Protein Alignment DNApol-epsilon255 and Pole

DIOPT Version :9

• Sequence 1: NP_524462.2
• Gene: DNApol-epsilon255 / 42758
• FlyBaseID: FBgn0264326
• Length: 2236
• Species: Drosophila melanogaster
• Sequence 2: NP_001100622.2
• Gene: Pole / 304573
• RGDID: 1594540
• Length: 2283
• Species: Rattus norvegicus
• Alignment Length: 2313
• Identity: 1263/2313 - (54%)
• Similarity: 1650/2313 - (71%)
• Gaps: 124/2313 - (5%)

  Fly     9 VLQNTGKFVSE-------NRTEGDDFFNEAGYR--QSRENDKIDSKYGFDRVKDSQERTGYLINM 64
            ||:|:|:...|       :|.:|......|..|  :|:..||:|.::||:|:|:..||||:||||
  Rat     2 VLRNSGRRHPEPGADSEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINM 66

  Fly    65 HSNEVLDEDRRLIAALDLFFIQMDGSRFKCTVAYQPYLLIRPEDNMHLEVARFLGRKYSGQISGL 129
            |..|:||||:||::|:|.:|||.||||||..:.|:||..|........||:.||.:|:.|:|:.|
  Rat    67 HPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIAARKGCDREVSSFLSKKFQGKIAKL 131

  Fly   130 EHITKEDLDLPNHLSGLQQQYIKLSFLNQTAMTKVRRELMSAVKRNQERQKSNTYYMQMLATSLA 194
            |::.||||||||||.||::.||||||.....:.|||:|:..|||:|:|:..::..|..||::.|.
  Rat   132 ENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQ 196

  Fly   195 QSSAGSEDATLGKRQQDYMDCIVDIREHDVPYHVRVSIDLRIFCGQWYNIRCRSGVELPTITCRP 259
            ..|..:::....|:..|.:|.|||:||:|||||:|:||||||....|||:|.|.......|..|.
  Rat   197 GGSLITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEIARRD 261

  Fly   260 DILDRPEPVVLAFDIETTKLPLKFPDAQTDQVMMISYMIDGQGYLITNREIISSNVDDFEYTPKP 324
            |:::||:||||||||||||||||||||:|||:|||||||||||||||||||:|.:::|||:||||
  Rat   262 DLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKP 326

  Fly   325 EFEGNFIVFNEENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIGFSK 389
            |:||.|.||||.:|:.|:||:|:||.|.:|.|:||||||||||||||.|||::.|.|.|||||.|
  Rat   327 EYEGPFCVFNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQK 391

  Fly   390 LRDGNYLSRPAIHMDCLCWVKRDSYLPVGSQGLKAVAKAKLRYDPVELDPEDMCRMAVEQPQVLA 454
            ...|.|.:...||||||.||||||||||||..|||.|||||.|||||||||||||||.||||.||
  Rat   392 DSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLA 456

  Fly   455 NYSVSDAVATYYLYMKYVHPFIFALNTIIPMEPDEILRKGSGTLCETLLMVEAYHAQIVYPNKHQ 519
            .||||||||||||||||||||||||.|||||||||:||||||||||.||||:|:||.|::|||.:
  Rat   457 TYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQE 521

  Fly   520 SELNKLSNEGHVLDSETYVGGHVEALESGVFRADIPCRFRLDPAMVKQLQEQVDAVLRHAIEVEE 584
            .|.|||:::|||||:|||||||||||||||||:|||||||::||....|.::|:..:|||||.||
  Rat   522 QEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEE 586

  Fly   585 GIPLEKVLNLDEVRQEIVQGLQGLHDIPNRLEQPVIYHLDVGAMYPNIILTNRLQPSAMVSDLDC 649
            .:|:|:..|..||.::|...|..|.|:|||:|.|:|||||||||||||||||||||||:|.:..|
  Rat   587 KVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATC 651

  Fly   650 AACDFNKPGVRCKRSMDWLWRGEMLPASRNEFQRIQQQLETEKFPPLFPGGPQRAFHELSKEDQA 714
            |||||||||..|:|.|.|.||||.:||||:|:.|||.|||:||||||||.||.|||||||:|:||
  Rat   652 AACDFNKPGATCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQA 716

  Fly   715 AYEKKRLTDYCRKAYKKTKLTKLETRTSTICQKENSFYVDTVRAFRDRRYEYKGLTKVAKASVNA 779
            .|||:||.|||||||||..:||:|.|.:||||:||||||||||||||||||:|||.||.|..::|
  Rat   717 KYEKRRLADYCRKAYKKIHMTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSA 781

  Fly   780 AVASGDAAEIKAAKGREVLYDSLQLAHKCILNSFYGYVMRRGARWHSMPMAGIVCLTGSNIITKA 844
            ||..|||:|:|..|..|:||||||||||||||||||||||:||||:||.||||||.||:||||:|
  Rat   782 AVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQA 846

