DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment loco and PsGEF

DIOPT Version :9

Sequence 1:	NP_732773.1	Gene:	loco / 42672	FlyBaseID:	FBgn0020278	Length:	1541	Species:	Drosophila melanogaster
Sequence 2:	NP_996333.4	Gene:	PsGEF / 31224	FlyBaseID:	FBgn0264598	Length:	2777	Species:	Drosophila melanogaster

Alignment Length:	1670	Identity:	327/1670 - (19%)
Similarity:	533/1670 - (31%)	Gaps:	547/1670 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    37 PAPISTSTTPSGSNSQQHQRRR--KKRANYNYNGIRTVEVRRGYNGFGFTISGQQPCRLSCIISS 99
            |.||:...     :|..|.|::  :.|...:.|| |..|...||.....|.:.::..|...:|..
  Fly  1092 PIPIANVV-----DSCFHMRKKTTEFRLTVDPNG-RLAESPTGYCAPDLTRTPKRGARRKSLILR 1150

  Fly   100 SPA------EQAGLRSGDFLISVNGLNVSKLPH--------ETVVQL--IG---NSFGSIRMQIA 145
            :|:      .|..|:...||::. .|..:.|..        .|:|.|  ||   .|.||:::...
  Fly  1151 APSLELKAVWQNLLQRQIFLVNA-ALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSL 1214

  Fly   146 ENYYSDSSDEE----NAHATLRGQLLAASLRHKPRFLHHKAKLH---------------RLRNSP 191
            ::.:.....::    |:|..:......|:.|...|...|...:|               |:.|
  Fly  1215 DSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRILN-- 1277

  Fly   192 QKKLNPPEAVEPHKSKSSPDHPTLKPVLEDPPLTANLSKAADVANVSAMVRAVGSAALECRVIVG 256
              |...|::...|.:..               :...|.|....|.::|:.|:..:.:.|...::
  Fly  1278 --KTGTPKSSALHLANW---------------MKGQLDKQQQQARLAAIARSQENVSAEDEQLI- 1324

  Fly   257 YLGTIEMPKQISHSSKLQTVRSCIRKLRQEKRQPTIVLMCITPDSLSLQSSSGGVLATYSSARLN 321
            :....|..::|::.::.|.          |||          ...|:|...:||:     |||.|
  Fly  1325 FNSDSEQDERITYWTRQQL----------EKR----------TKELNLAKENGGL-----SARPN 1364

  Fly   322 F------------------VSSSSESENRFFGLVTSAV---HNTQIE------------------ 347
            |                  :.|:||:|.     |||.:   |:|..:
  Fly  1365 FGGKRLSGVEELSMSATSDIYSTSEAEG-----VTSVISQSHSTTSDSQITVRSSPIVLDKLAVC 1424

  Fly   348 -------EEYEPSAGSAAAAGHISISHSCHVFVIDTKLIEHAQHLQRAHEFRLQCTRDPISN--- 402
                   ::..|........|.::.|.|..|.:.::..::|:|....      :|.:  :||
  Fly  1425 RHCHKNCQQSGPGGARPGGGGGVNNSSSTPVLLCNSLKVQHSQSSPN------RCCK--LSNGIA 1481

  Fly   403 --LCLEFPNNSE-------YVVNLVRSMYT----MRILPPASRSHQADYEAGAGAAAAGGAVAAA 454
              ..:..|.||.       .|.::..|..|    .:::..:||....|.|:.         ||..
  Fly  1482 GATEMANPKNSNRTGTGTGSVTSMSSSTITGELSSKVVASSSRRETGDTESD---------VAQL 1537

  Fly   455 HSPQPSNHSEISTTTS-----NSDSGIGFNNDCTNIS-----DRILVVDFLAAGAGAAAAAHAGP 509
            .:.:.|.....:|..|     ||....|..|.....|     |..:.|..|..|..:|......|
  Fly  1538 ITDEISQSQSEATDDSVGAMPNSSRSAGEGNPVITGSRLRHLDPPVSVSSLPNGHCSAGVGGGSP 1602

  Fly   510 PTYPAPARPLGIVGI------PDNRLTVRA---MPDHSALLRSPPASSSLRRPNLLASFNLIQSP 565
            ...|.|.|...:..:      |.....|:|   :.:.:.::||.|...               |.
  Fly  1603 KPLPPPRRTRVVAQMCQEPPRPKANQQVKAIVTITETARIMRSSPTKG---------------SG 1652

  Fly   566 ATNLTSTRSCDDVLNLFVDDSPRTLAAVASMDDISLHSAAPSLDEGHTFAHPACVPRKMRRSLA- 629
            :.:.|.|.|        |..||...||        .|......|    |.|....|.||.....
  Fly  1653 SGSATGTGS--------VGGSPAKYAA--------CHCRCTPED----FTHAQLSPDKMEHQTCK 1697

  Fly   630 -LETP----TT------PHKLSAQVFG--QPGSRHSL-GFEAIDSVQSSV-SGACMDQTMDTWAS 679
             ||:|    ||      ..:||..:.|  |.....:| |.|.|...::|. :.|.:..|.|:..:
  Fly  1698 LLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLCGQERIPKEENSTPTIAVVPPTPDSVLT 1762

  Fly   680 LQNLH-------KSHKDRTSMSASSSSHCLLEATNSEPDLGNRALPAN----------------- 720
            ..:.|       .|:...||.::::::      |..:|   .:|:..|
  Fly  1763 KTSTHVWDNSGCSSNGGGTSTTSTATT------TTKQP---RQAIIENIPEDSCDESPLDEEPPY 1818

