DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dcr-1 and DCL2

DIOPT Version :9

Sequence 1:	NP_524453.1	Gene:	Dcr-1 / 42693	FlyBaseID:	FBgn0039016	Length:	2249	Species:	Drosophila melanogaster
Sequence 2:	NP_001189798.1	Gene:	DCL2 / 821300	AraportID:	AT3G03300	Length:	1388	Species:	Arabidopsis thaliana

Alignment Length:	2213	Identity:	427/2213 - (19%)
Similarity:	694/2213 - (31%)	Gaps:	951/2213 - (42%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 RDFQVELLATAYERNTIICLGHRSSKEFIALKLLQELSRRARRHGR-VSVYLSCEVGTSTEPCSI 81
            |.:|||.|..|.::|||:.|...|.|..||:.||:..:...|:... ..|:|..:|...|:....
plant    25 RSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQVVLVTQQAEA 89

  Fly    82 YTMLTHLTDLRVWQEQPDMQIPF--DHCWTD----YHVSILRPEGFLYLLETRELLLSSVELIVL 140
            ..|  | |||:|.....||.:.|  ...|..    |.|.::.|...|..|....|.||.::::::
plant    90 LKM--H-TDLKVGMYWGDMGVDFWDSSTWKQEVDKYEVLVMTPAILLDALRHSFLSLSMIKVLIV 151

  Fly   141 EDCHDSA---VYQRIRPLFENHIMPAPPADRPRILGLAGPL-HSAGCELQQLSAMLATLEQSVLC 201
            ::||.:.   .|..|...|.:..:.:..::.|||.|:...| .:.|..|......:..||..:..
plant   152 DECHHAGGKHPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTKGENLDSYWKKIHELETLMNS 216

  Fly   202 QIETASDIVTVLRYC--SRP----HEYIVQCAPFEMDELSLVLADVLNTHKSFLLDHRYDPYEIY 260
            ::.|..:...:..:.  |.|    :::|...:|..        |.::...:...:.||..    .
plant   217 KVYTCENESVLAGFVPFSTPSFKYYQHIKIPSPKR--------ASLVEKLERLTIKHRLS----L 269

  Fly   261 GTDQFMDELKDIPDPKVDPLNVINSLLVVLHEMGPWCTQRAAHHFYQCNEKLKVKTPHERHYLLY 325
            ||........|..:.::  |.:.::|...|.::|....|:||...          :..:....|:
plant   270 GTLDLNSSTVDSVEKRL--LRISSTLTYCLDDLGILLAQKAAQSL----------SASQNDSFLW 322

  Fly   326 ---CLVSTALIQLYSLCEHAFHRHLGSGSDSRQTIERYSSPKVRRLLQTLRCFKPEEVHTQAD-G 386
               .:.|.||::.:  |           ||:.|..       :..:.|.|. :....::..|: |
plant   323 GELNMFSVALVKKF--C-----------SDASQEF-------LAEIPQGLN-WSVANINGNAEAG 366

  Fly   387 LRRMRHQVDQADFNRLSHTLESKCRMVDQMDQPPTETRALVATLEQILHTTEDRQTNRSAARVTP 451
            |.                ||::.|.:         ||....::||.|.
plant   367 LL----------------TLKTVCLI---------ETLLGYSSLENIR----------------- 389

  Fly   452 TPTPAHAKPKPSSGANTAQPRTRRRVYTRRHHRDHNDGSDTLCALIYCNQNHTARVLFELLAEIS 516
                                                       .:|:.::..||.||..|||||.
plant   390 -------------------------------------------CIIFVDRVITAIVLESLLAEIL 411

  Fly   517 RRDPDLKFLRCQYTTDRVADPTTEPKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEEGIDVPKC 581
               |:....:.:|.....:....:.:      ::|.|:::.||....|:::.||:||||:||..|
plant   412 ---PNCNNWKTKYVAGNNSGLQNQTR------KKQNEIVEDFRRGLVNIIVATSILEEGLDVQSC 467

  Fly   582 NLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPSYKSPTVGSVQLTDRSHRYICATGDTTEAD 646
            |||:|:||.:...|::|.:||||...:.::::|..       |.:....|..:|:  :|.....:
plant   468 NLVIRFDPASNICSFIQSRGRARMQNSDYLMMVES-------GDLLTQSRLMKYL--SGGKRMRE 523

  Fly   647 SDSDDSAMPNSSGSDPYTFGTARGTVKILNPEVFSKQPPTACDIKLQEIQDELPAAAQLDTSNSS 711
            ...|.|.:|                           .||.
plant   524 ESLDHSLVP---------------------------CPPL------------------------- 536

  Fly   712 DEAVSMSNTSPSESSTEQKSRRFQCELSSLTEPEDTSDTTAEIDTAHSLASTTKDLVHQMAQYRE 776
                                            |:|:.:....:::
plant   537 --------------------------------PDDSDEPLFRVES-------------------- 549

