Protein Alignment klg and robo4

DIOPT Version :9

• Sequence 1: NP_524454.2
• Gene: klg / 42707
• FlyBaseID: FBgn0017590
• Length: 545
• Species: Drosophila melanogaster
• Sequence 2: XP_689255.3
• Gene: robo4 / 560765
• ZFINID: ZDB-GENE-020809-1
• Length: 1134
• Species: Danio rerio
• Alignment Length: 517
• Identity: 109/517 - (21%)
• Similarity: 180/517 - (34%)
• Gaps: 103/517 - (19%)

  Fly    57 LLHAHAGSGGFAVEAAISNRGSNSRSMSNVQQSAVAASTLTATLPRFLSRGHTYRAVVGDTLVLP 121
            |:|..|.....|      :|...||.:|.:            .|||.:.........||....|.
Zfish    45 LMHQRASHRDRA------HRRKGSRLVSEI------------NLPRIVHHPSDVVVRVGSPATLS 91

  Fly   122 CQVENLGNFVLLWRRGTNVLTASNIMVTRDERVRLIDGYNLEISDLEPQDAGD-----YVC---- 177
            |:.|......:.|.|....|.... |..:.:.:.|.|| :|....:.|...|.     |.|
Zfish    92 CRAEGNPEPTIQWLRNGQPLDTDK-MDAQSQPIVLPDG-SLFFFSVVPGRKGQSHEAVYACIAHN 154

  Fly   178 QISDKINRD-QVHTV----EILVPPSVRAIPTSGQLQARKGGPITLECKGS-GNPVPSIYWTKKS 236
            .|.:..:|: .:|..    :..|.||        .::...|...|:.|... |:|.|::.| :|.
Zfish   155 SIGNATSRNASLHIAALREDFRVQPS--------DVEVAIGEMATINCSPPVGHPEPNVTW-RKD 210

  Fly   237 G--ANKSTARIGD--GPILTLEKLERQQAGVYQCTADNGVGDPVTVDMRLDVL-------YPPDI 290
            |  .|.|.....:  |.:: :...::..:|||.|.|.|.:|...:...||.||       .|.|:
Zfish   211 GILINSSNEHYTELKGKLI-IAPAQKNDSGVYSCIASNMIGVRESRAARLSVLAKPVLLRKPEDV 274

  Fly   291 QVEKSWIHSGEGFEAKLVCIVFADPVATVSWYQNSFPIQSTDRRIMATRANRHMLTIRHIQQEDF 355
            .|:.       |..|:..|....||:.::.|.:...|:.:....|...    |.|.|.::..:|.
Zfish   275 SVQL-------GESAQFFCEADGDPMPSIEWSREQGPLPNGRYLINPD----HSLQIHYVTAQDM 328

  Fly   356 GNYSCVADNSLGRS----RKYMELSGRPGAAEFYSPKWGRSPDSYNLT------------WKIDS 404
            |.|||..:|.||.|    :..:|.:|.....:.:...........|:|            ||:.|
Zfish   329 GRYSCTVENKLGVSVASAQLLVEDAGGTRLRDLHKELSALRVSLENVTVMSTASNMSQVMWKLQS 393

  Fly   405 YPP----LEEVRLLYRRVQMNETYQQPGRWHDFILTPEHRPASEPLTHIMSYTIKNLHPGGYYEA 465
            :..    ||...:|||.:     ......|     |.:..|.....||     :..|..|..||.
Zfish   394 FGSQPHYLEGFEVLYRSL-----LPASSDW-----TAQRVPQPSLHTH-----VGPLKRGYKYEF 443

  Fly   466 IVQAKNRYGWNEVSDIFQFVVATNSQDIGPEDAEVVASSKSRSNSASIGSLPGSTVLHTALL 527
            .|:......:...|:.....|........|:|..:...:: |:::..:...|.....|..::
Zfish   444 KVRPYGSSLYGRESNTRHLRVPEIVPSAAPQDVSITMPAE-RNDTVHLSWEPPPHDAHNGII 504

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
klg	NP_524454.2	DUF1370	63..>124	CDD:284518	12/60 (20%)
		IG_like	109..195	CDD:214653	20/99 (20%)
		Ig	118..191	CDD:143165	18/82 (22%)
		IG_like	205..274	CDD:214653	18/73 (25%)
		IGc2	213..273	CDD:197706	18/64 (28%)
		IGc2	301..367	CDD:197706	17/65 (26%)
		FN3	392..486	CDD:238020	21/109 (19%)
robo4	XP_689255.3	Ig1_Robo	70..169	CDD:143317	23/100 (23%)
		I-set	71..168	CDD:254352	21/98 (21%)
		I-set	175..261	CDD:254352	24/95 (25%)
		Ig2_Robo	177..261	CDD:143201	24/93 (26%)
		I-set	265..350	CDD:254352	23/95 (24%)
		Ig	282..350	CDD:299845	19/71 (27%)
		FN3	373..448	CDD:214495	20/89 (22%)
		FN3	472..560	CDD:238020	5/34 (15%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3510
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.900