DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dcr-1 and DCL4

DIOPT Version :9

Sequence 1:	NP_524453.1	Gene:	Dcr-1 / 42693	FlyBaseID:	FBgn0039016	Length:	2249	Species:	Drosophila melanogaster
Sequence 2:	NP_197532.3	Gene:	DCL4 / 832154	AraportID:	AT5G20320	Length:	1702	Species:	Arabidopsis thaliana

Alignment Length:	2282	Identity:	459/2282 - (20%)
Similarity:	726/2282 - (31%)	Gaps:	938/2282 - (41%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 RDFQVELLATAYERNTIICLGHRSSKEFIALKLLQELSRRARRHGR-VSVYLSCEVGTSTEPCSI 81
            |.:||||...|.|.|.|:.||....|..||:.|:.||........: |.::|:..|....:...:
plant   125 RRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKV 189

  Fly    82 ------YTMLTHLTDLRV------WQEQPDMQIPFDHCWTDYHVSILRPEGFLYLLETRELLLSS 134
                  :.:..|....|:      |:.:....          .|.::.|:..|:.|:...:.:..
plant   190 IADSVNFKVAIHCGGKRIVKSHSEWEREIAAN----------EVLVMTPQILLHNLQHCFIKMEC 244

  Fly   135 VELIVLEDCH-----DSAVYQRIRPLFENHIMPAPPADRPRILGL-AGPLHSAGC-ELQQLSAML 192
            :.|::.::||     .:..|..|..:|    ..:....||||.|: |.|:...|. :.:.||..:
plant   245 ISLLIFDECHHAQQQSNHPYAEIMKVF----YKSESLQRPRIFGMTASPVVGKGSFQSENLSKSI 305

  Fly   193 ATLEQSVLCQIETASDIVTVLRYCSRPHEYIVQCAPFEMDELSLVLADVLNTHKSFLLDHRYDPY 257
            .:||..:..::.:....|.:..:.|.|   :|:                :..::|.|.|
plant   306 NSLENLLNAKVYSVESNVQLDGFVSSP---LVK----------------VYYYRSALSD------ 345

  Fly   258 EIYGTDQFMDELKDIPDPKVDPLNVINSLLVVLHEMGPWCTQRAAHHFYQCNEKLKVKTPHERHY 322
            ....|.::.:.|:||....:..|    .||:..|:     ||..      .:.|..:|..|:.
plant   346 ASQSTIRYENMLEDIKQRCLASL----KLLIDTHQ-----TQTL------LSMKRLLKRSHDN-- 393

  Fly   323 LLYCL--------VSTALIQLYSLCEHAFHRHLGSGSDSRQTIERYSSPKVRRLLQTLRCFKPEE 379
            |:|.|        :..|.|||.|  :|.........:...:..:.|.|.....|
plant   394 LIYTLLNLGLWGAIQAAKIQLNS--DHNVQDEPVGKNPKSKICDTYLSMAAEAL----------- 445

  Fly   380 VHTQADGLRRMRHQVDQADFNRLSHTLESKCRMVDQMDQPPTETRALVATLEQILHTTEDRQTNR 444
                :.|:.:..:..|......|               :.|..:|.||..::.:
plant   446 ----SSGVAKDENASDLLSLAAL---------------KEPLFSRKLVQLIKIL----------- 480

  Fly   445 SAARVTPTPTPAHAKPKPSSGANTAQPRTRRRVYTRRHHRDHNDGSDTLCALIYCNQNHTARVLF 509
            |..|:.|     |.|                                   .:|:.|:..|||.|.
plant   481 SVFRLEP-----HMK-----------------------------------CIIFVNRIVTARTLS 505

  Fly   510 ELLAEISRRDPDLKFLRCQYTTDRVADPTTEPKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEE 574
            .:|       .:|:.|| .:.:|.:...::..|  .:..|..|.:||||:..:.|:|:.|.|.||
plant   506 CIL-------NNLELLR-SWKSDFLVGLSSGLK--SMSRRSMETILKRFQSKELNLLVATKVGEE 560

  Fly   575 GIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPSYKSPTVGSVQLTDRSHRYICAT 639
            |:|:..|.||:|:|.|.|..|::|.:||||
plant   561 GLDIQTCCLVIRYDLPETVTSFIQSRGRAR----------------------------------- 590

  Fly   640 GDTTEADSDSDDSAMPNSSGSDPYTFGTARGTVKILNPEVFSKQPPTACDIKLQEIQDELPAAAQ 704
                          ||.|.    |.|....|..|                              :
plant   591 --------------MPQSE----YAFLVDSGNEK------------------------------E 607

  Fly   705 LDTSNSSDEAVSMSNTSPSESSTEQKSRRFQCELSSLTEPEDTSDTTAEIDTAHSLASTTKDLVH 769
            :|...:........|...:..|:|:...|...||..                           ||
plant   608 MDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYK---------------------------VH 645

