DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rassf and MICAL3

DIOPT Version :9

Sequence 1:	NP_651126.3	Gene:	Rassf / 42734	FlyBaseID:	FBgn0039055	Length:	806	Species:	Drosophila melanogaster
Sequence 2:	NP_056056.2	Gene:	MICAL3 / 57553	HGNCID:	24694	Length:	2002	Species:	Homo sapiens

Alignment Length:	1172	Identity:	218/1172 - (18%)
Similarity:	332/1172 - (28%)	Gaps:	456/1172 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 CHKCGKPVYFAERKQSIGYDWHPECLRCEECGKRLNPGQHA-----------EHKSVPYCH---- 53
            |:.|.|.||..||..:.|..:|..|.:||.|...|....:|           .|    ||:
Human   764 CYFCQKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPH----YCYRLSG 824

  Fly    54 -----VPCYGALFGPQLFGH----------------GTRVESHKSYGVKGAQKP----------- 86
                 .|....|.|.:..|.                .:..|.....||.|.::|
Human   825 YAQRKRPAVAPLSGKEAKGPLQDGATTDANGRANAVASSTERTPGSGVNGLEEPSIAKRLRGTPE 889

  Fly    87 -----------------------------------TGAQAN-------------------GPPLP 97
                                               |||:.:                   .|.||
Human   890 RIELENYRLSLRQAEALQEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEGEEEEEEPRLP 954

  Fly    98 RDHL-------------------ESKLKVYNQF---------------------YDNKSLEIRSR 122
            ...|                   |.:|:....|                     ||.:  |..|.
Human   955 PSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGNEEEEEEEEEYEEEEEEDYDEE--EEESS 1017

  Fly   123 EVNNRLV---------LEGALRVYWGVQGVIHLKE-DDDQRILVRKRNSCR--------VSKAAN 169
            |..|:.:         ||....|:|     .|::| ::::|:.....:|..        :.:..:
Human  1018 EAGNQRLQQVMHAADPLEIQADVHW-----THIREREEEERMAPASESSASGAPLDENDLEEDVD 1077

  Fly   170 ESSSDKENEASESLAPPTTTTAEVDQLSTDVSLSESMTFDS-------CSLNEISELPTTPEDAS 227
            ...::.|.||:|. ..|..|.||:|.   |...|:|.: |:       |.....:||.....:..
Human  1078 SEPAEIEGEAAED-GDPGDTGAELDD---DQHWSDSPS-DADRELRLPCPAEGEAELELRVSEDE 1137

  Fly   228 ANTTANSKEQTNGNVCNDDEDTTTTDS--SGTLVEAPTASTSCVSSTLP---------SKLDRL- 280
            ....|:.|.|..|    ..:.|:...|  ...|:..|..|.|.....||         |..:||
Human  1138 EKLPASPKHQERG----PSQATSPIRSPQESALLFIPVHSPSTEGPQLPPVPAATQEKSPEERLF 1198

  Fly   281 -------EKLDWDDIDDLLQVERRHNDKDRIYETMPVKLPSSQ------SSSDSSPSKTST---- 328
                   ||...|...||..|   |:.    ..:.||.||.::      |.....|...||
Human  1199 PEPLLPKEKPKADAPSDLKAV---HSP----IRSQPVTLPEARTPVSPGSPQPQPPVAASTPPPS 1256

  Fly   329 ---------ETTTTTESSSTQS--------ASTNT-------SSTDDFMTATGSLTA-------- 361
                     .:|..|..|.|||        |.|:|       .|..|.....||..|
Human  1257 PLPICSQPQPSTEATVPSPTQSPIRFQPAPAKTSTPLAPLPVQSQSDTKDRLGSPLAVDEALRRS 1321

  Fly   362 ------------NTNTQNTTTVSTTETSLDNFETCDDATLKPIDFEDF--KRSVHQDYVNGANSF 412
                        ...:...|.|..::....:|.|   ...:|:..:.:  ::|...:.::.....
Human  1322 DLVEEFWMKSAEIRRSLGLTPVDRSKGPEPSFPT---PAFRPVSLKSYSVEKSPQDEGLHLLKPL 1383

