Protein Alignment PolA1 and REV3

DIOPT Version :10

• Sequence 1: NP_536736.2
• Gene: PolA1 / 42553
• FlyBaseID: FBgn0259113
• Length: 1488
• Species: Drosophila melanogaster
• Sequence 2: NP_015158.1
• Gene: REV3 / 855936
• SGDID: S000006088
• Length: 1504
• Species: Saccharomyces cerevisiae
• Alignment Length: 1509
• Identity: 300/1509 - (19%)
• Similarity: 527/1509 - (34%)
• Gaps: 437/1509 - (28%)

  Fly    63 YGDDWIEEDGTGYAEDLRDFFEDEDEYSDGEE-----DRKDSKKKKGVAPNSKKRPRENEKPVTG 122
            :|..||..|...:...:.:...|.|:.:..::     ||....|...::        ..:.|..|
Yeast   206 FGCSWINVDRCYFRSPVLNSILDIDKLTINDDLQLLLDRFCDFKCNVLS--------RRDFPRVG 262

  Fly   123 KASIKNLFSNAVPKKMDVKTSVKDDDILADI---LGEIKEEPAATSEKAEKVIAPAKISVTSRKF 184
            ...|:   .:.:|:.:..:..::..||..|.   ||:|.:.|
Yeast   263 NGLIE---IDILPQFIKNREKLQHRDIHHDFLEKLGDISDIP----------------------- 301

  Fly   185 DAAAAKEYMNS---FLNNIKVQEQERKKAEASSDNEMLERILKPKAAVPNTKVAFFSSPTIKKEP 246
                .|.|::|   .:|.:.:|.:|....|.....|....:...:........||:
Yeast   302 ----VKPYVSSARDMINELTMQREELSLKEYKEPPETKRHVSGHQWQSSGEFEAFY--------- 353

  Fly   247 MPEKTPAKKATEDPFSDNEMDFSCLDDDENQFDVEKTQQTEKVSQTKTAAEKTSQSKVAEKSAPK 311
              :|...|.:|.|....|..:|.    |:||          |.|...|..|...|   .....|:
Yeast   354 --KKAQHKTSTFDGQIPNFENFI----DKNQ----------KFSAINTPYEALPQ---LWPRLPQ 399

  Fly   312 KETTGSPKESESED--ISRLLNNWESICQMDDDFE-------KS--------VLTTEQDSTISSD 359
            .|...:..:.:..|  ::.....:| ||.:|::.|       ||        .:.:.:||||..|
Yeast   400 IEINNNSMQDKKNDDQVNASFTEYE-ICGVDNENEGVKGSNIKSRSYSWLPESIASPKDSTILLD 463

  Fly   360 QQLRFWYWEAYEDPVKMPGEVFLFGRTADGKSVCLRVQNINRVLYLLPRQFLLDPISKEPT---- 420
            .|.:                   :..|.:....|...||      :..::.|...:|...|
Yeast   464 HQTK-------------------YHNTINFSMDCAMTQN------MASKRKLRSSVSANKTSLLS 503

  Fly   421 --KQKVTVAD--------IYKE--FD-SEVANQLKLEFFRSRKVTKSFAHHAIGIEVPQSCDYLE 472
              ::||..|.        :|.|  |. .::.|:|:.|.|                   ...||.:
Yeast   504 RKRKKVMAAGLRYGKRAFVYGEPPFGYQDILNKLEDEGF-------------------PKIDYKD 549

  Fly   473 VHYDGKKPL---PNLSADKKYN-SIAHI-------FGATTNALERFLLDRKIKGPCWLQVTGFKV 526
            ..:.....|   |...|.|::. |..|:       ||..|.::        ...|.:...:.:|.
Yeast   550 PFFSNPVDLENKPYAYAGKRFEISSTHVSTRIPVQFGGETVSV--------YNKPTFDMFSSWKY 606

  Fly   527 SPTP------MSWCN-----------TEVTLTEP---------KNVELVQDKGKPAPPPPLTLLS 565
            :..|      ..|.|           :::::..|         .:|...|.:.|.:....||.|:
Yeast   607 ALKPPTYDAVQKWYNKVPSMGNKKTESQISMHTPHSKFLYKFASDVSGKQKRKKSSVHDSLTHLT 671

  Fly   566 L----NVRTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMCALTRPAVVSWPLDLNFEM 626
            |    |.|:...|..:.:|:.||.......                         ::||||  ::
Yeast   672 LEIHANTRSDKIPDPAIDEVSMIIWCLEEE-------------------------TFPLDL--DI 709

  Fly   627 AKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQL-NVITDQIVAL----------- 679
            |......|||...:    |.|..:.|....::.|.|...:.:: ..:||.::.|
Yeast   710 AYEGIMIVHKASED----STFPTKIQHCINEIPVMFYESEFEMFEALTDLVLLLDPDILSGFEIH 770

  Fly   680 ------------KIPQWSRMGRL---------RLSQSFGKRLLEH-----FVGRMVCDVKRSAEE 718
                        ||.|:..:..|         :||.::|   ..|     ..||.:.::.|:...
Yeast   771 NFSWGYIIERCQKIHQFDIVRELARVKCQIKTKLSDTWG---YAHSSGIMITGRHMINIWRALRS 832