  Fly   777 IEQMLLSKCANTEPPEQEQSEAERFSACLAAYRPKPHLLTGASVDLGSAIALVNKYCARLPSDTF 841
                                                   |||:|.|.|:::|:..||:|||||.:
plant   550 ---------------------------------------TGATVTLSSSVSLIYHYCSRLPSDEY 575

  Fly   842 TKLTALWRCTRNERAGVTLFQYTLRLPINSPLKHDIVGLPMPTQTLARRLAALQACVELHRIGEL 906
            .|....:...:::.:      .||.||.:.|:|.  |......:.| ::...|:||::||::|.|
plant   576 FKPAPRFDVNKDQGS------CTLYLPKSCPVKE--VKAEANNKVL-KQAVCLKACIQLHKVGAL 631

  Fly   907 DDQLQPIGKEGFRALEPDWECFELEPEDEQIVQLSDEPRPGTTKRRQYYYKRIASEFCDCRPVAG 971
            .|.           |.||....|...:..:.:|.:.|
plant   632 SDH-----------LVPDMVVAETVSQKLEKIQYNTE---------------------------- 657

  Fly   972 APCYLYFIQLTLQCPIPEEQNTRGRKIYPPEDAQQGFGILTTKRIPKLSAFSIFTRSGEVKVSLE 1036
            .|||                       :|||               .:|.||
plant   658 QPCY-----------------------FPPE---------------LVSQFS------------- 671

  Fly  1037 LAKERVILTSEQIVCINGFLNYTFTNVLRLQKFLMLFDPDSTENCVFIVPTVKAPAGGKHIDWQF 1101

plant   672 ----------------------------------------------------------------- 671

  Fly  1102 LELIQANGNTMPRAVPDEERQAQPFDPQRFQDAVVMPWYRNQDQPQYFYVAEICPHLSPLSCFPG 1166
                                 |||                  :...:||:..:.|: ||
plant   672 ---------------------AQP------------------ETTYHFYLIRMKPN-SP------ 690

  Fly  1167 DNYRTFKHYYLVKYGLTIQNTSQPLLDVDHTSARLNFLTPRYVNRKGVALPTSSEETKRAKRENL 1231
               |.| |...|..|..:....    |:.:||.||.       :.:|....|.|    .....:|
plant   691 ---RNF-HLNDVLLGTRVVLED----DIGNTSFRLE-------DHRGTIAVTLS----YVGAFHL 736

  Fly  1232 EQKQILVPELCTVHPFPASLWRTAV--CLPCILYRINGLLLADDIRKQVSADLGLGRQQIEDEDF 1294
            .|:::|   .|  ..|..:|:|..:  .:..::..:|||.|.|
plant   737 TQEEVL---FC--RRFQITLFRVLLDHSVENLMEALNGLHLRD---------------------- 774

  Fly  1295 EWPMLDFGWSLSEVLKKSRESKQKESLKDDTINGKDLADVEKKPTSEETQLDKDSKDDKVEKSAI 1359
                   |.:|..:|..|..|.:...:..:.|...:|       ||.|
plant   775 -------GVALDYLLVPSTHSHETSLIDWEVIRSVNL-------TSHE----------------- 808

  Fly  1360 ELIIEGEEKLQEADDFIEIGTWSNDMADDIASFNQEDDDEDDAFHLPVLPANVKFCDQQTRYGSP 1424
              ::|..|.                                              |.
plant   809 --VLEKHEN----------------------------------------------CS-------- 817

  Fly  1425 TFWDVSNGESGFKGPKSS-------QNKQGGKGKAKGPAKPTFNYYDSDNSLGSSYDDDDNAGPL 1482
                 :||.|.....|..       ||                                    .|
plant   818 -----TNGASRILHTKDGLFCTCVVQN------------------------------------AL 841

  Fly  1483 NYMHHN-YSSDDDDVADDIDAGRIAFTSKNEAETIETAQEVEKRQKQLSIIQATNANERQYQQTK 1546
            .|..|| |......|.:::: |....|.:|..:........|:...||:.:.....|.|
plant   842 VYTPHNGYVYCTKGVLNNLN-GNSLLTKRNSGDQTYIEYYEERHGIQLNFVDEPLLNGR------ 899

  Fly  1547 NLLIGFNFKHEDQKEPATIRYEESIAKLKTEIESGGMLVPHDQQLVLKRSDAAEAQVAKVSM-ME 1610
            ::....::.|              :||.|.|.|       ||::.|....:.....::.:|: |.
plant   900 HIFTLHSYLH--------------MAKKKKEKE-------HDREFVELPPELCHVILSPISVDMI 943