  Fly   845 REIIERVGRPLELDTDGIWCILPGSFPQEFTIHTSHEKKKKINISYPNAVLNTMVKDHFTNDQYH 909
            ||:||::|||||||||||||:||.|||:.|.|.|::.||.|:.||||.|:||.|||:.|||.||.
  Rat   847 RELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKLTISYPGAMLNIMVKEGFTNHQYQ 911

  Fly   910 ELRKDKENNLPKYDIRDENSIFFEVDGPYLAMVLPAAKEEGKKLKKRYAVFNFDGTLAELKGFEV 974
            ||   .|.:...|..|.|||||||||||||||:|||:|||||||||||||||.||:|||||||||
  Rat   912 EL---TEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEV 973

  Fly   975 KRRGELQLIKNFQSSVFEAFLAGSTLEECYASVAKVADYWLDVLYSRGSNLPDSELFELISENKS 1039
            ||||||||||.||||||||||.||||||.|||||||||||||||||:.:|:||||||||||||:|
  Rat   974 KRRGELQLIKIFQSSVFEAFLKGSTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRS 1038

  Fly  1040 MSKKLEEYGAQKSTSISTAKRLAEFLGEQMVKDAGLACKYIISKKPEGAPVTERAIPLAIFQSEP 1104
            ||:|||:||.|||||||||||||||||:||||||||:|:||||:||||:|||||||||||||:||
  Rat  1039 MSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEP 1103

  Fly  1105 SVRRHHLRRWLKDNTMGDADIRDVLDWNYYIERLGGTIQKIITIPAALQGLANPVPRVQHPDWLH 1169
            :||:|.||:|||.:::.|.|||.:|||:|||||||..||||||||||||.:.||||||:||||||
  Rat  1104 TVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLH 1168

  Fly  1170 KKMLEKNDVLKQRRINEMFTSRPKPKPLATE--EDKLA----DMEDLAGKDGGEGAAGCPIVTKR 1228
            ||:|||||:.||::|:|:|....|.:.:..:  |:.|:    ||||...........  |:.|||
  Rat  1169 KKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDFGLTKPYHSTV--PVATKR 1231

  Fly  1229 KRI---QLEEHDDEEAQPQATTWRQALGAPPPIGETRKTIVEWVRFQKKKWKWQQDQRQRNRQAS 1290
            ||:   |.|..|.....|    |::.||.||.:|.|::..:.|::|.||||:.|..||...|:..
  Rat  1232 KRVWESQKESQDITLTVP----WQEVLGQPPSLGTTQEEWLVWLQFHKKKWQLQAQQRLARRKKQ 1292

  Fly  1291 KRTRGEDPPVVRA--TGSTATLGGFLRRAQRTLLDQPWQIVQLVPVDDLGHFTVWALIGEELHKI 1353
            :....||.|.:.|  .|....||.||||..|:::|.||||:|:......|.|.:||:||.:||.|
  Rat  1293 RLESAEDMPRLGAIREGPATGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGSDLHCI 1357

  Fly  1354 KLTVPRIFYVNQRSAAPPEEGQLWRKVNRVLPRSRPVFNLYRYSVPEQLFRDNSLGMLADLATPD 1418
            ||::||:|||||| .|..|:|..:|||||.||||..|:|||.|||||.:::::...:..:|:.||
  Rat  1358 KLSIPRVFYVNQR-VAKAEDGPSYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSAPD 1421

  Fly  1419 IEGIYETQMTLEFRALMDMGCICGVQREEARRLAQLATKDLETFSIEQLEQRPQTQVKYLASANN 1483
            |||:||||:.|.||||:.:||:|.|.::.||   ||:..:.|||::|.||.|...|..||...:
  Rat  1422 IEGVYETQVPLLFRALVQLGCVCVVNKQLAR---QLSGWEAETFALEHLEMRSLAQFSYLEPGS- 1482

  Fly  1484 RLRKIYLYQHNTPTAKKEIWSLILMPSKKAFVFALDTVRANQMPNMRQLYTAERLALLKNLTAEE 1548
             :|.||||.|.  ...|.::.:.:...::|.||.|||||:||||.:..||:||...||
  Rat  1483 -IRHIYLYHHT--QGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSAEHSLLL------- 1537

  Fly  1549 QDKI-----PVEDYTFEVLIEVDVKQIYRHIQRALTTYKQEHQGPTILCLQTALSARKLSLAMPI 1608
             ||:     |...:||||..|.::|.|.|.|||.|..||:|.:|||::.:|::....:|:..:|:
  Rat  1538 -DKVGPNLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAIQSSWELCRLTSEIPV 1601

  Fly  1609 LLEFPQAEIHISDDASLLSGLDWQRQGSRAVIRHFLNLNNVLDLMLDQCRYFHVPIGNMPPDTVL 1673
            |.|||...:.::|..| .:.|||||.|:|.:|||:|||:..|....:..||||:|:||:|.|...
  Rat  1602 LEEFPLVPVRVADKIS-YAVLDWQRHGARRMIRHYLNLDICLSQAFEMSRYFHIPVGNLPEDIST 1665