  Fly   719 CIRARSYDLQTLCKQVLKLKESERM-EVNADDLLEMYEKGESITKLISLTMQDNSYLLRLMCELN 782
            .:....|.:::....:|    .:|: ..:.:.|..|:...:|.|:|.::.   |.:|.|  .::|
Yeast   833 DVNLTQYTIESAAFNIL----HKRLPHFSFESLTNMWNAKKSTTELKTVL---NYWLSR--AQIN 888

  Fly   783 IMPLALQITNICGNTMTRTLQG---------GRSERNEFLLLHAFHEKNYIV--PDKKPVSKRSG 836
            |..|..| ..|..|.....|.|         |...:.|..|:.....:::|:  |.||.|
Yeast   889 IQLLRKQ-DYIARNIEQARLIGIDFHSVYYRGSQFKVESFLIRICKSESFILLSPGKKDV----- 947

  Fly   837 AGDTDATLSGADATMQTKKKAAYAGGLVLEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTVQQ 901
                            .|:||.....||:||....|:..::::||.||||||:..||.|::|:..
Yeast   948 ----------------RKQKALECVPLVMEPESAFYKSPLIVLDFQSLYPSIMIGYNYCYSTMIG 996

  Fly   902 PVDADELP---------TLPDS-----KTEPGILP----------------LQLKRLVESRKEVK 936
            .|....|.         :||.:     |.:..|.|                ..|..:::.|..:|
Yeast   997 RVREINLTENNLGVSKFSLPRNILALLKNDVTIAPNGVVYAKTSVRKSTLSKMLTDILDVRVMIK 1061

  Fly   937 KLM-AAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHS-RFFAQHLAALVTHKGREILTNTQQ 999
            |.| ...|.:..|:...:.:|:||||.||..||....:.| |.....||..:...|||.|.....
Yeast  1062 KTMNEIGDDNTTLKRLLNNKQLALKLLANVTYGYTSASFSGRMPCSDLADSIVQTGRETLEKAID 1126

  Fly  1000 LVQK---MNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVNKLY-KQLELDIDGVFGCLLLL 1060
            :::|   .|..|||||||||.:........:.:.|||.:.:.|.:.. |.:.|..:.|:...:|:
Yeast  1127 IIEKDETWNAKVVYGDTDSLFVYLPGKTAIEAFSIGHAMAERVTQNNPKPIFLKFEKVYHPSILI 1191

  Fly  1061 KKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLDAV--H 1123
            .||:|  :..|.:|........:.||::.||||    .:...:.::::.:......:.|..:  :
Yeast  1192 SKKRY--VGFSYESPSQTLPIFDAKGIETVRRD----GIPAQQKIIEKCIRLLFQTKDLSKIKKY 1250

  Fly  1124 AQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMNRERNRRYKKGDMVDYV 1188
            .|.|..|.||  |.|....|...|::.....:...:|....|.|..|:|.:.....:..:.:.|:
Yeast  1251 LQNEFFKIQI--GKVSAQDFCFAKEVKLGAYKSEKTAPAGAVVVKRRINEDHRAEPQYKERIPYL 1313

  Fly  1189 ICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLEGTDASRIAECLGM 1253
            :..........:|....:|.  .|.:.|:||:.||:...:.|.:.|:..:            :|:
Yeast  1314 VVKGKQGQLLRERCVSPEEF--LEGENLELDSEYYINKILIPPLDRLFNL------------IGI 1364

  Fly  1254 DPTKFRQNAQRTQRENTEQSE----------------GESLLK-TTLQLYRLC------------ 1289
            :...:.|...:::|.:|..::                ||.|.| .:|||...|
Yeast  1365 NVGNWAQEIVKSKRASTTTTKVENITRVGTSATCCNCGEELTKICSLQLCDDCLEKRSTTTLSFL 1429

  Fly  1290 ----------EPFRFQCVTCKTEQLMASAYR----PGPSNSHIAVLQQCAKSECQTAPIQYLASV 1340
                      :..:..|.||        :||    .|..|.|||  .:|...:|   |:.| :.|
Yeast  1430 IKKLKRQKEYQTLKTVCRTC--------SYRYTSDAGIENDHIA--SKCNSYDC---PVFY-SRV 1480

  Fly  1341 RNQLQLSMRQYVQR 1354
            :.:..|...|.|||
Yeast  1481 KAERYLRDNQSVQR 1494

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PolA1	NP_536736.2	pol2	52..1237	CDD:273159	268/1347 (20%)
		zf-DNA_Pol	1286..1459	CDD:462651	21/95 (22%)
REV3	NP_015158.1	PTZ00166	12..>221	CDD:240301	4/14 (29%)
		DNA_polB_zeta_exo	664..892	CDD:99821	50/270 (19%)
		POLBc_zeta	919..1366	CDD:99917	116/489 (24%)
		zf-C4pol	1398..1479	CDD:464119	22/94 (23%)