  Fly   721 ---ASPFRRAWGQSSF-RTPRSDKVAKEQQ---QLGQSSPVRRTASMNASDNDMYIKTLMLDSDL 778
               :|..||....||. :.|..|::.:..:   .|....|.....:...::.|        |.|.
  Fly  1819 RPMSSALRRFGTMSSLEKLPSDDRMDEADELDDDLDSFKPNGHDGNSPQNEED--------DEDA 1875

  Fly   779 KSSRSQHQLSLLQVPKVLTTPAPPSAITASVAAEGAAQDHGCPSSWAGSFERMLQDAAGMQTFSE 843
            .|.|:..|..|          ...|:....|..||.|.  |..::.||::   :.|.  |..|.|
  Fly  1876 DSERALVQNDL----------GASSSSGVIVNGEGLAS--GAWTNRAGAY---VSDK--MSFFEE 1923

  Fly   844 FLKKEFSAENIYFW------------TACERYRLLESEADRVAQAREIFAK------------HL 884
              .:.|..:.:..|            ||.|....::..........|::..            .|
  Fly  1924 --SRAFIDKYLGRWNAGDAQQQHQQGTASETDEQMDECTSGATSGEEVWGTPTSGGDNDDQDLQL 1986

  Fly   885 ANN---------SSDPVNVDSQARSLTEEKLADAAPDIFAPAQKQIFSLMKFDSYQRFIRSDLYK 940
            .|:         ||..:|.|.....:.:|.|  .||.:.|...:.:  |.:|  |:|.:.....:
  Fly  1987 INSENTHSSPTKSSTSLNDDDDTELMMDELL--MAPPMTASTIRGL--LPRF--YRRRLEPLFEE 2045

  Fly   941 SCVEAEQKNQPLPYSGLDLDELLK----TNFHLGAFSKLKKSASNAEDRRRKSLLPWHRKTRSKS 1001
            .....|:|.|.      |.|::.|    ||.|.     |:.||.::.|.......|
  Fly  2046 ETESDEEKTQQ------DSDDIKKGEATTNGHY-----LEDSAGSSSDSESVPPAP--------- 2090

  Fly  1002 RDRTEIMADMQHALMPAPPVPQNAPLTSASLKLVCGQ------NSLSDLHSSRSSLSSFDAGTAT 1060
                        .|:|.|.||......||.     ||      ....|||||...|....|.:|.
  Fly  2091 ------------PLVPRPVVPDREEDPSAE-----GQQENLAMEDQEDLHSSVERLLPNLATSAM 2138

  Fly  1061 GGQGASTESVYSLCRVILTDGATTIVQTRPGETVGELVERLLEKRNLVYPYYDIVFQGS--TKSI 1123
            |.:...|      .|::.|   |.|....|...|....|.||::|....|..|:. .||  |.::
  Fly  2139 GPRPLLT------TRLLPT---TAIDPQPPAVLVASSCEYLLDQRPSSVPGRDLA-GGSIGTTTM 2193

  Fly  1124 DVQQPSQILAGKEVVIERRVAFKLDLPDPKVISVKSKPKKQLHEVIRPILSKYNYKMEQVQVIMR 1188
            ...:|:.:...|...
  Fly  2194 TSSRPTPLSPAKAAA-------------------------------------------------- 2208

  Fly  1189 DTQVPIDLNQPVT------------MADGQRLRIVMVNSDFQVGGG--SSMPPKQSKPMKPLPQG 1239
               .||.:|..:|            .|....:..:...||....||  |..||:...|..| |:.
  Fly  2209 ---TPIGMNATMTTKTTTTTMMTPSRAPSSTISTISSASDTTSSGGRASGRPPRFVPPPPP-PRR 2269

  Fly  1240 HL----DELTNKVFNELLASKADAA-----------ASEKSRPVDLCSMKSNEAPSETSSLFERM 1289
            .|    |.:..:...||...|:.|.           |:..|.|..:.|......|| ||||...:
  Fly  2270 LLLTQTDLIAGQAKTELPRKKSGATADSGCSVAVGDAASASDPTAIGSRTMPSTPS-TSSLASAV 2333

  Fly  1290 R-----------------RQQRDGGNIPASKLPKLKKKSTSSSQQSE-------EAATTQAVADP 1330
            .                 |......:..||::.|..::|..:..||:       .|....:.|.|
  Fly  2334 APKRPSSTIIETCLLGAPRPASATSSATASRMAKPIRQSPPTQHQSQPRGQSCPAAVPAPSTAAP 2398

  Fly  1331 KKPIIAKLKAGVKL--QVTERVAEHQDELLEGLKRAQLARLED----QRG----TEINFDLPDF- 1384
            ..|.:....||..|  ::..|:|.:.|.|.:          ||    :.|    |.:..||..|
  Fly  2399 DAPTVPMPHAGCGLSPRLEMRLALNHDILGD----------EDLICYEPGPDLTTILGHDLSTFH 2453

  Fly  1385 -LKNKENLS-AAVSKLRKVRASLS----PVSKVPATPTEIPQPAP 1423
             |..::.|| :|.::::...|.:|    ..||:. |||...:|.|
  Fly  2454 RLTGRDLLSRSATNRVQPKEAVISYSQQRNSKMD-TPTVNRRPRP 2497

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
loco	NP_732773.1	PDZ_signaling	69..145	CDD:238492	22/94 (23%)
		PTB_RGS12	251..391	CDD:269984	31/185 (17%)
		RGS_R12-like	828..941	CDD:188661	25/145 (17%)
		RGS12_RBD	1073..1145	CDD:176412	17/73 (23%)
		RBD	1144..1213	CDD:128731	5/80 (6%)
PsGEF	NP_996333.4	DUF4799	<102..209	CDD:292674
		C2	286..>381	CDD:278593
		PDZ_signaling	644..716	CDD:238492
		RhoGEF	831..1000	CDD:295373
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3528
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	1	0.900