  Fly  1674 FGADLFFARLLQRHNFVLWWSASTRPDLGGREADDSRLLAEFEESISVVQNKAGFYPDVCVELAL 1738
            ||:||||||.|||||.:||.|.::||||||:||||:||:.||::..:|..|.:|.|..|||||.:
  Rat  1666 FGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINNSGCYSTVCVELDI 1730

  Fly  1739 DSLAVSALLQSTRIQEMEGASS-AITFDVMPQVSLEEMI-GTVPAATLPSYDETALCSAAFRVMR 1801
            .:|||:.:|||..:.:||||.| .|:|||:.|.|||:|: |...|:.|.|||||||||:.||:::
  Rat  1731 QNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALASYDETALCSSTFRILK 1795

  Fly  1802 SMVNGWLREVSINRNIFSDFQIVHFYRWVRSSNALLYDPALRRSLNNLMRKMFLRIIAEFKRLGA 1866
            |||.||::|::...||::|.|::|||||::|..:||:||||.|:|:|:|:|:||::||||||||:
  Rat  1796 SMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGS 1860

  Fly  1867 TIIYADFNRIILSSGKKTVSDALGYVDYIVQSLRNKEMFHSIQLSFEQCWNFMLWMDQANFSGIR 1931
            ::|||:||||||.:.|:.:.|||.||:||..|:.:||:|||:.:||.:||.|:||||.:|:.||:
  Rat  1861 SVIYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIK 1925

  Fly  1932 GKLPKGIDETVSSIVSTTMIRDSERNQDDDEDEEEDSENRDPVESNEAEQDQEDELSLELNWTIG 1996
            ||:|        |.:....:|:.:....::.::|||:| :|..|..:..|.:.::| ||.||.|.
  Rat  1926 GKVP--------SSIHCGQVREQDSQTREETEDEEDNE-KDEEEEEDMGQSEVEDL-LENNWNIL 1980

  Fly  1997 EHLPDENECREKFESLLTLFM----QSL-------------AEKKTTEQ---------------- 2028
            :.||....|:..|..:::.::    ||:             .::|...|
  Rat  1981 QFLPQAASCQSYFLMIVSAYIVAVYQSMKDELRHSAPGSTPVKRKGASQFSQEAEGAAGALPGMI 2045

  Fly  2029 ------AIKDISHCAFDFILKLHK----NYGKGKPS---------------PGLELIRTLIKALS 2068
                  ...:::...|....|:.|    :....:||               |.||.|:.:.|.||
  Rat  2046 TFSQDYVANELTQTFFTITQKIQKKVTGSRNTAEPSEMFPVLPGSHLLLNNPALEFIKYVCKVLS 2110

  Fly  2069 VDKTLAEQINELRRNMLRLVGIGEFSDLAEWEDPCDSHIINEVICKACNHCRDLDLCKDKHRAMK 2133
            :|..:..|:|:|.|::||||.:||||:.|::.|||.|:::.||||.:||.|||||||||...: :
  Rat  2111 LDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDSSFS-Q 2174

  Fly  2134 DG--VPVWLCAQCYVAYDNEEIEMRMLDALQRKMMSYVLQDLRCSRCSEIKRENLAEFCTCAGNF 2196
            ||  :|.|||:.|...||:..||..:::|||||:|::.||||.|.:|..:|..::..:|.|||:|
  Rat  2175 DGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCGCAGDF 2239

  Fly  2197 VPLISGKDIQTLLGTFNKVAANHKMQLLQQTVHQALTT 2234
            |..|..:.....:..|..:|..:.|..||:.:...|.|
  Rat  2240 VLTIHTEVFMEQIRIFQNIAKYYNMSYLQEAIEWLLQT 2277

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-epsilon255	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	154/202 (76%)
		POLBc_epsilon	529..1154	CDD:99918	462/624 (74%)
		DUF1744	1529..1930	CDD:369900	202/407 (50%)
Pole	NP_001100622.2	PolB	99..1165	CDD:223494	745/1068 (70%)
		Lyase_I_like	<125..>231	CDD:294018	49/105 (47%)
		DNA_polB_epsilon_exo	268..471	CDD:99822	154/202 (76%)
		POLBc_epsilon	531..1153	CDD:99918	462/624 (74%)
		DUF1744	1528..1924	CDD:285663	202/404 (50%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166352704
Domainoid	1	1.000	429	1.000	Domainoid score	I576
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4539
Inparanoid	1	1.050	2396	1.000	Inparanoid score	I29
OMA	1	1.010	-	-		QHG55019
OrthoDB	1	1.010	-	-		D16244at33208
OrthoFinder	1	1.000	-	-		FOG0004282
OrthoInspector	1	1.000	-	-		oto98916
orthoMCL	1	0.900	-	-		OOG6_101586
Panther	1	1.100	-	-	LDO	PTHR10670
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3018
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	14	13.810