Protein Alignment Dcr-1 and DCL3

DIOPT Version :10

• Sequence 1: NP_524453.1
• Gene: Dcr-1 / 42693
• FlyBaseID: FBgn0039016
• Length: 2249
• Species: Drosophila melanogaster
• Sequence 2: NP_001154662.2
• Gene: DCL3 / 823508
• AraportID: AT3G43920
• Length: 1580
• Species: Arabidopsis thaliana
• Alignment Length: 2329
• Identity: 446/2329 - (19%)
• Similarity: 727/2329 - (31%)
• Gaps: 1007/2329 - (43%)

  Fly     7 DNNLHTTVFTPR------DFQVELLATAYERNTIICLGHRSSKEFIALKLLQELSRRARRHGRVS 65
            |.|| .:|.:|.      .:::::...|..||.|..||....|..|..:|::.:. .:....|:.
plant    29 DQNL-DSVSSPMMTDSNGSYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMG-SSDTDKRLI 91

  Fly    66 VYLSCEVGTSTEPCSIYTMLTHLTDLRV-----------WQEQPDMQIPFDHCWTDYHVSILRPE 119
            ::|:..|....:.|   ..:..|.:|:|           |..|     .:|..::.:.|.::.|:
plant    92 IFLAPTVNLVKQQC---CEIRALVNLKVEEYFGAKGVDKWTSQ-----RWDEEFSKHDVLVMTPQ 148

  Fly   120 GFLYLLETRELLLSSVELIVLEDCHDSA---VYQRIRPLFENHIMPAPPADRPRILGLAGPLHSA 181
            ..|.:|.:..|.|..|.|:::::||.:.   .|.::...|.:.     ...:|:|.||
plant   149 ILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKEFYHE-----STSKPKIFGL------- 201

  Fly   182 GCELQQLSAMLATLEQSVLCQIETASDIVTVLR--YCSRPHEYIVQCAPFEMDELSLVLADVLNT 244
                                   |||   .|:|  ..|.|..|..|.:..|.
plant   202 -----------------------TAS---AVIRKGIVSSPSNYAAQVSELER------------- 227

  Fly   245 HKSFLLDHR-YDPYEIYGTDQFMDELKDIPDPKVDPLNVINSLLVVLHEMGPWCTQRAAHHFYQC 308
                |:|.: ::|.|..|.::|...:|:.|               :|:...|.|:...
plant   228 ----LMDSKIFNPEEREGVEKFATTVKEGP---------------ILYNPSPSCSLEL------- 266

  Fly   309 NEKLKVKTPHERHYLLYCLVSTALIQLYSLCEHAFHRHLGSGSDSR-QTIERYSSPKVRRLLQTL 372
              |.|::|.|.:       ...:|.:|..|.:.:|     ...|:: :|.::..|...|.:|..|
plant   267 --KEKLETSHLK-------FDASLRRLQELGKDSF-----LNMDNKFETYQKRLSIDYREILHCL 317

  Fly   373 RCFKPEEVHTQADGLRRM-RHQVDQADFNRLSHTLE-----SKCRMVDQMDQPPTETRALVATLE 431
                        |.|..: .|...:....::|.|.|     .:|.||.:            ..||
plant   318 ------------DNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCK------------EFLE 358

  Fly   432 QILHT-----------TEDRQTNRSAARVTPTPTPAHAKPKPSSGANTAQPRTRRRVYTRRHHRD 485
            .||.|           ..|.|.|..:|.::     .|..||            .:.::   |..|
plant   359 DILSTIGVYLPQDDKSLVDLQQNHLSAVIS-----GHVSPK------------LKELF---HLLD 403

  Fly   486 HNDGSDTLCALIYCNQNHTARVLFELLAEISRRDPDLKFLRCQYTTDRVADPTTEPKEAELEHRR 550
            ...|......||...:..||:|:...:    :::..|.:|...|.|:.  :|:|.....::
plant   404 SFRGDKQKQCLILVERIITAKVIERFV----KKEASLAYLNVLYLTEN--NPSTNVSAQKM---- 458

  Fly   551 QEEVLKRFRMHDCNVLIGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVA 615
            |.|:...|:....|:|..|.|:|||..||.|:.:|.:|.|.|..||.|.:..|
plant   459 QIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHA------------ 511

  Fly   616 PSYKSPTVGSVQLTDRSHRYICATGDTTEADSDSDDSAMPNSSGSDPYTFGTARGTVKILNPEVF 680
               |.....|:...:|        |:..:.|...|                       ::..||.
plant   512 ---KQSNSKSIMFLER--------GNPKQRDHLHD-----------------------LMRREVL 542

  Fly   681 SKQPPTACDIKLQEIQDELPAAAQLDTSNSSDEAVSMSNTSPSESSTEQKSRRFQCELSSLTEPE 745
            .:.|                            ||.::.:..|     ..|:.....|:.|:..|:
plant   543 IQDP----------------------------EAPNLKSCPP-----PVKNGHGVKEIGSMVIPD 574

