DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-epsilon255 and Pola1

DIOPT Version :9

Sequence 1:	NP_524462.2	Gene:	DNApol-epsilon255 / 42758	FlyBaseID:	FBgn0264326	Length:	2236	Species:	Drosophila melanogaster
Sequence 2:	NP_032918.1	Gene:	Pola1 / 18968	MGIID:	99660	Length:	1465	Species:	Mus musculus

Alignment Length:	1338	Identity:	245/1338 - (18%)
Similarity:	431/1338 - (32%)	Gaps:	468/1338 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly    28 FNEAGYRQSRENDKIDSK----YGFDRVKDSQER-----------TGYLINMHSNEV---LDEDR 74
            |.:..:.:|.:.:|:|.|    ..:|:..:..||           |.||.|...:..   :|:|.
Mouse   255 FEDGDFDESMDTEKVDEKPVTAKTWDQETEPVERVEHEADPERGTTSYLENFLPDVSCWDIDQDD 319

  Fly    75 RLIAALDLFFIQMDGSRFKCT------VAYQPYLLIRPEDNMHLEVARFL-GRKYSGQISG---- 128
            ..|..    .:|:|.|.....      ..:|.|.|...||..:.....|| |:.:...:..
Mouse   320 ESIPQ----EVQVDSSNLPLVKGADDEQVFQFYWLDAYEDPYNQPGVVFLFGKVWIESVKTHVSC 380

  Fly   129 ---LEHITKEDLDLPNHLSGLQQQYIKLSFLNQTA----MTKVRRELMSAVKRNQERQKSNTYYM 186
               :::|.:....||..:.      ..|:...:||    |..|..|..|.:        |..|.:
Mouse   381 CVMVKNIERTLYFLPREMK------FDLNTGKETAIPVTMKDVYEEFDSKI--------SAKYKI 431

  Fly   187 QMLATSLAQSSAGSEDATLGKRQQDYMDCIVDIREHDVPYHVRVSIDLRIF-------------- 237
            ....:.:.:.:...|...:.:: .:|::.........:|.:::......:|
Mouse   432 MKFKSKIVEKNYAFEIPDVPEK-SEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLELFLMNR 495

  Fly   238 -----CGQWYNIRCRSGVELPTITC-------RPDILD-----RPEP-VVLAFDIETTKLPLKFP 284
                 |  |..::....:..|...|       :||:::     .|.| ||::|.::|.:
Mouse   496 KIKGPC--WLEVKNPQLLNQPISWCKFEVMALKPDLVNVIKDVSPPPLVVMSFSMKTMQ------ 552

  Fly   285 DAQTDQVMMI--------SYMIDGQGYLITNREIISSNVDDFEYTPKPEFEGNFIV--------- 332
            :.|..|..:|        |:.:|                   :..|:|.|:.:|.|
Mouse   553 NVQNHQHEIIAMAALVHHSFALD-------------------KAPPEPPFQTHFCVVSKPKDCIF 598

  Fly   333 ---FNE------------ENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMK 382
               |.|            ..|..|:..|...:.::.|.|:|.:|...|:...:..|.....:...
Mouse   599 PCDFKEVISKKNMKVEIAATERTLIGFFLAKVHKIDPDILVGHNICSFELEVLLQRINECKVPYW 663

  Fly   383 QEIGFSKLRDGN---------YLSRPAI--HMDCLCWVKRDSYLPVGSQGLKAVAKAKLRYDPVE 436
            .:||  :||..|         :..|.|.  .|.|...:.....:...|..|..:.:..|:.:.:.
Mouse   664 SKIG--RLRRSNMPKLGSRSGFGERNATCGRMICDVEISAKELIHCKSYHLSELVQQILKTERIV 726

  Fly   437 LDPEDMCRMAVEQPQVLANYSVSDAVATYYLYM--------KYVHPFIFALNTIIPME------- 486
            :..|::..|          ||.|    :|.||:        :::...:..|| ::|:.
Mouse   727 IPTENIRNM----------YSES----SYLLYLLEHIWKDARFILQIMCELN-VLPLALQITNIA 776

  Fly   487 ---PDEILRKGSGTLCETLLMVEAYHAQIVYPNKH--QSELNKLSNEGHVLDSET--YVGGHVEA 544
               ....|..|.....|.||:...|....:.|:|.  :....||.:|...:|.:|  |..|..:|
Mouse   777 GNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKA 841

  Fly   545 LESGVFRADIPCRFRLDPAMVKQLQEQVDAVLRHAIEVEEGIPLEKVLNLDEVRQEIVQGLQGLH 609
            ..:|        ...|||.:                                          |.:
Mouse   842 TYAG--------GLVLDPKV------------------------------------------GFY 856

