LN------KLSN-EGHVLDSETYVGGHVEALESGVFRA------DIPCRFRLDPAMVKQLQEQVD 573
            ||      :::| .|:|: |.|.:||..|..|..:..|      .:|     |....|:.|:.:.
Zfish   768 LNVLPLALQITNIAGNVM-SRTLMGGRSERNEYLLLHAFHERDFIVP-----DKQFFKKAQQDLG 826

  Fly   574 AVLRHAIEVEEGIPLEKVLNLDEVRQEIVQGL-----QGLHDIPNRLEQPVIYHLDVGAMYPNII 633
                   |.|||...:...|....:.....||     .|.:|       ..|..||..::||:||
Zfish   827 -------EDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVGFYD-------KFILLLDFNSLYPSII 877

  Fly   634 LTNRLQPSAMVSDLDCAACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRIQQQLETEKFPPLFP 698
            .                  :||    .|..:::   ||......:.|        |.|...|..|
Zfish   878 Q------------------EFN----ICFTTVE---RGATNTRKKTE--------EDEDEIPELP 909

  Fly   699 GGPQRAFHELSKEDQAAYEKKRLTDYCRKAYKKTKLTKLETRTSTICQKENSFYVDTVRAFRDRR 763
                         ||:                      ||   ..|..||       :|...:||
Zfish   910 -------------DQS----------------------LE---MGILPKE-------IRKLVERR 929

  Fly   764 YEYKGLTKVAKASVNAAVASGDAAEIKAAKGREVLYDSLQLAHKCILNSFYGYVMRRGARWHSMP 828
            .:.|.|.|  :..:|..:              .:.||..|.|.|...||.||.:....:|:::.|
Zfish   930 RQVKQLMK--EPDLNPDL--------------YLQYDIRQKALKLTANSMYGCLGFSFSRFYAKP 978

  Fly   829 MAGIVCLTGSNIITKAREIIERVGRPLEL---DTDGIWCILPGSFPQEFTIHTSHEKKKKINISY 890
            :|.:|...|..|:...:|:::|:.  ||:   |||.|
Zfish   979 LAALVTHKGREILMHTKEMVQRMN--LEVIYGDTDSI---------------------------- 1013

  Fly   891 PNAVLNTMVKDHFTN-DQYHELR---KDKENNLPKYDIRDENSIFFEVDGPYLAMVLPAAKEEGK 951
                   |:..:.|| ::..:|.   |.:.|.|.|.       :..::||.:.:::|
Zfish  1014 -------MINTNSTNLEEVFKLGNKVKSEVNKLYKL-------LEIDIDGVFKSLLL-------- 1056

  Fly   952 KLKKRYAVF----NFDG---TLAELKGFEVKRRGELQLIKNFQSSVFEAFLAGSTLEECYASV-- 1007
            ..||:||..    ..||   |..||||.::.||....|.|...:.|....|:....:....::
Zfish  1057 LKKKKYAALMVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIENIQK 1121

  Fly  1008 ------AKVADYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEYGAQKSTSISTAKRLAEFLG 1066
                  .|||:          .|:| ..:||:   :||::|:.::|..:||.............|
Zfish  1122 HLIEIGEKVAN----------GNIP-LNMFEI---HKSLTKEPQDYPDKKSLPHVHVALWINSQG 1172

  Fly  1067 EQMVKDAGLACKYIISKKPEGAPVTERAIPLAIFQSEPSVRRHHLRRWLKDNTMGDADIRDVLDW 1131
            .:.|| ||....|:|.:.......::||..|...|.:|.:.                     ||.
Zfish  1173 GKKVK-AGDTVSYVICQDGSNLAASQRAYTLEQLQKQPGLN---------------------LDT 1215

  Fly  1132 NYYIER-----LGGTIQKIITIPAALQGLANPVPRVQHPDWL-----HKKMLEKNDVLKQRRINE 1186
            .||:.:     :|...:.|..|.|.|  :|.         ||     |.:      ..:|:|.:|
Zfish  1216 QYYLSQQVHPVVGRICEPIEGIDAVL--IAT---------WLGLDPSHFR------AQQQQREDE 1263

  Fly  1187 MFTSRPKPKPLATEEDKLADME 1208
            .......|..| |:|::..|.|
Zfish  1264 ADDFLGAPAQL-TDEERYRDCE 1284

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PolE1	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	37/235 (16%)
		POLBc_epsilon	529..1154	CDD:99918	138/662 (21%)
		DUF1744	1529..1930	CDD:462493
pola1	NP_001292393.1	pol2	58..1232	CDD:273159	224/1185 (19%)
		zf-DNA_Pol	1272..1461	CDD:462651	5/14 (36%)