  Fly   746 DTSDTTAEIDTAHSLASTTKDLVHQMAQYREIEQMLLSKCANTEPPEQEQSEAERFSACLAAYRP 810
            .....:.|       |::|:.:                    ::||
plant   575 SNITVSEE-------AASTQTM--------------------SDPP------------------- 593

  Fly   811 KPHLLTGASVDLGSAIALVNKYCARLPSDTFTKLTALWRCTRNERAGVTLFQYTLRLPINSPLKH 875
                                                    :|||:
plant   594 ----------------------------------------SRNEQ-------------------- 598

  Fly   876 DIVGLPMPTQTLARRLAALQACVELHRIGELDDQ-LQPIGKEGFRALEPDWECFELEPEDEQIVQ 939
                              |..|.:|    .||:. ||..|||...:.:                .
plant   599 ------------------LPPCKKL----RLDNNLLQSNGKEKVASSK----------------S 625

  Fly   940 LSDEPRPGTTKRRQYYYKRIASEFCDC--RPVAGAPCYLYFIQLTLQCPIPEEQNTRGRKIYPPE 1002
            .|.....|:.||::.:....|:.....  ..:.||....|  :....|.|..|       :|
plant   626 KSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAY--KFDFCCNISGE-------VY--- 678

  Fly  1003 DAQQGFGILTTKRIPKLSAFSIFTRS------GEVKVSLELAKERVILTSEQIVCINGFLNYTFT 1061
                             |:||:...|      |:|::.|.|.::
plant   679 -----------------SSFSLLLESTLAEDVGKVEMDLYLVRK--------------------- 705

  Fly  1062 NVLRLQKFLMLFDPDSTENCVFIVPTVKAPAGGKHIDWQFLELIQANGNTMPRAVPDEERQAQPF 1126
                                  :|....:|.|...:..:  ||::|
plant   706 ----------------------LVKASVSPCGQIRLSQE--ELVKA------------------- 727

  Fly  1127 DPQRFQDAVVMPWYRNQDQPQYFYVAEICPHLSPLSCFPGDNYRTFKHYYLVKYGLTIQNTSQPL 1191
              :.||              |:|              |.|...:.|       .|...|.|
plant   728 --KYFQ--------------QFF--------------FNGMFGKLF-------VGSKSQGT---- 751

  Fly  1192 LDVDHTSARLNFLTPRYVNRKGVALPTSSEETKRAKRENLEQKQILVPELCTVHPFPASLWRTAV 1256
                                               |||.|.|..            .:|||.
plant   752 -----------------------------------KREFLLQTD------------TSSLWH--- 766

  Fly  1257 CLPCILYRINGLLL---ADDIRKQVSADLGLGRQQIEDEDFEWPMLDFGWSLSEVLKKSRESKQK 1318
              |..::    |||   .:|:....:.|              |..::...|:.|.|||:
plant   767 --PAFMF----LLLPVETNDLASSATID--------------WSAINSCASIVEFLKKN------ 805

  Fly  1319 ESLKDDTINGKDLADVEKKPTSEETQLDKDSKDDKVEKSAIELIIEGEEKLQEADDFIEIGTWSN 1383
                       .|.|:            :||..::...|:      |:|.|  .||.:|
plant   806 -----------SLLDL------------RDSDGNQCNTSS------GQEVL--LDDKME------ 833

  Fly  1384 DMADDIASFNQEDDDEDDAFHLPVLPANVKFCDQQTRYGSPTFWDVSNGESGFKGPKSSQNKQGG 1448
              ..::..|.....|::....|.|:
plant   834 --ETNLIHFANASSDKNSLEELVVI---------------------------------------- 856

  Fly  1449 KGKAKGPAKPTFNYYD-----SDNSLGSSYDDDDNAGPLNYMHHNYSSDDDDVADDIDAGRIAFT 1508
                   |..|...|.     ||:|..|.::.|.::|...|
plant   857 -------AIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATY------------------------ 890

  Fly  1509 SKNEAETIETAQEVEKRQKQLSIIQATNANE-----RQYQQTKNLLIGFNFKHEDQKEPATIRYE 1568
                         .|...|:..|:.| :.|:     :|.....|||:.||              |
plant   891 -------------AEYFNKKYGIVLA-HPNQPLMKLKQSHHAHNLLVDFN--------------E 927

  Fly  1569 ESIAKLKTEIESGGM------LVPHDQQLVLKRSDAAEAQVAKV----SMMELLKQLLPYVNEDV 1623
            |.:  :|||.::|.:      :..|....:|.|.|...|.:..:    |:|..|:.|:
plant   928 EMV--VKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLM------- 983