  Fly   610 DIPNRLEQPVIYHLDVGAMYPNIILTNRLQPSAMVSDLDCAACDFNKPGVRCKRSMDWLWRGEML 674
            |       ..|..||..::||:||.                  :||......:|
Mouse   857 D-------KFILLLDFNSLYPSIIQ------------------EFNICFTTVQR----------- 885

  Fly   675 PASRNEFQRIQQQLETEKFPPLFPGGPQRAFHELSKEDQAAYEKKRLTDYCRKAYKKTKLTKLET 739
              ..:|.|:..:..|.|:.|.|  ..|......|.:|
Mouse   886 --VTSEVQKATEDEEQEQIPEL--PDPNLEMGILPRE---------------------------- 918

  Fly   740 RTSTICQKENSFYVDTVRAFRDRRYEYKGLTKVAKASVNAAVASGDAAEIKAAKGREVLYDSLQL 804
                            :|...:||.:.|.|.|  :..:|..:.              :.||..|.
Mouse   919 ----------------IRKLVERRKQVKQLMK--QQDLNPDLV--------------LQYDIRQK 951

  Fly   805 AHKCILNSFYGYVMRRGARWHSMPMAGIVCLTGSNIITKAREIIERVGRPLEL---DTDGIWCIL 866
            |.|...||.||.:....:|:::.|:|.:|...|..|:...:::::::.  ||:   |||.|
Mouse   952 ALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQKMN--LEVIYGDTDSI---- 1010

  Fly   867 PGSFPQEFTIHTSHEKKKKINISYPNAVLNTMVKDHFTN-DQYHELR---KDKENNLPKYDIRDE 927
                                           |:..:.|| ::..:|.   |.:.|.|.|.
Mouse  1011 -------------------------------MINTNSTNLEEVFKLGNKVKSEVNKLYKL----- 1039

  Fly   928 NSIFFEVDGPYLAMVLPAAKEEGKKLKKRYAVF----NFDG---TLAELKGFEVKRRGELQLIKN 985
              :..::|..:.:::|        ..||:||..    ..||   |..||||.::.||....|.|:
Mouse  1040 --LEIDIDAVFKSLLL--------LKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKD 1094

  Fly   986 FQSSVFEAFLAGSTLEECYASVAK-VADYWLDVLYSRGSNLPDSELFELISENKSMSKKLEEYGA 1049
            ..:.|....|:..:.:....::.| :.:...:||   ..::|.|: ||:   ||:::|..::|..
Mouse  1095 TGNFVIGQILSDQSRDTIVENIQKRLIEIGENVL---NGSVPVSQ-FEI---NKALTKDPQDYPD 1152

  Fly  1050 QKSTSISTAKRLAEFLGEQMVKDAGLACKYIISKKPEGAPVTERAIPLAIFQSEPSVRRHHLRRW 1114
            :||.............|.:.|| ||....|:|.:.......|:||......|.
Mouse  1153 RKSLPHVHVALWINSQGGRKVK-AGDTVSYVICQDGSNLTATQRAYAPEQLQK------------ 1204

  Fly  1115 LKDNTMGDADIRDVLDWNYY--------IERLGGTIQKIITIPAAL-QGLANPVPRVQHPDWLHK 1170
             .||.        .:|..||        :.|:...|..|..:..|| .||.:...||..   .||
Mouse  1205 -LDNL--------AIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQ---YHK 1257

  Fly  1171 KMLEKNDVLKQRRINEMFTSRPKPKPLATEEDKLADME 1208
            .  |:||.|           ...|..| |:|:|..|.|
Mouse  1258 D--EENDAL-----------LGGPAQL-TDEEKYKDCE 1281

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-epsilon255	NP_524462.2	DNA_polB_epsilon_exo	266..469	CDD:99822	45/246 (18%)
		POLBc_epsilon	529..1154	CDD:99918	119/650 (18%)
		DUF1744	1529..1930	CDD:369900
Pola1	NP_032918.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	20..39
		DNA_pol_alpha_N	41..101	CDD:289058
		pol2	56..1229	CDD:273159	224/1267 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	105..135
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	261..297		7/35 (20%)
		DNA_polB_alpha_exo	539..771	CDD:99819	49/273 (18%)
		DNA-binding. /evidence=ECO:0000255	654..719		12/66 (18%)
		POLBc_alpha	836..1246	CDD:99915	117/640 (18%)
		DNA-binding. /evidence=ECO:0000255	1249..1380		15/50 (30%)
		zf-DNA_Pol	1269..1458	CDD:286132	6/14 (43%)
		CysB motif	1352..1378
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900