  Fly   770 QMAQYREIEQMLLSKCANTEPPEQEQSEAERFSACLAAYRPKPHLLTGASVDLGSAIALVNKYCA 834
            :                                             |||.:..||:|:|:.|||:
plant   646 E---------------------------------------------TGACISGGSSISLLYKYCS 665

  Fly   835 RLPSDTFTKLTALWRCTRNERAGVTLFQYTLRLPINSPLKHDIVGLPMPTQTLARRLAALQACVE 899
            |||.|.|.:....::....:..|.|:.:.|  ||.|:|:. :|....:|:...|::.|.|:|..|
plant   666 RLPHDEFFQPKPEFQFKPVDEFGGTICRIT--LPANAPIS-EIESSLLPSTEAAKKDACLKAVHE 727

  Fly   900 LHRIGELDDQLQPIGKEGFRALEPDWECFELEPEDEQIVQLSDEPRPGTTKRRQYYYKRIASEFC 964
            ||.:|.|:|.|.|..|:              |.|||    |||:         ::.:..|..|.|
plant   728 LHNLGVLNDFLLPDSKD--------------EIEDE----LSDD---------EFDFDNIKGEGC 765

  Fly   965 DCRPVAGAPCYLYFIQLTLQCPIPEEQNTRGRKIYPPEDAQQGFGILTTKRIPKLSAFSIFTRSG 1029
                                        :||.              |...|:|
plant   766 ----------------------------SRGD--------------LYEMRVP------------ 776

  Fly  1030 EVKVSLELAKERVILTSEQIVCINGFLNYTFTNVLRLQKFLMLFDPDSTENCVFIVPTVKAPAGG 1094
                                             ||..||:      |.:.:||.:
plant   777 ---------------------------------VLFKQKW------DPSTSCVNL---------- 792

  Fly  1095 KHIDWQFLELIQANGNTMPRAVPDEERQAQPFDPQRFQDAVVMPWYRNQDQPQYFYVAEICPHLS 1159
                                                                 :.|.....||
plant   793 -----------------------------------------------------HSYYIMFVPH-- 802

  Fly  1160 PLSCFPGDNYRTFKHY-YLVKYGLTIQNTSQPLLDVDHTSARLNFLTPRYVNRKGVALPTSSEET 1223
                 |.|  |.:|.: :.:|..|.::..:   :|:|     |:....|.|:.|  ..|:...|
plant   803 -----PAD--RIYKKFGFFMKSPLPVEAET---MDID-----LHLAHQRSVSVK--IFPSGVTE- 849

  Fly  1224 KRAKRENLEQKQILVPELCTVHPFPASLWRTAVCLPCILYRINGLLLADDIRKQVSADLGLGRQQ 1288
                   .:..:|.:.||.           ..:.|..:..|  |.|:.|.:
plant   850 -------FDNDEIRLAELF-----------QEIALKVLFER--GELIPDFV-------------- 880

  Fly  1289 IEDEDFEWPMLDFGWSLSEVLKKSRESKQKESLKDDTINGKDLADVEKKPTSEETQLDKDSKDDK 1353
                    |:        |:...||.||....|
plant   881 --------PL--------ELQDSSRTSKSTFYL-------------------------------- 897

  Fly  1354 VEKSAIELIIEGEEKLQEADDFIEIGTWSNDMADDIASFNQEDDDEDDAFHLPVLPANVKFCDQQ 1418
                .:.|.:...|.:...|       |                            ..::.|
plant   898 ----LLPLCLHDGESVISVD-------W----------------------------VTIRNC--- 920

  Fly  1419 TRYGSPTFWDVSNGESGFKGPKSSQNKQGGKGKAKGPAKPTFNYYDSDNSLGSSYDDDDNAGPLN 1483
              ..||.|...|........|..|..|.         |...:|..|..|||
plant   921 --LSSPIFKTPSVLVEDIFPPSGSHLKL---------ANGCWNIDDVKNSL-------------- 960

  Fly  1484 YMHHNYSSDDDDVADDIDAGRIAFTSKNEAETIETAQEVEKRQKQLSIIQATNANERQYQQTKNL 1548
             :...||.....|| ||..||..|:...|:.|....:.:.|
plant   961 -VFTTYSKQFYFVA-DICHGRNGFSPVKESSTKSHVESIYK------------------------ 999

  Fly  1549 LIGFNFKHEDQKEPATIRYEESIAKLKTEIESGGMLVPHDQQLVLKRSDA-AEAQVAKVSMMELL 1612
            |.|...||     ||     :.:.::|.        :.|.:.|:..|... .|.|......:|:.
plant  1000 LYGVELKH-----PA-----QPLLRVKP--------LCHVRNLLHNRMQTNLEPQELDEYFIEIP 1046

  Fly  1613 KQLLPYVNEDVLAKKLGDRRELLLSDLVELNADWVARHEQETYNVMGCGDSFDNYNDHHRLNLDE 1677
            .: |.::....|:|.:|....||.|.:          |..|...|
plant  1047 PE-LSHLKIKGLSKDIGSSLSLLPSIM----------HRMENLLV-------------------- 1080

