DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PyK and PKLR

DIOPT Version :9

Sequence 1:	NP_524448.3	Gene:	PyK / 42620	FlyBaseID:	FBgn0267385	Length:	533	Species:	Drosophila melanogaster
Sequence 2:	NP_000289.1	Gene:	PKLR / 5313	HGNCID:	9020	Length:	574	Species:	Homo sapiens

Alignment Length:	512	Identity:	307/512 - (59%)
Similarity:	389/512 - (75%)	Gaps:	3/512 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 AAGADTQLEHMCRLQFDSPVPHVRLSGIVCTIGPASSSVEMLEKMMATGMNIARMNFSHGSHEYH 87
            ||.|||.|||:|.|..||.....|.:.|:.||||||.|||.|::|:..||||||:||||||||||
Human    63 AAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYH 127

  Fly    88 AATVANVRQAVKNYS-AKLGYEHPVAIALDTKGPEIRTGLIGGSGTAEIELKKGEKIKLTTNKEF 151
            |.::||||:||:::: :.|.| .||||||||||||||||::.|...:|:||.||.::.:|.:..|
Human   128 AESIANVREAVESFAGSPLSY-RPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAF 191

  Fly   152 LEKGSLEIVYVDYENIVNVVKPGNRVFVDDGLISLIVREVGKDSLTCEVENGGSLGSRKGVNLPG 216
            ..:|:...|:|||.|||.||..|.|:::|||||||:|:::|.:.|..:|||||.|||||||||||
Human   192 RTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPG 256

  Fly   217 VPVDLPAVSEKDKSDLLFGVEQEVDMIFASFIRNAAALTEIRKVLGEKGKNIKIISKIENQQGMH 281
            ..||||.:||:|..||.||||..||::||||:|.|:.:..:|..||.:|..|||||||||.:|:.
Human   257 AQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVK 321

  Fly   282 NLDEIIEAGDGIMVARGDLGIEIPAEKVFLAQKAMIARCNKAGKPVICATQMLESMVKKPRPTRA 346
            ..|||:|..||||||||||||||||||||||||.||.|||.|||||:|||||||||:.|||||||
Human   322 RFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRA 386

  Fly   347 EISDVANAVLDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHQNLFNDLVRGAG-TIDA 410
            |.|||||||||||||:|||||||||.:|:|.|.......:|||||::|:.||.:|.|.|. :.|.
Human   387 ETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDP 451

  Fly   411 SHAAAIAAVEAATKAKASAIVVITTSGKSAFQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLI 475
            :...||.|||||.|..|:||:|:||:|:||..:|:||||..:|||||.||.|||.||.||:.||:
Human   452 TEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLL 516

  Fly   476 YKEPGLGDWLKDVDVRVQFGLQVGKKNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTV 532
            |:||....|..|||.|||||::.||..||::.||.|:|||||:.|||:||.:|::::
Human   517 YREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSI 573

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PyK	NP_524448.3	Pyruvate_Kinase	46..532	CDD:238178	295/487 (61%)
PyK	NP_524448.3	pyruv_kin	46..529	CDD:273424	294/484 (61%)
PKLR	NP_000289.1	Pyruvate_Kinase	85..573	CDD:238178	295/488 (60%)
		Fructose-1,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGF	475..480		3/4 (75%)
		Fructose-1,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGF	559..564		2/4 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165147008
Domainoid	1	1.000	490	1.000	Domainoid score	I375
eggNOG	1	0.900	-	-		E1_COG0469
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	667	1.000	Inparanoid score	I758
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53565
OrthoDB	1	1.010	-	-		D219692at33208
OrthoFinder	1	1.000	-	-		FOG0000841
OrthoInspector	1	1.000	-	-		otm41027
orthoMCL	1	0.900	-	-		OOG6_100258
Panther	1	1.100	-	-	O	PTHR11817
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2173
SonicParanoid	1	1.000	-	-		X374
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	15	14.800