  Fly  1624 LAKKLGDRRELLLSDLVELNADWVARHEQETYNVMGCGDSFDNYNDHHRLNLDEKQLKLQYERIE 1688
            ||.:|  |.|:                        .|  |.||::
plant   984 LASQL--REEI------------------------DC--SIDNFS-------------------- 1000

  Fly  1689 IEPPTSTKAITSAILPAGFSFDRQPDLVGHPGPSPSIILQALTMSNANDGINLERLETIGDSFLK 1753
            |...:..:|:|:...|..||                                :||||.:|||.||
plant  1001 ISSTSILEAVTTLTCPESFS--------------------------------MERLELLGDSVLK 1033

  Fly  1754 YAITTYLYITYENVHEGKLSHLRSKQVANLNLYRLGRRKRLGEYMIATKFEPHDNWLPPCYY--V 1816
            |..:.:|::.|.:..||:||..|...::|.||:||...::|..|:....|||. .|..|..:  .
plant  1034 YVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPR-RWTAPGQFSLF 1097

  Fly  1817 PKELEKALIEAKIPTHHWKLADLLDIKNLSSVQICEMVREKADALGLEQNGGAQNGQLDDSNDSC 1881
            |...:..:...::|         ||.|..:                  :|...:.|:      ||
plant  1098 PVPCKCGIDTREVP---------LDPKFFT------------------ENMTIKIGK------SC 1129

  Fly  1882 NDFSCFIPYNLVSQHSIPDKSIADCVEALIGAYLIECGPRGALLFMAWLGVRVLPITRQLDGGNQ 1946
            :          :....:..||::||.|||||||.:..|...:|..|.|||:.|
plant  1130 D----------MGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDV------------ 1172

  Fly  1947 EQRIPGSTKPNAENVVTVYGAWPTPRSPLLHFAPNATEELDQLLSGFEEFEESLGYKFRDRSYLL 2011
                  ...||.  ||....     |..|..:.|..    |:|:    |.|..:.::|..:..|.
plant  1173 ------DFDPNL--VVEAIN-----RVSLRCYIPKE----DELI----ELERKIQHEFSAKFLLK 1216

  Fly  2012 QAMTHASYTPNRLTDCYQRLEFLGDAVLDYLITRHLYEDPRQHSPGALTDLRSALVNNTIFASLA 2076
            :|:||:|.   |.:..|:|||||||:|||:||||||:....|..||.:||||||.|||..||.:|
plant  1217 EAITHSSL---RESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVA 1278

  Fly  2077 VRHGFHKFFRH----LSPGLNDVIDRFVRIQQENGHCISEEYYLLSEEECDDA----EDVEVPKA 2133
            |::..|...:.    |...:||                    ||:|.::.|:.    ..::.|||
plant  1279 VKNNLHTHLQRCATVLETQIND--------------------YLMSFQKPDETGRSIPSIQGPKA 1323

  Fly  2134 LGDVFESIAGAIFLDSNMSLDVVWHVYSNMMSPEIEQFSNSVPKSPIRELLELEPETAKFGKPEK 2198
            ||||.||||||:.:|:.:.||.||.|:..::||.:......:|  |.|||.||......|.:.:.
plant  1324 LGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLP--PYRELNELCDSLGYFFRVKC 1386

  Fly  2199 LADGRRVRVTV-----DVFCKGTFRGIGRNYRIAKCTAAKCALRQLKKQGLIAK 2247
            ..||.:.:.|:     ||...|.  |..:..::|...||...|.||:|:.:..|
plant  1387 SNDGVKAQATIQLQLDDVLLTGD--GSEQTNKLALGKAASHLLTQLEKRNISRK 1438

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Dcr-1	NP_524453.1	DEAD-like_helicase_N	15..208	CDD:475120	38/212 (18%)
		Dicer_PBD	240..344	CDD:277191	18/104 (17%)
		P-loop containing Nucleoside Triphosphate Hydrolases	490..614	CDD:476819	32/123 (26%)
		Dicer_dimer	825..916	CDD:460900	11/91 (12%)
		PAZ_dicer_like	1091..1212	CDD:239209	15/120 (13%)
		RIBOc	1724..1930	CDD:238333	52/207 (25%)
		RIBOc	2008..2172	CDD:238333	68/171 (40%)
		DSRM_DICER	2177..2240	CDD:380680	19/67 (28%)
DCL3	NP_001154662.2	DEXHc_dicer	46..237	CDD:350792	47/254 (19%)
		SF2_C_dicer	391..522	CDD:350189	39/170 (23%)
		PAZ_CAF_like	824..962	CDD:239210	40/248 (16%)
		RIBOc	1006..1175	CDD:238333	61/262 (23%)
		RIBOc	1214..1361	CDD:238333	68/169 (40%)
		DSRM_SF	1492..1572	CDD:444671