  Fly  1678 KQLKLQYERIEIEPPTSTKAITSAILPAGFSFDRQPDLVGHPGPSPSIILQALTMSNANDGINLE 1742
                    .||:      |.:.||.:|      ...::.||.      :|:|||....::.::||
plant  1081 --------AIEL------KHVLSASIP------EIAEVSGHR------VLEALTTEKCHERLSLE 1119

  Fly  1743 RLETIGDSFLKYAITTYLYITYENVHEGKLSHLRSKQVANLNLYRLGRRKRLGEYMIATKFEP-- 1805
            |||.:||:|||:|::.:|::.::::.||:|:..||..|.|.||.||..:|.|..|:.....:|
plant  1120 RLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQ 1184

  Fly  1806 -----HDNWLPPCYYVPKELEKALIEAKIPTHHWKLADLLDIKNLSSVQICEMVREKADALG-LE 1864
                 |     ||.....|                         ::|.::..:.|:    || ||
plant  1185 FFAFGH-----PCRVTCDE-------------------------VASKEVHSLNRD----LGILE 1215

  Fly  1865 QNGGAQNGQLDDSNDSCNDFSCFIPYNLVSQHSIPDKSIADCVEALIGAYLIECGPRGALLFMAW 1929
            .|.|              :..|     ....|.:..|:|||.||||:||:|::.|.:||:.|:.|
plant  1216 SNTG--------------EIRC-----SKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKW 1261

  Fly  1930 LGVRVLPITRQLDGGNQEQR--IPGSTKPNAENVVTVYGAWPTPRSPLLHFAPNATEELDQLLSG 1992
            :||.|...:.|:.......|  :|.:|:.|.|.:
plant  1262 IGVNVDFESLQVQDACIASRRYLPLTTRNNLETL------------------------------- 1295

  Fly  1993 FEEFEESLGYKFRDRSYLLQAMTHASYTPNRL-TDCYQRLEFLGDAVLDYLITRHLYEDPRQHSP 2056
                |..|.|||..:..|:||..|.||  ||. ..||||||||||||||||:|.:.:....:..|
plant  1296 ----ENQLDYKFLHKGLLVQAFIHPSY--NRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKP 1354

  Fly  2057 GALTDLRSALVNNTIFASLAVRHGFHKFFRHLSPGLNDVIDRFVRI---------QQENGHCISE 2112
            |.||||||..|||...|::||.....:|....|..|::||:.:...         |.|...|
plant  1355 GQLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRC--- 1416

  Fly  2113 EYYLLSEEECDDAEDVEVPKALGDVFESIAGAIFLDSNMSLDVVWHVYSNMMSPEIEQFSNSVPK 2177
                              ||.|||:.||..||:|||...:|:.||.:..:.:.| ::..|| :..
plant  1417 ------------------PKVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDP-VKNLSN-LQI 1461

  Fly  2178 SPIRELLEL------EPETAKFGKPEKLADGR---RVRVTVDVFCKGTFRGIGRNYRIAKCTAAK 2233
            |||:||:||      :.|.:...|     ||.   .::||.:..|. |....|||.|.....||:
plant  1462 SPIKELIELCQSYKWDREISATKK-----DGAFTVELKVTKNGCCL-TVSATGRNKREGTKKAAQ 1520

  Fly  2234 CALRQLK 2240
            ..:..||
plant  1521 LMITNLK 1527

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dcr-1	NP_524453.1	DEAD-like_helicase_N	15..208	CDD:421690	45/209 (22%)
		Dicer_PBD	240..344	CDD:277191	25/111 (23%)
		DEAD-like_helicase_C	490..614	CDD:365791	39/123 (32%)
		Dicer_dimer	825..916	CDD:397444	31/90 (34%)
		PAZ_dicer_like	1091..1212	CDD:239209	14/121 (12%)
		RIBOc	1724..1930	CDD:238333	60/213 (28%)
		RIBOc	2008..2172	CDD:238333	63/173 (36%)
		DSRM_DICER	2177..2240	CDD:380680	21/71 (30%)
DCL4	NP_197532.3	MPH1	124..640	CDD:224036	140/746 (19%)
		DEXDc	138..289	CDD:238005	32/164 (20%)
		Helicase_C	470..589	CDD:278689	44/179 (25%)
		Dicer_dimer	657..740	CDD:281376	30/85 (35%)
		PAZ	937..1080	CDD:198017	43/220 (20%)
		RIBOc	1104..1261	CDD:238333	60/209 (29%)
		Rnc	1285..1531	CDD:223644	97/309 (31%)
		RIBOc	1308..1452	CDD:238333	62/166 (37%)
		DSRM	1463..1527	CDD:214634	20/69 (29%)
		DSRM	1621..>1681	CDD:238007
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	62	1.000	Domainoid score	I3750
eggNOG	1	0.900	-	-		E1_COG0571
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57860
OrthoDB	1	1.010	-	-		D1337630at2759
OrthoFinder	1	1.000	-	-		FOG0002051
OrthoInspector	1	1.000	-	-		mtm1149
orthoMCL	1	0.900	-	-		OOG6_101152
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1728
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	11	10.690