  Fly  1611 LLKQLLPYVNEDVLAKKLGDRRELLLSDLVELNADWVARHEQETYNVMGCGDSFDNYNDHHRLNL 1675
            .....:|.|.:.:        ..||::                             ||
plant   944 YSYTFIPSVMQRI--------ESLLIA-----------------------------YN------- 964

  Fly  1676 DEKQLKLQYERIEIEPPTSTKAITSAILPAGFSFDRQPDLVGHPGPSPSI-ILQALTMSNANDGI 1739
                ||....::.|                                 |:| :|:|:|.....|..
plant   965 ----LKKSIPKVNI---------------------------------PTIKVLEAITTKKCEDQF 992

  Fly  1740 NLERLETIGDSFLKYAITTYLYITYENVHEGKLSHLRSKQVANLNLYRLGRRKRLGEYMIATKFE 1804
            :||.|||:||||||||:...|:......|||.||..:...::|:.|.:.|.:::|..::....||
plant   993 HLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIRDECFE 1057

  Fly  1805 PHDNWLPPCYYVPKELEKALIEAKIPTHHWKLADLLDIKNLSSVQICEMVREKADALGLEQNGGA 1869
            | ..|:.|                                                   .|:..|
plant  1058 P-KGWMVP---------------------------------------------------GQSSAA 1070

  Fly  1870 QNGQLDDSNDSCNDFSCFIPYNLVSQHSIPDKSIADCVEALIGAYLIECGPRGALLFMAWLGVRV 1934
            .:...|...:|.|.:       :.|:.::..||:||.||:||||||.|.|...||:||.|:|::|
plant  1071 YSLVNDTLPESRNIY-------VASRRNLKRKSVADVVESLIGAYLSEGGELAALMFMNWVGIKV 1128

  Fly  1935 LPITRQLDGGNQEQRIPGSTKPNAENVVTVYGAWPTPRSPLLHFAPNATEELDQLLSGFEEFEES 1999
            ...|.::...:..|         ||.:|.|                           |:  .|..
plant  1129 DFTTTKIQRDSPIQ---------AEKLVNV---------------------------GY--MESL 1155

  Fly  2000 LGYKFRDRSYLLQAMTHASYTPNRLTDCYQRLEFLGDAVLDYLITRHLYEDPRQHSPGALTDLRS 2064
            |.|.|.|:|.|::|:||.||....:..||||||||||:|||||||:|||:.....|||.|||:||
plant  1156 LNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTDMRS 1220

  Fly  2065 ALVNNTIFASLAVRHGFHKFFRHLSPGLNDVIDRFVRIQQENGHCISEEYYLLSEEECDDAE--- 2126
            |.|||..:|.:||:...||...:.|..|:..|.|.|                 ||.|....:
plant  1221 ASVNNECYALVAVKANLHKHILYASHHLHKHISRTV-----------------SEFEQSSLQSTF 1268

  Fly  2127 ----DVEVPKALGDVFESIAGAIFLDSNMSLDVVW----HVYSNMMSPEIEQFSNSVPKSPIREL 2183
                |:..||.||||.||:|||||:||..:.:||:    .:...|::||      :|...|:|||
plant  1269 GWESDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPE------TVKLHPVREL 1327

  Fly  2184 LEL 2186
            .||
plant  1328 TEL 1330

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dcr-1	NP_524453.1	DEAD-like_helicase_N	15..208	CDD:421690	54/200 (27%)
		Dicer_PBD	240..344	CDD:277191	17/106 (16%)
		DEAD-like_helicase_C	490..614	CDD:365791	38/123 (31%)
		Dicer_dimer	825..916	CDD:397444	25/90 (28%)
		PAZ_dicer_like	1091..1212	CDD:239209	18/120 (15%)
		RIBOc	1724..1930	CDD:238333	57/206 (28%)
		RIBOc	2008..2172	CDD:238333	73/174 (42%)
		DSRM_DICER	2177..2240	CDD:380680	6/10 (60%)
DCL2	NP_001189798.1	DEXDc	38..192	CDD:238005	42/156 (27%)
		Helicase_C	382..489	CDD:278689	38/175 (22%)
		Dicer_dimer	560..637	CDD:281376	26/96 (27%)
		PAZ_CAF_like	807..937	CDD:239210	33/271 (12%)
		RIBOc	980..1123	CDD:238333	56/201 (28%)
		Rnc	1151..1384	CDD:223644	85/205 (41%)
		RIBOc	1164..1318	CDD:238333	73/176 (41%)
		DSRM	1323..1383	CDD:214634	6/8 (75%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	102	1.000	Domainoid score	I2292
eggNOG	1	0.900	-	-		E1_COG0571
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I346
OMA	1	1.010	-	-		QHG57860
OrthoDB	1	1.010	-	-		D1337630at2759
OrthoFinder	1	1.000	-	-		FOG0002051
OrthoInspector	1	1.000	-	-		mtm1149
orthoMCL	1	0.900	-	-		OOG6_101152
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1728
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	12	11.740