  Fly   413 TEPN-------EG-TLKRNQPIDPS--------------RIHDSLKLYGENSAM----SKSFNCE 451
            :.|.       || .|....|..||              .....|.|:|.:|.|    |:|||..
Human  1384 SIPKRLGLPKPEGEPLSLPTPRSPSDRELRSAQEERRELSSSSGLGLHGSSSNMKTLGSQSFNTS 1448

  Fly   452 HALRSIDPTLINDTMNLRSS------VGSPHSAQRQYALQKSGSATVT--------SRDQKKPYQ 502
            .:.....|          ||      .|...:..|:...:...:|:|.        .|..::|.|
Human  1449 DSAMLTPP----------SSPPPPPPPGEEPATLRRKLREAEPNASVVPPPLPATWMRPPREPAQ 1503

  Fly   503 QGRQLFEKGINRSK---------------------------SGPSCFVYSDSDDDD--------- 531
            ..|:...|....|.                           :.|||:...:.....
Human  1504 PPREEVRKSFVESVEEIPFADDVEDTYDDKTEDSSLQEKFFTPPSCWPRPEKPRHPPLAKENGRL 1568

  Fly   532 ---EATLRPQ-----------------RMATIRRS-------DIPQRYI--QIQMDC-----YPK 562
               |.||:||                 ||....:|       |...|.:  ..||:.     .|:
Human  1569 PALEGTLQPQKRGLPLVSAEAKELAEERMRAREKSVKSQALRDAMARQLSRMQQMELASGAPRPR 1633

  Fly   563 ENVAAASEGESSRADAPSITSGAAAGDELTQTEDLYTASEGVDGP-DGDGSAGLHVTEDGVVLRR 626
            :..:|.|:|:..|.|:|  |.....|.|....:...|:.|.:..| |..|..|...:.:|
Human  1634 KASSAPSQGKERRPDSP--TRPTLRGSEEPTLKHEATSEEVLSPPSDSGGPDGSFTSSEG----- 1691

  Fly   627 PPRTGASAIKRRSGNRRSRTKLKRRCSINGHYYNRETS-FFTPPYGSQMSVWVSSMVTTTEVINL 690
               :...:.||.|.....|.|.:::....|....:.:| ..........|:|.|
Human  1692 ---SSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSSNLLEEAAAKPKSLWKS----------- 1742

  Fly   691 VLEKYKVD--------SSPGNFSLFIVRDNGEQKRLKDDEYPLITRVTLGPHEDVARIFLVDSRK 747
            |...||.|        |.|...|.....|:|:.:.|......|..|..|...||
Human  1743 VFSGYKKDKKKKADDKSCPSTPSSGATVDSGKHRVLPVVRAELQLRRQLSFSED----------- 1796

  Fly   748 TDEISNEVAQFLNLSLPECRAILERYDQELAREV---------AKIKERYAELRRRIVSRMESLK 803
            :|..|::|              ||:..|:..||.         ||:..|..:..||...:.|..:
Human  1797 SDLSSDDV--------------LEKSSQKSRREPRTYTEEELNAKLTRRVQKAARRQAKQEELKR 1847

  Fly   804 VH 805
            :|
Human  1848 LH 1849

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rassf	NP_651126.3	LIM_TLP_like	4..57	CDD:188785	19/72 (26%)
		UBQ	660..744	CDD:294102	19/92 (21%)
		Nore1-SARAH	762..799	CDD:293125	10/45 (22%)
MICAL3	NP_056056.2	Monooxygenase domain. /evidence=ECO:0000250\|UniProtKB:Q8VDP3	2..494
		FAD_binding_3	87..>274	CDD:279792
		NAD_binding_8	91..>119	CDD:290186
		CH	524..619	CDD:278723
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	658..706
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:24440334	663..684
		LIM_Mical	764..818	CDD:188823	16/57 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	835..883		8/47 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	907..1313		83/428 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1335..1776		86/474 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1791..1821		11/54 (20%)
		DUF3585	1850..1971	CDD:288945	218/1172 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG1700
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810